CAZyme Information: I302_05401-t42_1-p1

Basic Information

• Species: Kwoniella bestiolae
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella bestiolae
• CAZyme ID: I302_05401-t42_1-p1
• CAZy Family: GH43
• CAZyme Description: glucosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
613	KI894021|CGC10	67423.90	4.3910

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KbestiolaeCBS10118	9270	1296100	137	9133

• Gene Location: location=KI894021:1866782-1869485(-)

Full Sequence      

MSRISQSSLNSTTSMLPNSANNRYASSEYGQSTVGGASGSSRFGPTAQIGGTGTAGTGSG
SSSAFDSSSNGNGLHQRFTHDELQDQDDEMDDHLHTFTAAEQKNVDHSANLNLTSWRGWA
NALTLGFIAMACVGIFALYPIFDFYLLKDENAMGANTAGYNLGGINSTGQYPSVPGLPKL
IDDDTPESARTWTSLQGEEWQLVFSDEFNKDGRTFFPGDDPFFTAVDLHYWGTNDFEWLD
PSAATTKDGHLVITMSQEPIHDLNLKSGQLQSWNQLCFNKNAYVSMSASLPGHSHIGGFW
PGFWLMGNLGRPAYGASTDGEYTYDSCDIGTLANQTWPNGTAPVATLTTGENDGVLSYLP
GQRLSSCTCPGEDHPGPDVSVGRAAPEIDIVEAQIKIAVRHGEVSQSNQVAPYDDHYQFN
NRSGFVEIYDESLTEFNSYLGGYYQQAVSGLTLLPDRIYSNQVVGGRSGEFAEFGVEWSA
HPEEREKGYISWVSDGKRSWTMYADATAENPRTGVGRRIVSEEPMAIVINLHASNNFQFV
DWANMIFPNYFRIDWVRVYQRPDGSGTIGCDPPDYPTKDYIERHLDVYTNPNLTTWVEGA
NKPWPKNRLIDQC

Enzyme Prediction     

No EC number prediction in I302_05401-t42_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	206	559	1.2e-126	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	83	613	3	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	7.80e-144	202	560	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	8.62e-33	168	574	16	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.25e-11	204	559	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	0.001	426	559	103	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AUB25004.1|GH16_2	6.17e-259	88	613	102	622
AFR95233.2|GH16_2	6.17e-259	88	613	102	622
UOH81139.1|GH16_2	6.17e-259	88	613	102	622
ADV24708.1|GH16_2	1.49e-257	3	613	24	623
AAW43075.1|GH16_2	4.89e-256	88	613	103	623

PDB Hits     

I302_05401-t42_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87024|SKN1_CANAX	6.99e-132	89	610	246	735	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P33336|SKN1_YEAST	9.94e-127	89	613	257	763	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	1.32e-123	89	613	217	711	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87023|KRE6_CANAX	8.15e-120	171	610	321	738	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|O13941|YEPB_SCHPO	1.62e-116	118	613	155	627	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000051	0.000001

TMHMM  Annotations     

Start	End
119	141