CAZyme Information: I302_04047-t42_1-p1

Basic Information

• Species: Kwoniella bestiolae
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella bestiolae
• CAZyme ID: I302_04047-t42_1-p1
• CAZy Family: GH18
• CAZyme Description: dolichyl-phosphate-mannose-protein mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
770	KI894020|CGC10	88203.45	8.9860

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KbestiolaeCBS10118	9270	1296100	137	9133

• Gene Location: location=KI894020:1749489-1752518(-)

Full Sequence      

MSLAPRKRRTERQDSPSGLPQTVRTYSGLEEEKRRSPRPPPPDSSLRSEYFFSWSTGLIL
TILAFIIRFWRIGHPSQVVFDEVHFGSFASHYIRREYYFDVHPPLAKMLNGLAAWVVGFN
GDFGFEQIGDDYISHNVPYVGMRSFCAIMGSITVPIVFGIMRESGYPVGIALLSAALVAF
DNGHITQTRLILLDAALVLFMALSLFSYVKFHQYRYQEFTNWWWFWLLSTGGWLACTLGC
KMVGLFTFLSIGAAVIWDLWEILDIKKGHSMAYVWKHFFYRAVGLIMVPFIIYLSFFWIH
FKVLKYSGPGDSFMSPAFQETLAGNELLLNAQEIRYFDTVTIKHKDTKQFLHSHADHYPL
RYEDGRISSQGQQVTCYPHNDTNNNWQILPTKEIPESGRGRVVRHNDVIQLKHVVTDTIL
LTHDVASPLMPTNQEFTTVAEDDEARRNDTMFKVVITDAHEGEPWRSLSGHFKLVHIPTK
VVLWTHPTALPDWAYGQQEVNGNKNQQDRTTSWFVEDIIADGTGNDFKNRTAHVEPKKTK
KVAFIKKFFELQILMLQHNAGLTSSHPYASTPVEWPFCLSGISFWTDSETNQQIYMIGNL
LGWWTCAISLSVFIGIVTADMLARRRGLDPIEDGIRNRLYRNTGFFLGAWAFHYFPFYLM
QRQRFLHHYLPAHLASALVAGSVLNFILVEVVNYPISYPGSKTRLRPAVRARLGKTGWGV
VVGLLAVVVGMYLWLAPLTYALTLTGEQVNRRKLLSSWTLHFEAKRNHAD

Enzyme Prediction     

EC	2.4.1.109:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	60	301	2.4e-70	0.9910313901345291

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224839	PMT1	0.0	58	764	28	699	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
396786	PMT	6.97e-80	59	304	2	244	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
406576	PMT_4TMC	1.26e-64	546	756	1	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
397103	MIR	1.32e-15	343	515	4	185	MIR domain. The MIR (protein mannosyltransferase, IP3R and RyR) domain is a domain that may have a ligand transferase function.
224720	ArnT	5.07e-08	58	269	11	206	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACZ81482.1|GT39	0.0	1	770	1	769
ADV21700.1|GT39	0.0	1	769	1	764
AUB24907.1|GT39	0.0	1	769	1	764
AFR95151.1|GT39	0.0	1	769	1	764
ABG57096.1|GT39|2.4.1.109	0.0	1	769	1	764

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	5.40e-110	60	758	56	727	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P25_B	1.31e-94	58	760	69	753	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_B Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q59X23|PMT4_CANAL	4.85e-218	59	755	53	747	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT4 PE=2 SV=2
sp|P46971|PMT4_YEAST	1.89e-208	48	687	50	684	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PMT4 PE=1 SV=1
sp|O42933|PMT4_SCHPO	2.13e-197	58	763	63	775	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ogm4 PE=3 SV=1
sp|Q8R2R1|POMT1_MOUSE	2.28e-112	60	685	44	682	Protein O-mannosyl-transferase 1 OS=Mus musculus OX=10090 GN=Pomt1 PE=1 SV=1
sp|F1QF89|POMT1_DANRE	2.31e-112	58	760	19	715	Protein O-mannosyl-transferase 1 OS=Danio rerio OX=7955 GN=pomt1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999964	0.000040

TMHMM  Annotations     

Start	End
50	72
139	161
168	185
190	209
222	239
243	265
278	300
600	622
643	660
675	697
718	740