CAZyme Information: HVAS_10150260.1-p1

Basic Information

• Species: Hemileia vastatrix
• Lineage: Basidiomycota; Pucciniomycetes; ; Zaghouaniaceae; Hemileia; Hemileia vastatrix
• CAZyme ID: HVAS_10150260.1-p1
• CAZy Family: GH32
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
701		79718.69	7.6484

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HvastatrixRaceXXXIII	12854	N/A	284	12570

• Gene Location: location=PHNK01015026:10886-14324(+)

Full Sequence      

MILIDGLDLSGFKFGPSWSNHWVRATLRVPPAWVDKERVQFEFDPGCEALIYTTEGLPLQ
GITGGFDGSRRVEFIIPPEKRSFPLRIYIEVSANAMFGMPFDKGVKDDVYFTLQSADIVV
PRQEAWHLMWDFNAILGKFNFQMFFLICAYITKNIPTNQPLSLKAQSVAMDISNQFNASD
LSTIEKCRKIAEKVFGTGWRERNVYDQYHDWSTSGDESVPVLALGNCHIDTAWLWPFSVT
QQKIARSWSTQVDLIERYPEFRFVASQGQQFKWLENLYPQLFQKVRSQIEDGKFQFLGAT
WVENDTNMPSGEALCRQFSFGQRYFKSRFGKTSEVFWLPDSFGYSSQLPQICRLAGVPYF
FTQKLSWSQFNKFPHTSFNWIGIDGTQMLAHMTPVETYTAQANVGDVVKAINNHKDLEWC
DKSMLVYGNGDGGGGPLPPMIESLRRIRAAANNSPGSSLPKVTQGRVIPEFYEMILNKTR
NGMDLPSWRGEYMSTQNKSWIKYGKMASLNSFARTFLLRKLKFHDVLPGSAIAKVYVDAE
RIYADIAKKGQDLLNEALNALIPNQDSESTSIFGLNTLSFPRQEVVEISYKNHDKNSAFS
ASQINQETGYIILKDDKNYERLSQPVSLTQYNNDFHPATASTTIDEMGTLFYILANRFLS
VRISSRGRIVSIVDSALGRKVICNVFNAGASWFPKENLLDL

Enzyme Prediction     

EC	3.2.1.24:32

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH38	221	477	2.6e-77	0.929368029739777

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
212123	GH38N_AMII_ScAms1_like	3.24e-162	220	478	1	258	N-terminal catalytic domain of yeast vacuolar alpha-mannosidases and similar proteins; glycoside hydrolase family 38 (GH38). The family is represented by Saccharomyces cerevisiae alpha-mannosidase (Ams1) and its eukaryotic homologs. Ams1 functions as a second resident vacuolar hydrolase in S. cerevisiae. It aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing alpha-d-mannose residues. Ams1 forms an oligomer in the cytoplasm and retains its oligomeric form during the import process. It utilizes both the Cvt (nutrient-rich conditions) and autophagic (starvation conditions) pathways for biosynthetic delivery to the vacuole. Mutants in either pathway are defective in Ams1 import. Members in this family show high sequence similarity with rat ER/cytosolic alpha-mannosidase Man2C1.
212101	GH38N_AMII_ER_cytosolic	2.75e-108	221	473	2	247	N-terminal catalytic domain of endoplasmic reticulum(ER)/cytosolic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The subfamily is represented by Saccharomyces cerevisiae vacuolar alpha-mannosidase Ams1, rat ER/cytosolic alpha-mannosidase Man2C1, and similar proteins. Members in this family share high sequence similarity. None of them have any classical signal sequence or membrane spanning domains, which are typical of sorting or targeting signals. Ams1 functions as a second resident vacuolar hydrolase in S. cerevisiae. It aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing alpha-d-mannose residues. Ams1 utilizes both the cytoplasm to vacuole targeting (Cvt, nutrient-rich conditions) and autophagic (starvation conditions) pathways for biosynthetic delivery to the vacuole. Man2C1is involved in oligosaccharide catabolism in both the ER and cytosol. It can catalyze the cobalt-dependent cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl-enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst.
212124	GH38N_AMII_Man2C1	4.13e-99	221	462	2	237	N-terminal catalytic domain of mammalian cytosolic alpha-mannosidase Man2C1 and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily corresponds to cytosolic alpha-mannosidase Man2C1 (also known as ER-mannosidase II or neutral/cytosolic mannosidase), mainly found in various vertebrates, and similar proteins. Man2C1 plays an essential role in the catabolism of cytosolic free oligomannosides derived from dolichol intermediates and the degradation of newly synthesized glycoproteins in ER or cytosol. It can catalyze the cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Man2C1 is a cobalt-dependent enzyme belonging to alpha-mannosidase class II. It has a neutral pH optimum and is strongly inhitibed by furanose analogs swainsonine (SW) and 1,4-dideoxy-1,4-imino-D-mannitol (DIM), moderately by deoxymannojirimycin (DMM), but not by kifunensine (KIF). DMM and KIF, both pyranose analogs, are normally known to inhibit class I alpha-mannosidase.
395852	Glyco_hydro_38	5.62e-85	221	449	2	236	Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.
223460	AMS1	2.16e-77	8	593	43	576	Alpha-mannosidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRW26492.1|GH38	9.56e-204	11	682	148	837
QRW14078.1|GH38	6.38e-201	23	696	99	789
QRV85501.1|GH38	5.33e-199	11	696	103	839
CDR38773.1|GH38	1.20e-193	11	698	88	815
CDI51481.1|GH38	9.17e-190	13	689	93	790

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6LZ1_A	1.87e-143	5	682	88	750	Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_B Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_C Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_D Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-]
7DD9_A	4.26e-140	5	682	88	750	Chain A, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_C Chain C, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_E Chain E, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_G Chain G, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct]
5JM0_A	6.81e-129	14	617	114	726	Structure of the S. cerevisiae alpha-mannosidase 1 [Saccharomyces cerevisiae S288C]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9UT61|MAN1_SCHPO	2.81e-143	5	682	88	750	Alpha-mannosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ams1 PE=1 SV=1
sp|P22855|MAN1_YEAST	2.69e-128	14	617	101	713	Alpha-mannosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AMS1 PE=1 SV=2
sp|Q54K67|MANG_DICDI	2.17e-118	11	682	72	764	Alpha-mannosidase G OS=Dictyostelium discoideum OX=44689 GN=manG PE=1 SV=1
sp|Q9NTJ4|MA2C1_HUMAN	4.21e-92	11	682	70	717	Alpha-mannosidase 2C1 OS=Homo sapiens OX=9606 GN=MAN2C1 PE=1 SV=1
sp|Q91W89|MA2C1_MOUSE	4.04e-91	11	682	69	716	Alpha-mannosidase 2C1 OS=Mus musculus OX=10090 GN=Man2c1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000058	0.000000

TMHMM  Annotations     

There is no transmembrane helices in HVAS_10150260.1-p1.