CAZyme Information: I317_05009-t43_1-p1

Basic Information

• Species: Kwoniella heveanensis
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella heveanensis
• CAZyme ID: I317_05009-t43_1-p1
• CAZy Family: GH51
• CAZyme Description: dolichyl-phosphate-mannose-protein mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
769		88098.43	9.1926

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KheveanensisCBS569	8002	1296119	131	7871

• Gene Location: location=KV700212:73638-76739(+)

Full Sequence      

MSLAPRKRRTDRQEAPALPQNVRSYNELDEEKRRSPRPSPPDSSIHSELLLSWSLGTLLT
IIAFIVRFYRIGHPSQVVFDEVHFGSFASHYIRREYYFDVHPPLAKMLNGLAAWVVGFNG
DFGFEQIGDDYISHNVPYIGMRSFCAIMGSITVPIVYGIMRESGYPVGIALLSATLVLFD
NGHITQTRLILLDAALVLFMALSLFSYVKFHQYRYQEFTKWWWFWLLSTGAWLACTLGCK
MVGLFTFLTVGAAVLWDLWGILDVKRGQPISYFYRHFAYRVVGLIIVPFFIYLSFFWVHF
KVLKYSGPGDTFMSPAFQETLAGNELLLNSQEIRYFDTITMKHKDTKQFLHSHPDKYPLR
YDDGRISSQGQQVTCYPHNDTNNHWQVLPTKEIPESGRGRVVRHNDVIQLKHVNTNTILL
THDVASPLMPTNQEFTTVDPDDEDRKNDTMFKVSITDAHEGEPWKSLSGHFKLIHMPTKV
VLWTHPKPLPDWAYGQQEVNGNKNQQDRTTSWFVEDIIADGTGNDFKNRTAHVEPKAVKK
RAFIKKFFELQILMLQHNAGLTSSHPYASTPIEWPFCLSGISFWTDNNTNQQIYMIGNLL
GWWVCAISLSVFVGIVIADMLARRRGLDPIEDGIRNRLYRNTGFFLGAWAFHYFPFYLMQ
RQRFLHHYLPAHLASALVAGSVLNFVLVEAVNYPVSFAGAKTRLRPAVRARLGTVGWSVV
LGLLVVVVAVFLFLSPLTYGVTLSGEQVNRRKLLSSWTLHFEAKKHHAD

Enzyme Prediction     

EC	2.4.1.109:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	59	300	5.3e-70	0.9910313901345291

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224839	PMT1	0.0	57	763	28	699	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
396786	PMT	2.12e-78	58	303	2	244	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
406576	PMT_4TMC	2.04e-64	545	755	1	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
397103	MIR	4.62e-18	342	514	4	185	MIR domain. The MIR (protein mannosyltransferase, IP3R and RyR) domain is a domain that may have a ligand transferase function.
224720	ArnT	1.51e-08	48	270	2	208	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACZ81482.1|GT39	0.0	1	769	1	769
AUB24907.1|GT39	0.0	1	768	1	764
AFR95151.1|GT39	0.0	1	768	1	764
ABG57096.1|GT39|2.4.1.109	0.0	1	768	1	764
UOH81035.1|GT39	0.0	1	768	1	764

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	5.75e-108	38	757	34	727	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P25_B	9.16e-95	17	759	32	753	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_B Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q59X23|PMT4_CANAL	1.33e-217	57	754	52	747	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT4 PE=2 SV=2
sp|P46971|PMT4_YEAST	2.28e-209	57	752	57	752	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PMT4 PE=1 SV=1
sp|O42933|PMT4_SCHPO	4.99e-204	32	762	35	775	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ogm4 PE=3 SV=1
sp|F1Q8R9|POMT2_DANRE	1.70e-115	11	687	28	692	Protein O-mannosyl-transferase 2 OS=Danio rerio OX=7955 GN=pomt2 PE=1 SV=1
sp|Q9UKY4|POMT2_HUMAN	1.76e-112	3	687	14	685	Protein O-mannosyl-transferase 2 OS=Homo sapiens OX=9606 GN=POMT2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999937	0.000063

TMHMM  Annotations     

Start	End
49	71
138	160
167	184
189	208
221	238
242	264
277	299
599	621
642	659
669	691
712	734