CAZyme Information: HVAS_10745690.1-p1

Basic Information

• Species: Hemileia vastatrix
• Lineage: Basidiomycota; Pucciniomycetes; ; Zaghouaniaceae; Hemileia; Hemileia vastatrix
• CAZyme ID: HVAS_10745690.1-p1
• CAZy Family: GT50
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
702		76240.18	4.6296

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HvastatrixRaceXXXIII	12854	N/A	284	12570

• Gene Location: location=PHNK01074569:2536-5189(+)

Full Sequence      

MPSYHPIPLPGSFPDPPIHTPIHPPSSATTSNSNSNSSTSTSTSTTFDPQSNPSNLYSYN
SIDPPSHPSAAPHHPPNPRSSTSTHTNCRVSTASKSRDAYADTYGVTASDSGPDISSLNA
IAAGLVGSDYARDSFISYDDGGAGCFPGGPGPPGSNGSLAAGLFYQFGATTENLLWKEEN
KEPDDYLHDPDPELDHLADRKFAIWSARGWLNIFTLGFLIAGLISLFALYPILTYVLHHA
PGYLGWNLGGINGTGQVPDIPGFPSLIDATTPESAKKRVGFDGKKYNLVFSDEFNTDNRT
FWPGDDPFWEAVDLNYWQTVNYEWYDPDAAVTRDGNLILTVSQEPLHNLNHRSAMIQSWN
KFCFTEGYIEVNVSLPGKANVQGLWPGIWTMGNLGRAGYGASNDGMWPYSYDACDVGTLP
NQTNADGSGPAAAKHSGSRDYGGELSWLPGQRTSACTCKGEDHPGPHVGVGRSGPEIDII
EAQYGYGPSGNLGSASQSIQIAPMDSGYNWRNDSSAMHIANPTTTSQSTNGTIINAWHGS
VYQESLSGITITDGQSYEGNGFQTFGYEYTRGYASDGGRITWSYNGQETWTIYADSIGAN
PDTGISARPITMEPMYIILNLGLSYATFIVLFFASGVFINFMFLPPCFFVGMAVVPLDIR
NKFQTIEWQKLNFPARMYIDYVRVYQREGQRPQIGCDPSGML

Enzyme Prediction     

No EC number prediction in HVAS_10745690.1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	292	626	1.5e-121	0.9263803680981595

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	179	700	6	462	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.54e-132	288	686	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	3.52e-35	265	700	28	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	8.70e-22	290	685	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	3.61e-06	292	404	1	102	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDR37048.1|GH16_2	1.09e-179	169	698	111	588
CCA67817.1|GH16_2	1.14e-172	150	699	103	598
CDR38565.1|GH16_2	9.83e-167	174	624	171	611
CED84082.1|GH16_2	4.06e-165	173	699	191	678
QRW15086.1|GH16_2	1.72e-157	170	698	164	641

PDB Hits     

HVAS_10745690.1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	4.45e-103	179	699	254	724	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	2.74e-102	179	699	214	672	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87023|KRE6_CANAX	8.68e-102	179	699	240	702	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	1.67e-100	162	699	225	699	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|O13941|YEPB_SCHPO	1.68e-100	173	700	123	589	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000047	0.000000

TMHMM  Annotations     

Start	End
211	233
617	639