CAZyme Information: I306_05418-t38_1-p1

Basic Information

• Species: Cryptococcus gattii VGI
• Lineage: Arthropoda; Insecta; ; Eriococcidae; Cryptococcus; Cryptococcus gattii VGI
• CAZyme ID: I306_05418-t38_1-p1
• CAZy Family: GT32
• CAZyme Description: glucan endo-1,3-alpha-glucosidase agn1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
783	KN848749|CGC3	83105.79	6.9704

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CgattiiEJB2	6908	1296103	145	6763

• Gene Location: location=KN848749:453488-456052(-)

Full Sequence      

MFSQLLVLGLAVMASVDAHPRLGRPSKYPRRSVIEKRATPATTVSGWSYIGCYKDNGDDR
TLSGSKEITSTMTPSACVAYCSGLGYSYAGLEYYDECYCGNSIDSTKLDVDTRCQFACKG
DSSQACGGVTRLGVYYKGGSTSTSATYVGCYKDNGDNRTLNGSKKISNTMTPSVCTSYCS
GLGYAYAGLEYYDECYCGNSLDSSKAADNSQCQYACTGDSSQKCGGVTRLGVYSLGSPTT
TTTTSPASSTTSTSAATYVGCYKDNGDNRTLNGSKKISSSMTASVCNSYCSGLGYAYAGT
EYYDECYCGNTLDSSKAADNSQCQYACAGDSSQKCGGVTRIGVYQLGTSVTATLTTSKAS
STTASTSVSANAAVTSTVGVAAVPKASSTKALYAHHMVGNTYSYTQSIWANDISLASAAG
IDGFALNYGRDTWQPARIADAYAAAKAQGSFKLFLSLDVSSLSCSSASDAATHAASIATY
ANHSAQAMYNSKVLVSTFSGESCTFGQGSVSAGWTYFRSLLTAKGISIYFVPSIFSDIST
YSSDSWMDGEFNWNGGWPTGNTALDTSSDTSYMSALGSKGYMAAVSPCFFTYYSPSSYNK
DWIYRSDDWLLARRMEQIISMRNSFDMAEIISWNDYGESHYIGPIRADQPNSQGWTTGMP
HTAWLNLVAYYAPAFKTGSYPSASDQMILWTRPHPKAATPTAPTCARPSNWNNTDDNLYV
WVALKAAATVTITSGSNSGSWYLQAGVNKVGIASAAGTITGKIVRDSTTVKSYDSSGSFS
YTL

Enzyme Prediction     

EC	3.2.1.59:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH71	393	773	5.7e-119	0.992

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397634	Glyco_hydro_71	2.08e-146	394	772	3	372	Glycosyl hydrolase family 71. Family of alpha-1,3-glucanases.
211418	GH71	1.75e-92	386	678	1	283	Glycoside hydrolase family 71. This family of glycoside hydrolases 71 (following the CAZY nomenclature) function as alpha-1,3-glucanases (mutanases, EC 3.2.1.59). They appear to have an endo-hydrolytic mode of enzymatic activity and bacterial members are investigated as candidates for the development of dental caries treatments.The member from fission yeast, endo-alpha-1,3-glucanase Agn1p, plays a vital role in daughter cell separation, while Agn2p has been associated with endolysis of the ascus wall.
396406	WSC	1.25e-25	148	226	1	80	WSC domain. This domain may be involved in carbohydrate binding.
396406	WSC	6.61e-25	50	128	1	80	WSC domain. This domain may be involved in carbohydrate binding.
396406	WSC	1.33e-24	259	337	1	80	WSC domain. This domain may be involved in carbohydrate binding.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV23630.1|GH71	0.0	1	783	1	783
KGB76302.2|GH71	0.0	1	783	1	783
UOH83099.1|GH71	0.0	1	783	1	786
AFR96695.2|GH71	0.0	1	783	1	786
AUB26644.1|GH71	0.0	1	783	1	786

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FWW_B	1.43e-12	40	132	83	175	Wnt modulator Kremen in complex with DKK1 (CRD2) and LRP6 (PE3PE4) [Homo sapiens]
7BZT_E	2.99e-12	40	132	92	184	Chain E, KRM1 [Homo sapiens],7BZU_E Chain E, KRM1 [Homo sapiens]
6SNW_E	3.05e-12	40	132	95	187	Chain E, Kremen protein 1 [Homo sapiens]
5FWS_A	3.58e-12	40	132	123	215	Wnt modulator Kremen crystal form I at 1.90A [Homo sapiens],5FWT_A Wnt modulator Kremen crystal form I at 2.10A [Homo sapiens],5FWU_A Wnt modulator Kremen crystal form II at 2.8A [Homo sapiens],5FWV_A Wnt modulator Kremen crystal form III at 3.2A [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O13716|AGN1_SCHPO	3.76e-72	381	749	13	376	Glucan endo-1,3-alpha-glucosidase agn1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn1 PE=1 SV=2
sp|O94510|AGN2_SCHPO	2.75e-48	380	753	1	381	Ascus wall endo-1,3-alpha-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=agn2 PE=1 SV=2
sp|B6GX22|MU71A_PENRW	1.01e-45	387	772	33	407	Mutanase Pc12g07500 OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=PCH_Pc12g07500 PE=1 SV=1
sp|D4AUF1|WSCD1_ARTBC	3.99e-38	40	345	56	402	WSC domain-containing protein ARB_07867 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07867 PE=1 SV=1
sp|D4AUF4|WSCD2_ARTBC	3.87e-30	43	234	521	716	WSC domain-containing protein ARB_07870 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_07870 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000451	0.999513	CS pos: 18-19. Pr: 0.9718

TMHMM  Annotations     

There is no transmembrane helices in I306_05418-t38_1-p1.