CAZyme Information: I308_05087-t35_1-p1

Basic Information

• Species: Cryptococcus gattii VGIV
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus gattii VGIV
• CAZyme ID: I308_05087-t35_1-p1
• CAZy Family: GT2
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1543	KN848869|CGC1	171831.80	4.8520

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CgattiiIND107	6843	1296105	147	6696

• Gene Location: location=KN848869:145420-151299(+)

Full Sequence      

MRANTVALALAALAIAASASPPVRVNLETGWAAPPLVLEILETVYDEYPSSYFPLLHLLS
SLPINSTDESILSSTLDLIQAYSLLPSPSSLSTFHLALSLRSTAPRIEAEYNWYNGAANS
DQKPHSLSFDHTSPSVSSTSSSPRAVFYYVPFAFTSNELLGYLDHHASQYPEFTYTIRYQ
PPLSHEDRKPLSLSGWGAEMALKRMDYLVVDDRATGNTSFQYEGDERTRDESSIFAHVFG
DDPWGDQATPLTPTEIRDIGLKAATLIMSSDDPISALTHLSQDFPKYSAALARQIVVPED
IQSKGRTIAVRGKAKEAIYINGKPFDRDLNAYALLEALRDERQLTVSLTSLGLTPKQSID
LLADPVVGQGQVEDDMGEGLVDASDRIEGGDVIVYWNDIEKDKRYQNWPIHPQGYMRPVY
PGQFHTVRRNTFNLIFALDLSRISSLELIVHSISNMIQRGLPIRFGIVPIFEPGQQDDIS
VQMAKVFWYSVKTFGRRSTRDFLAAIIDATPRQPSNPAPLITDELLRKGYEALSATSEKA
SLTFDDVLASEDWDHHIEKTGNYLKRLLITKKDAENGGMFMNGRFTPNAPTWPNIVTQEM
QSQLSFIQEQASLVMLDAIPEDISTMFYDLPATSKRRSSLVIPVGDNKLKAFNLVDLFEN
DGIEGKLSGEFVYPDGERGTPVTMWIIGDLDSSEGLETVKNGLQHLQTPQCASRLGFIHV
PPAPNQSSCPAGQYCFSTVLYQILSQSALPLAKPSDLLELISDIQHSIKTNLEKGCEIKV
GNQKVDGCGTTFTLSPEDQQRYFEAKPLHGMTFGGWAAGDIAAASEFWKAGIQIAGKLGI
RGGVHLLANGRLVGPITPMTFPLDDFDALEAYEHRKRVKPIIDVLKMMYDDITVFDRPTL
ANLISKVSSIVTAAYKPLDAEGIFAPTQSTRTRYYEKLDNGAMSFKLGDEDMSLLKVAVV
VDPLSEQAQKWSPIIRTLSEMDHVFVSVYLEPEALMEEVKLKRFYRTSIPSRLTFDVDGA
AIAPGLTFNNLPSNPIYTLGLDTPPSWIVSPRTSPYDLDNLLLSSISSPVSVTFQLKQFL
IEGHARESGNIPPRGLQLQLKTLSGDIAADTQVMANLGYLQFRATPGYYTLSIRPGRGEE
VFNLESVGAEGWDSHSVKEVGDGVSLGSFDGKTIYPKFARKEGMEKADVLQESVATPEGL
AKQVYSRMKSIIGLSTNVTPTKSEHADINIFTVASGLLYERFASIMILSVMKHTDSSVKF
WFIGNFLSPTFIAFIPKLAEEYGFQYEFVTYKWPHWLRAQSEKQRIIWAYKILFLDVLFP
MSLDKVIFVDADQIVRTDMKELVDVDLHGRVYGYAPMGNSRKEMEGFRFWKSGYWKEVLR
GRPYHISALYVVDLKKFRQLATGDRLRGQYHALSADPNSLANLDQDLPNSMQDQIPIWTL
DQDWLWCQTWCSDESLATAKTIDLCQNPLTKEPKLVRARQIPEWDVYDQEIAAFAASVSE
EGEESGALAVSVDDLASEGHVSGVEKKEEETERGNDGHIGDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1228	1475	1.3e-127	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1228	1494	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	4.25e-180	1228	1475	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	3.57e-76	381	641	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
132996	Glyco_transf_8	3.79e-55	1228	1475	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.
399437	UDP-g_GGTase	3.83e-52	1082	1191	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV20593.1|GT24	0.0	1	1543	1	1543
KGB74213.2|GT24	0.0	1	1543	1	1582
AAW41515.2|GT24	0.0	1	1543	1	1582
UOH81582.1|GT24	0.0	1	1543	1	1585
AUB22656.1|GT24	0.0	1	1543	1	1582

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	5.61e-287	18	1501	6	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	6.12e-286	18	1501	6	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TRT_A	6.12e-286	18	1501	6	1454	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS8_A	2.64e-253	20	1437	1	1382	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS8_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	1.79e-224	18	1501	6	1220	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	1.16e-217	9	1504	8	1434	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	2.30e-197	18	1500	25	1513	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	2.99e-187	20	1508	44	1536	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	1.43e-186	6	1491	29	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	3.84e-186	6	1491	29	1520	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000239	0.999739	CS pos: 19-20. Pr: 0.9592

TMHMM  Annotations     

There is no transmembrane helices in I308_05087-t35_1-p1.