CAZyme Information: I308_03389-t35_1-p1

Basic Information

• Species: Cryptococcus gattii VGIV
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus gattii VGIV
• CAZyme ID: I308_03389-t35_1-p1
• CAZy Family: GH47
• CAZyme Description: glyoxal oxidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
631	KN848862|CGC1	68795.01	4.3076

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CgattiiIND107	6843	1296105	147	6696

• Gene Location: location=KN848862:648381-650731(+)

Full Sequence      

MLSPSTLLHALSFAALAALPAQAQLANTFQYVGLSGVSAQQMFLGTLNKVYIVDKTEKNN
ATVNGHPAWASEYDLTTNTFRTMDVLSNSFCAGGTVLGNGTWLNAGGNQAITYGGAAMPT
NQQSGQSPYADWDGGKAVRLLDPCDDESCNWVDDPALYMTSRRWYPTLETLEDGSAIIMG
GCEWGGYVNYADNQNNPTVEYFPPKGQPFTLNFLLNTMPVNLFPLVWLLPSGNLFVQAEY
QAEIFDYKNKIEYPINDIPDCVRVYPASAGTAVFPMTPANNWTATIIFCGGTFLESDQWT
TDWNISQYPANDSCVHISPDVDLTWYQNDPLDTGRSMGNFINLPDGRLFYVNGAHTGTAG
YGNQDWCIGESYADHPLYQSWYFDPTQPSGQRWSKAGVSSIPRMYHSSASLLPDGNVIIS
GSNPNADYVDAVNNASYTYFTQYQVEIFYPDYADHVKPNPQGMPSNITYGGDYFNVTLSA
SDLFNSPLNINKTRAVIMRTGFSTHTMNMGQRHVELETSFTTLDDGGGILHVAQLPPNPA
ILAPGPALFFIVVDGIPSNASWIMVGDGIIGEQTLHERSVLPRSQISAQIMAQYGLGGYS
IAATSAAAKSWVSWKLVGASLMGFLGGIVLL

Enzyme Prediction     

No EC number prediction in I308_03389-t35_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA5	22	586	3.8e-231	0.9928952042628775

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
399910	Glyoxal_oxid_N	4.33e-44	72	291	50	243	Glyoxal oxidase N-terminus. This family represents the N-terminus (approximately 300 residues) of a number of plant and fungal glyoxal oxidase enzymes. Glyoxal oxidase catalyzes the oxidation of aldehydes to carboxylic acids, coupled with reduction of dioxygen to hydrogen peroxide. It is an essential component of the extracellular lignin degradation pathways of the wood-rot fungus Phanerochaete chrysosporium.
401164	DUF1929	4.83e-26	464	565	3	91	Domain of unknown function (DUF1929). Members of this family adopt a secondary structure consisting of a bundle of seven, mostly antiparallel, beta-strands surrounding a hydrophobic core. The 7 strands are arranged in 2 sheets, in a Greek-key topology. Their precise function, has not, as yet, been defined, though they are mostly found in sugar-utilising enzymes, such as galactose oxidase.
199882	E_set_GO_C	2.55e-22	452	565	1	103	C-terminal Early set domain associated with the catalytic domain of galactose oxidase. E or "early" set domains are associated with the catalytic domain of galactose oxidase at the C-terminal end. Galactose oxidase is an extracellular monomeric enzyme which catalyzes the stereospecific oxidation of a broad range of primary alcohol substrates and possesses a unique mononuclear copper site essential for catalyzing a two-electron transfer reaction during the oxidation of primary alcohols to corresponding aldehydes. The second redox active center necessary for the reaction was found to be situated at a tyrosine residue. The C-terminal domain of galactose oxidase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
KGB75973.2|AA5_1	0.0	1	631	1	631
AFR96049.1|AA5_1	0.0	1	631	1	631
AUB25915.1|AA5_1	0.0	1	631	1	631
UOH81994.1|AA5_1	0.0	1	631	1	631
CED84971.1|AA5_1	2.62e-275	17	584	47	616

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4UNM_A	3.63e-13	159	565	253	614	Structure of Galactose Oxidase homologue from Streptomyces lividans [Streptomyces lividans],4UNM_B Structure of Galactose Oxidase homologue from Streptomyces lividans [Streptomyces lividans]
2EIB_A	3.75e-13	81	565	217	637	Chain A, Galactose oxidase [Fusarium graminearum]
1GOF_A	4.95e-13	81	565	217	637	NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE [Hypomyces rosellus],1GOG_A NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE [Hypomyces rosellus],1GOH_A NOVEL THIOETHER BOND REVEALED BY A 1.7 ANGSTROMS CRYSTAL STRUCTURE OF GALACTOSE OXIDASE [Hypomyces rosellus],2EIE_A Chain A, Galactose oxidase [Fusarium graminearum],2JKX_A Chain A, GALACTOSE OXIDASE [Fusarium graminearum],2VZ1_A Chain A, GALACTOSE OXIDASE [Fusarium graminearum],2VZ3_A Chain A, Galactose Oxidase [Fusarium graminearum]
1K3I_A	5.06e-13	134	565	260	654	Crystal Structure of the Precursor of Galactose Oxidase [Fusarium sp.]
2EID_A	6.54e-13	81	565	217	637	Chain A, Galactose oxidase [Fusarium graminearum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q01772|GLOX_PHACH	1.90e-76	34	568	34	552	Aldehyde oxidase GLOX OS=Phanerodontia chrysosporium OX=2822231 GN=GLX PE=1 SV=1
sp|Q3HRQ2|GLOX_VITPS	2.21e-59	74	565	80	521	Aldehyde oxidase GLOX OS=Vitis pseudoreticulata OX=231512 GN=GLOX PE=2 SV=1
sp|Q9FYG4|GLOX1_ARATH	2.07e-49	35	565	105	614	Aldehyde oxidase GLOX1 OS=Arabidopsis thaliana OX=3702 GN=GLOX1 PE=2 SV=1
sp|A0A2H4HHY6|GLOX_ARTAN	3.47e-42	60	565	116	593	Putative aldehyde oxidase Art an 7 OS=Artemisia annua OX=35608 PE=1 SV=1
sp|D4AUF1|WSCD1_ARTBC	8.01e-36	44	566	437	904	WSC domain-containing protein ARB_07867 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07867 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000216	0.999758	CS pos: 23-24. Pr: 0.9797

TMHMM  Annotations     

There is no transmembrane helices in I308_03389-t35_1-p1.