CAZyme Information: HpaG811655:RNA-p1

Basic Information

• Species: Hyaloperonospora arabidopsidis
• Lineage: Oomycota; NA; ; Peronosporaceae; Hyaloperonospora; Hyaloperonospora arabidopsidis
• CAZyme ID: HpaG811655:RNA-p1
• CAZy Family: GT4
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
256		28242.22	4.3965

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HarabidopsidisEmoy2	14267	559515	285	13982

• Gene Location: location=HaraEmoy2_SC0165:77046-77816(-)

Full Sequence      

MKHAAAIASISLLGVTAADSCEMVESPIQQSTTSPIQQSTTSPNSPNFARFFDQNRFNTM
FPNAVELYNFDGLVTAADKYSEFANSGDETNDKRELAAFLAQTAHGSDNFKAAEEYARDT
YSVWQYCDNSTYPCAAGRRYHGRGPMQLAWNYNYYSAGKELGIDLLNNPDIVAQDTAVTW
MTALWFWMTPQKNGQIIHDFVAGVNGFARSTDTINGGLECGPDAPNQVNEQQRIAYFVKM
CEALDVQPLGNTSCNA

Enzyme Prediction     

EC	3.2.1.14:16

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH19	49	254	8.4e-63	0.974025974025974

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
381595	chitinase_GH19	2.11e-71	52	254	1	224	Glycoside hydrolase family 19, chitinase domain. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Glycoside hydrolase family 19 chitinases are found primarily in plants (classes I, III, and IV), but some are found in bacteria. Class I and II chitinases are similar in their catalytic domains. Class I chitinases have an N-terminal cysteine-rich, chitin-binding domain which is separated from the catalytic domain by a proline and glycine-rich hinge region. Class II chitinases lack both the chitin-binding domain and the hinge region. Class IV chitinases are similar to class I chitinases, but they are smaller in size due to certain deletions. Despite lacking any significant sequence homology with lysozymes, structural analysis reveals that family 19 chitinases, together with family 46 chitosanases, are similar to several lysozymes including those from T4-phage and from goose. The structures reveal that the different enzyme groups arose from a common ancestor glycohydrolase antecedent to the prokaryotic/eukaryotic divergence.
395130	Glyco_hydro_19	4.39e-54	52	254	7	232	Chitinase class I.
225720	COG3179	4.17e-11	54	218	6	187	Predicted chitinase [General function prediction only].
381610	chitinase-like	0.003	98	215	1	105	chitinase-like domain. This family includes proteins such as chitinases, chitosanase, pesticin, and endolysin, which are involved in the degradation of 1,4-N-acetyl D-glucosamine linkages in chitin polymers and related activities. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Chitosanase enzymes hydrolyze chitosan, a biopolymer of beta (1,4)-linked-D-glucosamine (GlcN) residues produced by partial or full deacetylation of chitin. Pesticin (Pst) is a anti-bacterial toxin produced by Yersinia pestis that acts through uptake by the target related bacteria and the hydrolysis of peptidoglycan in the periplasm. The dsDNA phages of eubacteria use endolysins or muralytic enzymes in conjunction with hollin, a small membrane protein, to degrade the peptidoglycan found in bacterial cell walls. Similarly, bacteria produce autolysins to facilitate the biosynthesis of its cell wall heteropolymer peptidoglycan and cell division.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ22871.1|GH19	4.14e-135	1	256	1	256
CCD28125.1|GH19	1.16e-126	47	256	52	260
AAN31509.1|GH19	5.22e-126	44	256	23	234
AVH63884.1|CBM50|GH19	1.22e-57	48	254	77	276
AIG55513.1|CBM1|GH19	2.60e-57	51	254	24	222

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3WH1_A	3.87e-54	57	254	13	206	Crystal Structure of a Family GH19 Chitinase from Bryum coronatum in complex with (GlcNAc)4 at 1.0 A resolution [Gemmabryum coronatum]
4IJ4_A	2.13e-53	57	254	12	205	Crystal Structure of a Family GH19 chitinase from Bryum coronatum in complex with (GlcNAc)4 [Gemmabryum coronatum]
7V91_A	7.07e-50	68	254	29	236	Chain A, GH19 Chitinase [Ficus microcarpa],7V91_B Chain B, GH19 Chitinase [Ficus microcarpa],7V91_C Chain C, GH19 Chitinase [Ficus microcarpa],7V91_D Chain D, GH19 Chitinase [Ficus microcarpa]
2CJL_A	1.20e-49	56	254	7	204	Crystal Structure And Enzymatic Properties Of A Bacterial Family 19 Chitinase Reveal Differences With Plant Enzymes [Streptomyces coelicolor],2CJL_B Crystal Structure And Enzymatic Properties Of A Bacterial Family 19 Chitinase Reveal Differences With Plant Enzymes [Streptomyces coelicolor]
7V92_A	1.58e-48	68	254	29	236	Chain A, GH19 Chitinase [Ficus microcarpa],7V92_B Chain B, GH19 Chitinase [Ficus microcarpa],7V92_C Chain C, GH19 Chitinase [Ficus microcarpa],7V92_D Chain D, GH19 Chitinase [Ficus microcarpa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P51613|CHIB_VITVI	2.37e-48	68	255	92	300	Basic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT1B PE=2 SV=1
sp|P86473|CHIT_ACTCC	8.12e-48	68	254	100	307	Endochitinase OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04863 PE=1 SV=2
sp|Q41596|CHI1_THECC	3.12e-47	68	254	99	306	Endochitinase 1 OS=Theobroma cacao OX=3641 GN=CHIA1 PE=2 SV=1
sp|P21226|CHI2_PEA	3.36e-47	68	254	95	301	Endochitinase A2 OS=Pisum sativum OX=3888 GN=CHI2 PE=1 SV=2
sp|Q39799|CHI1_GOSHI	2.62e-46	68	254	101	309	Endochitinase 1 OS=Gossypium hirsutum OX=3635 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.008381	0.991595	CS pos: 18-19. Pr: 0.7576

TMHMM  Annotations     

There is no transmembrane helices in HpaG811655:RNA-p1.