CAZyme Information: I302_03172-t42_1-p1

Basic Information

• Species: Kwoniella bestiolae
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella bestiolae
• CAZyme ID: I302_03172-t42_1-p1
• CAZy Family: GH154
• CAZyme Description: polygalacturonase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
472		52478.50	4.5533

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KbestiolaeCBS10118	9270	1296100	137	9133

• Gene Location: location=KI894019:3525824-3527440(+)

Full Sequence      

MWAKSLLAGSVLSTALAGVIDLSIPSAGELISDVEAWIHPSSALDFQEDYYDPQAQVKRK
LCTLHALGDEKDDSNNLVAAFEKCGKGGIIRLPDANYTIGRPLDLHLENAVLDLHGWLSF
TADVPYWLENHFDFPFQKQSLAFIIRGNDYILDGNDKGGIDGNGQVWYNYAKDFGNKYGR
PMSLTIKDSKNVLVKNFSIIQPQFWASLVWGSENVYFKDFYVNATSFNPESSSDEKNWLQ
NTDGSDTYQSHNVTYENFVYQGGDDCIALKPNSTLITARNVTCFGGTGIAFGSIAQYAGV
KDIIEDVLLEDIGLYPSDQCPGYQGVYFKSWLGYSVGIPPNGGGGGYGYAKNITVRDVYM
EDIWHPLVVQSDLTYLEFNRKQYADSGLFEWSDIHLKNFTGTALANRVAWMSCSKITPCH
DWTFEDINIKPGKKDHPEIHYTCNNFVLGGNDGLSQCHPSNSTFETDNGGTL

Enzyme Prediction     

No EC number prediction in I302_03172-t42_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	140	445	3.3e-63	0.7846153846153846

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395231	Glyco_hydro_28	1.42e-25	158	446	61	315	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
177865	PLN02218	1.93e-22	148	445	156	417	polygalacturonase ADPG
215426	PLN02793	2.64e-18	158	431	151	396	Probable polygalacturonase
215120	PLN02188	4.42e-18	69	430	48	375	polygalacturonase/glycoside hydrolase family protein
215540	PLN03010	1.45e-14	227	464	199	409	polygalacturonase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADV21054.1|GH28	2.23e-271	22	472	23	474
AUB28044.1|GH28	3.81e-271	33	472	40	479
AFR97960.2|GH28	3.81e-271	33	472	40	479
AAW42245.1|GH28	1.05e-270	30	472	36	478
KGB77456.1|GH28	5.23e-270	31	472	35	474

PDB Hits     

I302_03172-t42_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2UP32|RGXA_ASPOR	3.63e-119	53	430	22	413	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rgxA PE=3 SV=1
sp|B8NJB0|RGXA_ASPFN	3.63e-119	53	430	22	413	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rgxA PE=3 SV=2
sp|A2QBB4|RGXA_ASPNC	2.83e-110	53	445	23	428	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rgxA PE=2 SV=2
sp|Q2EQQ2|RGXA_ASPNG	5.65e-110	53	450	23	433	Putative galacturan 1,4-alpha-galacturonidase A OS=Aspergillus niger OX=5061 GN=rgxA PE=2 SV=1
sp|A2RAY7|RGXC_ASPNC	4.25e-68	61	457	29	423	Putative galacturan 1,4-alpha-galacturonidase C OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rgxC PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000866	0.999100	CS pos: 17-18. Pr: 0.9588

TMHMM  Annotations     

There is no transmembrane helices in I302_03172-t42_1-p1.