CAZyme Information: I303_08599-t42_1-p1

Basic Information

• Species: Kwoniella dejecticola
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella dejecticola
• CAZyme ID: I303_08599-t42_1-p1
• CAZy Family: GT90
• CAZyme Description: starch phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
894		101028.71	5.4264

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KdejecticolaCBS10117	8724	1296121	122	8602

• Gene Location: location=KI894038:496240-500251(-)

Full Sequence      

MSRRSSVSAGHPHGLKPVDTTQIGIPVVTPRKERPGHVRSLTGSYFPAQKGVVTMEDEWP
IGDEKTWKTALKDLEFDHEDEQEVANTVVRHVTTSLARQAYNMDAIAAYQATALSVRDRL
LERWNETALHHTKKAPKRVYYLSIEWLIGRSLDNAVLNLGMRNTYEGATQKLGFNFEDLL
NEERDAGLGNGGLGRLAACYIDSMATLNIPGWGYGLSRYNYGIFKQLISNSGEQLEAPDP
WLDRENPWEIGRLDVAYPIRFYGRVESVPNSDKAVWTGGMECLAVAYDTPIPGFATKNCA
NIRLWSAKPVQGFDLNSFNAGNYEASVAASSEVENITRVLYPNDNMYAGKKLRIMQQYLW
VSASLQDILRRFTKLDVPWSALPDYVCIQMNDTHPTLAIPELMRILIDEEELSYEDAWKI
TTKVFAYTNHTVLPEALERWQIDLFESLLPRHLQIIYRINYDFMQAVAKRWPGDMDRMRR
MSIIEEGTPKYVRMAYLAIVGSFKVNGVAELHSQLLQATIFKDFVEFKGRDFFTNVTNGI
TPRRWLLQCNPQLAALITHTLGNDHWLTHLTALKQLLPMAEREEFRKAFTSIKLENKQRL
ADLVEAELGVTLDIESIFMTQIKRLHEYKRQTLNLFAVIYRYLRIKKASPEERKKITKHT
SIFAGKAAPGYYVAKLVIRLINNVAKVVNNDPDVEDLLKVVFIPDYSVSIAEVLVPASDV
SVQISTAGTEASGTSNMKLALNGALLLGTVDGANVEIAEDAGEDQSFLFGHLTEQVEGVR
FTNSYQPTPLEQRSSELAEVFKAVESGTFGEGSTYDSLLKTVYEHDYYLVSNDFGSYLAA
EKLMDETYDNNRTEWTKKSIVTAFNMGDFSSDRSVQDYADGIWSVEPCEVPGNL

Enzyme Prediction     

EC	2.4.1.1:158

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	169	886	1.7e-265	0.9970326409495549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
273967	P_ylase	0.0	88	885	1	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
340853	GT35_Glycogen_Phosphorylase	0.0	85	885	1	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
395272	Phosphorylase	0.0	170	885	2	661	Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin.
184948	PRK14986	0.0	111	887	44	812	glycogen phosphorylase; Provisional
223136	GlgP	0.0	76	887	1	750	Glucan phosphorylase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAW44124.1|GT35	0.0	1	891	33	923
AUB25813.1|GT35	0.0	1	891	32	922
AFR95951.2|GT35	0.0	1	891	32	922
ADV22853.1|GT35	0.0	1	891	32	922
KGB76066.3|GT35	0.0	1	891	32	922

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1YGP_A	2.10e-303	89	887	49	877	Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. [Saccharomyces cerevisiae],1YGP_B Phosphorylated Form Of Yeast Glycogen Phosphorylase With Phosphate Bound In The Active Site. [Saccharomyces cerevisiae]
4BQE_A	3.38e-253	79	891	58	874	Arabidopsis thaliana Cytosolic Alpha-1,4-glucan Phosphorylase (PHS2) [Arabidopsis thaliana],4BQE_B Arabidopsis thaliana Cytosolic Alpha-1,4-glucan Phosphorylase (PHS2) [Arabidopsis thaliana],4BQF_A Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose [Arabidopsis thaliana],4BQF_B Arabidopsis thaliana cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with acarbose [Arabidopsis thaliana],4BQI_A ARABIDOPSIS THALIANA cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with maltotriose [Arabidopsis thaliana],4BQI_B ARABIDOPSIS THALIANA cytosolic alpha-1,4-glucan phosphorylase (PHS2) in complex with maltotriose [Arabidopsis thaliana]
2GJ4_A	3.31e-251	79	887	11	818	Structure of rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	3.42e-251	79	887	11	818	Structure of rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
1C8L_A	6.04e-251	79	887	22	829	Synergistic Inhibition Of Glycogen Phosphorylase A By A Potential Antidiabetic Drug And Caffeine [Oryctolagus cuniculus],1LWN_A Crystal structure of rabbit muscle glycogen phosphorylase a in complex with a potential hypoglycaemic drug at 2.0 A resolution [Oryctolagus cuniculus],1LWO_A Crystal structure of rabbit muscle glycogen phosphorylase a in complex with a potential hypoglycaemic drug at 2.0 A resolution [Oryctolagus cuniculus],2GPA_A Allosteric Inhibition Of Glycogen Phosphorylase A By A Potential Antidiabetic Drug [Oryctolagus cuniculus],3AMV_A Allosteric Inhibition Of Glycogen Phosphorylase A By A Potential Antidiabetic Drug [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P06738|PHSG_YEAST	1.47e-303	89	887	72	900	Glycogen phosphorylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GPH1 PE=1 SV=4
sp|Q00766|PHS1_DICDI	2.65e-299	70	894	31	849	Glycogen phosphorylase 1 OS=Dictyostelium discoideum OX=44689 GN=glpV PE=1 SV=3
sp|P34114|PHS2_DICDI	2.70e-282	79	892	109	914	Glycogen phosphorylase 2 OS=Dictyostelium discoideum OX=44689 GN=glpD PE=1 SV=2
sp|P32811|PHSH_SOLTU	2.53e-256	79	891	21	838	Alpha-glucan phosphorylase, H isozyme OS=Solanum tuberosum OX=4113 PE=1 SV=1
sp|Q9PKE6|PHSG_CHLMU	1.76e-255	109	885	38	809	Glycogen phosphorylase OS=Chlamydia muridarum (strain MoPn / Nigg) OX=243161 GN=glgP PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000029	0.000001

TMHMM  Annotations     

There is no transmembrane helices in I303_08599-t42_1-p1.