CAZyme Information: I302_00774-t42_1-p1

Basic Information

• Species: Kwoniella bestiolae
• Lineage: Basidiomycota; Tremellomycetes; ; Cryptococcaceae; Kwoniella; Kwoniella bestiolae
• CAZyme ID: I302_00774-t42_1-p1
• CAZy Family: AA7
• CAZyme Description: glicosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
883		99333.06	6.3019

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_KbestiolaeCBS10118	9270	1296100	137	9133

• Gene Location: location=KI894018:2043025-2046081(-)

Full Sequence      

MPIVERELYGFTLPSPPKAGSSLTSFELRTNDKGRYKNYVYTLSFPLPNVYRVLLTGPDR
PRPPHDNIVLPDKPLEFKLTSLDDDKCTATFDFPSTPGAAELDGSDRKRVLEISWREHIN
LASYEYISKEKQRVLGDLPNRSYALTEKGVMRHWWIEADNLHLGLGEKAAPIDLSGRSFT
MHGSDSANYDAYNTDPLYKHTPYLISTPKAREGKEVQSTYAIYHPTNSGGLWDIQSLHDD
PWGQFKTYTQDYGGLEEYVLVGKGVQQVVRTFAELVGKPRLVGRDWLGYLASGMGLGESD
HPIAQELLSTWPDLCKKHDIPCSAMHLSSGYTADEKTGNRYVFTMNKKRYPDFKAMVAHF
HKNGIKVVPNIKPYALQTHPHYKRLHSGDALFRDPAIDEPVVTRIWSAGVGDNEKGSWVD
MTSEEGRKWWAEGVRSLIEAGCDGMWDDNNEFYLHDDDFISASSFPHDISSPIEGKGSVG
LLGRMINTELMNYVSHTELEKANPERRTYVLTRSGNVGTFKYANSTWSGDNLTSWHCLRG
SQAIQINAGMSLMQSYGSDIGGFAGPLPTPEMFVRWVQLGVTHSRFCIHSFKPNKNDPSG
AAATNTPWMYPEMLPIIRNAIKWRYEHIPFFNNLMWLSHEEALPATAWLGYGPFASDPNL
YTQEILEGFDAWLGIGNILVCPALFEGQLTREVYFPKSSPEDNSVYFDLHAPFRQYLAGT
KAIIATPLEHFGLFAREGSVIPVGKEYATVTELKGPGRTTIDGVDTLLESEGGVVGLDDW
RGIKIFPSTNSKREYEGKWTEDDGISAKPKKTVVKVTYRGDKESVDVKVTFEQNEFKTLW
GDRVYVILPAGDERKVNGAKEVQKDGKTVWEVEVGKKSHWVIV

Enzyme Prediction     

No EC number prediction in I302_00774-t42_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	258	741	1.1e-89	0.9953161592505855

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
269885	GH31_glycosidase_Aec37	1.73e-144	277	621	1	319	E.coli Aec37-like. Glycosyl hydrolase family 31 (GH31) domain of a bacterial protein family represented by Escherichia coli protein Aec37. The gene encoding Aec37 (aec-37) is located within a genomic island (AGI-3) isolated from the extraintestinal avian pathogenic Escherichia coli strain BEN2908. The function of Aec37 and its orthologs is unknown; however, deletion of a region of the genome that includes aec-37 affects the assimilation of seven carbohydrates, decreases growth rate of the strain in minimal medium containing galacturonate or trehalose, and attenuates the virulence of E. coli BEN2908 in chickens. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
395838	Glyco_hydro_31	2.84e-88	258	741	1	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
224418	YicI	1.36e-86	127	835	115	741	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
269890	GH31_glucosidase_II_MalA	5.01e-56	316	641	34	338	Alpha-glucosidase II-like. Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This subgroup also includes the MalA alpha-glucosidase from Sulfolobus solfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
269889	GH31_GANC_GANAB_alpha	1.45e-44	317	742	35	427	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UOH80346.1|GH31	0.0	1	873	1	874
KGB78232.3|GH31	0.0	1	873	1	874
AUB27924.1|GH31	0.0	1	873	1	874
AFR97856.1|GH31	0.0	1	873	1	874
ADV20909.1|GH31	0.0	1	873	1	874

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DJW_A	8.08e-28	164	683	66	540	Crystal structure of Family 31 alpha-glucosidase (BT_3299) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5DJW_B Crystal structure of Family 31 alpha-glucosidase (BT_3299) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
4B9Y_A	1.38e-26	163	754	155	685	Crystal Structure of Apo Agd31B, alpha-transglucosylase in Glycoside Hydrolase Family 31 [Cellvibrio japonicus],4B9Z_A Crystal Structure of Agd31B, alpha-transglucosylase, complexed with Acarbose [Cellvibrio japonicus],4BA0_A Crystal Structure of Agd31B, alpha-transglucosylase, complexed with 5F-alpha-GlcF [Cellvibrio japonicus]
5I23_A	1.43e-26	163	754	132	662	Crystal Structure of Agd31B, alpha-transglucosylase in Glycoside Hydrolase Family 31, in complex with Cyclophellitol Aziridine probe CF022 [Cellvibrio japonicus Ueda107],5I24_A Crystal Structure of Agd31B, alpha-transglucosylase in Glycoside Hydrolase Family 31, in complex with Cyclophellitol Aziridine probe CF021 [Cellvibrio japonicus Ueda107],5NPB_A Crystal Structure of cjAgd31B (alpha-transglucosylase from Glycoside Hydrolase Family 31) in complex with alpha Cyclophellitol Cyclosulfate probe ME647 [Cellvibrio japonicus],5NPE_A Crystal Structure of cjAgd31B (alpha-transglucosylase from Glycoside Hydrolase Family 31) in complex with beta Cyclophellitol Aziridine probe KY358 [Cellvibrio japonicus Ueda107]
5NPC_A	5.70e-26	163	754	131	661	Crystal Structure of D412N nucleophile mutant cjAgd31B (alpha-transglucosylase from Glycoside Hydrolase Family 31) in complex with unreacted alpha Cyclophellitol Cyclosulfate probe ME647 [Cellvibrio japonicus],5NPD_A Crystal Structure of D412N nucleophile mutant cjAgd31B (alpha-transglucosylase from Glycoside Hydrolase Family 31) in complex with alpha Cyclophellitol Aziridine probe CF021 [Cellvibrio japonicus]
5JOU_A	4.50e-22	230	772	350	880	Bacteroides ovatus Xyloglucan PUL GH31 [Bacteroides ovatus],5JOV_A Bacteroides ovatus Xyloglucan PUL GH31 with bound 5FIdoF [Bacteroides ovatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9F234|AGL2_BACTQ	9.78e-45	157	759	145	691	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
sp|B3PEE6|OL4AG_CELJU	7.08e-26	163	754	155	685	Oligosaccharide 4-alpha-D-glucosyltransferase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=agd31B PE=1 SV=1
sp|P79403|GANAB_PIG	3.41e-25	158	742	263	811	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1
sp|Q9US55|GLU2A_SCHPO	4.39e-25	189	779	272	831	Glucosidase 2 subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gls2 PE=1 SV=1
sp|P38138|GLU2A_YEAST	4.55e-25	164	747	273	815	Glucosidase 2 subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROT2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999975	0.000102

TMHMM  Annotations     

There is no transmembrane helices in I302_00774-t42_1-p1.