CAZyme Information: I302_04257-t42_1-p1

Basic Information

• Species: Kwoniella bestiolae
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella bestiolae
• CAZyme ID: I302_04257-t42_1-p1
• CAZy Family: PL14
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
550	KI894020|CGC14	59419.28	7.6563

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KbestiolaeCBS10118	9270	1296100	137	9133

• Gene Location: location=KI894020:2310969-2313259(+)

Full Sequence      

MGNTPSHQHGHGHEHGGAGGGKGNDHLQIGYDDLISPSPTPTSTHHTHSHGHRHHPNLRL
PMPARPTHISPSSSNPTSPSGSNHRSGSPRRRKSLELPDLNKLSFTPSALTPAAPVPTTH
THTSHHLAPSTSAVKNTSPTPQAPVTPTPATGTSQKKWQQVLGGRTASPLANANALGAMS
RIDNPPRSAPIAMPNTNLHSRPPQAEDNNPYFPTTAAQDAAAKKSIPIPIPGKEARSTVT
GQTGLSPPPPTKAPTPLPPPATAESQQPIQPTDDGMVNVPIHWTGGGKNVYVTGNFADNW
KGRIKLHRSTHDFNTILRLPPGQYRLKFIVDDSWMCSKLISTATDDDGTLVNWIEVETPK
TEEEMRAEWAMDAKPAVKEENDDESQWTTTIPPSLTLYQYIEELPNHFSPEEYSLFLKSV
PYLPNVPAPPTLPRILDKVIVNNDSKRLWDSHDPSMANQVGYSGAPATLDDNSILAVPNH
VVLNHLTASAIRNGTLGVGTTTRYRKKYITTMFFKPTLSDMPGTVPTTEPPTQSQSEADS
VSVSQPQSVQ

Enzyme Prediction     

No EC number prediction in I302_04257-t42_1-p1.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
406865	AMPK1_CBM	4.85e-36	277	362	1	85	Glycogen recognition site of AMP-activated protein kinase. AMPK1_CBM is a family found in close association with AMPKBI pfam04739. The surface of AMPK1_CBM reveals a carbohydrate-binding pocket.
214973	AMPKBI	5.93e-26	381	516	1	99	5'-AMP-activated protein kinase beta subunit, interation domain. This region is found in the beta subunit of the 5'-AMP-activated protein kinase complex, and its yeast homologues Sip1, Sip2 and Gal83, which are found in the SNF1 kinase complex. This region is sufficient for interaction of this subunit with the kinase complex, but is not solely responsible for the interaction, and the interaction partner is not known. The isoamylase N-terminal domain is sometimes found in proteins belonging to this family.
199889	E_set_AMPKbeta_like_N	7.57e-25	278	356	1	80	N-terminal Early set domain, a glycogen binding domain, associated with the catalytic domain of AMP-activated protein kinase beta subunit. E or "early" set domains are associated with the catalytic domain of AMP-activated protein kinase beta subunit glycogen binding domain at the N-terminal end. AMPK is a metabolic stress sensing protein that senses AMP/ATP and has recently been found to act as a glycogen sensor as well. The protein functions as an alpha-beta-gamma heterotrimer. This N-terminal domain is the glycogen binding domain of the beta subunit. This domain is also a member of the CBM48 (Carbohydrate Binding Module 48) family whose members include pullulanase, maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, and isoamylase.
398422	AMPKBI	1.14e-23	427	516	1	69	5'-AMP-activated protein kinase beta subunit, interaction domain. This region is found in the beta subunit of the 5'-AMP-activated protein kinase complex, and its yeast homologs Sip1, Sip2 and Gal83, which are found in the SNF1 kinase complex. This region is sufficient for interaction of this subunit with the kinase complex, but is not solely responsible for the interaction, and the interaction partner is not known. The isoamylase N-terminal domain (pfam02922) is sometimes found in proteins belonging to this family.
199878	E_set	0.008	283	334	7	60	Early set domain associated with the catalytic domain of sugar utilizing enzymes at either the N or C terminus. The E or "early" set domains of sugar utilizing enzymes are associated with different types of catalytic domains at either the N-terminal or C-terminal end. These domains may be related to the immunoglobulin and/or fibronectin type III superfamilies. Members of this family include alpha amylase, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase. A subset of these members were recently identified as members of the CBM48 (Carbohydrate Binding Module 48) family. Members of the CBM48 family include pullulanase, maltooligosyl trehalose synthase, starch branching enzyme, glycogen branching enzyme, glycogen debranching enzyme, isoamylase, and the beta subunit of AMP-activated protein kinase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV22772.1|CBM48	6.84e-161	57	515	27	490
AFR95838.1|CBM48	1.38e-152	57	515	27	490
AUB25695.1|CBM48	1.38e-152	57	515	27	490
UOH82189.1|CBM48	6.29e-151	57	515	27	490
AAW44123.1|CBM48	8.96e-140	124	515	92	490

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2QLV_B	3.47e-25	276	516	3	251	Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1 [Saccharomyces cerevisiae],2QLV_E Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1 [Saccharomyces cerevisiae]
4QFG_B	3.52e-24	281	516	16	203	Structure of AMPK in complex with STAUROSPORINE inhibitor and in the absence of a synthetic activator [Rattus norvegicus],4QFR_B Structure of AMPK in complex with Cl-A769662 activator and STAUROSPORINE inhibitor [Rattus norvegicus],4QFS_B Structure of AMPK in complex with Br2-A769662core activator and STAUROSPORINE inhibitor [Rattus norvegicus],5KQ5_B AMPK bound to allosteric activator [Rattus norvegicus],5UFU_B Structure of AMPK bound to activator [Rattus norvegicus]
5T5T_B	4.80e-24	281	516	16	203	AMPK bound to allosteric activator [Rattus norvegicus]
6E4T_B	4.80e-24	281	516	16	203	Structure of AMPK bound to activator [Rattus norvegicus],6E4U_B Structure of AMPK bound to activator [Rattus norvegicus],6E4W_B Structure of AMPK bound to activator [Rattus norvegicus]
6C9F_B	1.67e-23	281	516	16	203	AMP-activated protein kinase bound to pharmacological activator R734 [Homo sapiens],6C9G_B AMP-activated protein kinase bound to pharmacological activator R739 [Homo sapiens],6C9H_B non-phosphorylated AMP-activated protein kinase bound to pharmacological activator R734 [Homo sapiens],6C9J_B AMP-activated protein kinase bound to pharmacological activator R734 [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P78789|AAKB_SCHPO	4.04e-31	270	515	92	294	5'-AMP-activated protein kinase subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=amk2 PE=1 SV=2
sp|P80386|AAKB1_RAT	2.28e-23	281	516	82	269	5'-AMP-activated protein kinase subunit beta-1 OS=Rattus norvegicus OX=10116 GN=Prkab1 PE=1 SV=4
sp|Q5BIS9|AAKB1_BOVIN	2.28e-23	281	516	82	269	5'-AMP-activated protein kinase subunit beta-1 OS=Bos taurus OX=9913 GN=PRKAB1 PE=2 SV=3
sp|P34164|SIP2_YEAST	2.54e-23	276	516	163	411	SNF1 protein kinase subunit beta-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SIP2 PE=1 SV=3
sp|Q9R078|AAKB1_MOUSE	4.21e-23	281	516	82	269	5'-AMP-activated protein kinase subunit beta-1 OS=Mus musculus OX=10090 GN=Prkab1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000059	0.000000

TMHMM  Annotations     

There is no transmembrane helices in I302_04257-t42_1-p1.