CAZyme Information: HMPREF1544_08949-t46_1-p1

Basic Information

• Species: Mucor circinelloides
• Lineage: Mucoromycota; Mucoromycetes; ; Mucoraceae; Mucor; Mucor circinelloides
• CAZyme ID: HMPREF1544_08949-t46_1-p1
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
605	KE124043|CGC1	67906.28	4.4185

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mcircinelloides1006PhL	12410	1220926	183	12227

• Gene Location: location=KE124043:42950-45166(+)

Full Sequence      

MRLFSSFFIAGITSLITAAPTSLKRRQLTTTVTLVNQCSNLIHVGQLGDTVLNSGNPINI
VSGSSETYQFDGQWSGRFWARENCQANDCQIAGSTYPASLAEFTFNDDSGKDYYDISFVD
GYNLPISIEPIQPDMSALQQGGRYWCGAPSCSAVPSCSAELQLLSADGAYVGCKSACSQF
GNSEYCCSGVNDTPETCPINEYAAAVKDACPDVYAYAYDDPKRDILIDGKLFNKMSAIGI
ETNTIGGGLLECWKNKVAQKEHIIYFVVHTQMIDQKDIDSVFIKYVKEYQDINGSYVRVY
EKPPTALEFLRGSVQPNRPAVIKGGFDHWPARDGWSNEYLRSKMGDTIVTVAATPNGYAD
AVTFDPETQKEYFTMPYEDRMTFNKFLDLVEGKQVSENANYISLQNGSLPVEYSAIESDV
DKDIDWCSEALGKKPDAVNFWFGDDKSATSLHKDPYENCYAVIRGQKTFILYPPTEYYCM
HESVYQNAIYEPNPTTNKLELKPIDGTTPWIPVNPLYPDFTKFPRFKHACPVTVTVNEGD
MLYLPALWFHQVMQKGEQGVIAINYWYDMDYSNMLYPTMALYRRLLTGVMENNRELLDND
GSDIE

Enzyme Prediction     

No EC number prediction in HMPREF1544_08949-t46_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH152	36	225	6.9e-42	0.9305555555555556

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
404504	Cupin_8	1.88e-81	306	573	1	251	Cupin-like domain. This cupin like domain shares similarity to the JmjC domain.
395248	Thaumatin	7.48e-61	62	221	28	193	Thaumatin family.
185757	TLP-PA	3.02e-59	31	222	1	203	allergenic/antifungal thaumatin-like proteins: plant and animal homologs. This subfamily is represented by the thaumatin-like proteins (TLPs), Cherry Allergen Pru Av 2 TLP, Peach PpAZ44 TLP (a propylene-induced TLP in abscission), the Caenorhabditis elegans thaumatin family member (thn-6), and other plant and animal homologs. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Due to their inducible expression by environmental stresses such as pathogen/pest attack, drought and cold, plant TLPs are classified as the pathogenesis-related (PR) protein family 5 (PR5). Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. TLPs within this subfamily contain 16 conserved Cys residues.
128501	THN	2.12e-45	33	221	2	200	Thaumatin family. The thaumatin family gathers proteins related to plant pathogenesis. The thaumatin family includes very basic members with extracellular and vacuolar localization. Thaumatin itsel is a potent sweet-tasting protein. Several members of this family display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism.
185758	TLP-F	2.57e-28	32	226	1	212	thaumatin-like proteins: basidiomycete homologs. This subfamily is represented by Lentinula edodes TLG1, a thaumatin-like protein (TLP), as well as, other basidiomycete homologs. In general, TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. TLG1 TLP is involved in lentinan degradation and fruiting body senescence. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited beta-1,3-glucanase activity and demonstrated lentinan degrading activity. TLG1 is proposed to be involved in lentinan and cell wall degradation during senescence following harvest and spore diffusion. TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. TLG1 from Lentinula edodes contains the required acidic amino acids conserved in the appropriate positions to possess an electronegative cleft. TLPs within this subfamily contain 13 conserved Cys residues; the number of total Cys residues in these TLPs varies from 16 in L. edodes TLG1 to 18 in other basidiomycete homologs.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB4061803.1|GT65	6.31e-49	334	586	424	671
CAF2891108.1|GT65	6.31e-49	334	586	424	671
QHO26056.1|GH152	4.50e-41	32	229	24	231
ACT11052.1|GH152	2.21e-39	32	229	24	231
BAT95405.1|GH152	2.33e-39	32	220	25	224

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QU2_A	3.11e-77	290	572	23	299	The structure of JMJD7 with alpha-KG [Mus musculus],4QU2_B The structure of JMJD7 with alpha-KG [Mus musculus]
5NFO_A	2.19e-75	301	572	52	316	Human JMJD7 in complex with Mn and 2OG in the P21212 form [Homo sapiens],5NFO_B Human JMJD7 in complex with Mn and 2OG in the P21212 form [Homo sapiens]
5NFN_A	2.19e-75	301	572	52	316	JMJD7 IN COMPLEX WITH MN AND 2OG IN THE H32 FORM [Homo sapiens],5NFN_B JMJD7 IN COMPLEX WITH MN AND 2OG IN THE H32 FORM [Homo sapiens],5NFN_C JMJD7 IN COMPLEX WITH MN AND 2OG IN THE H32 FORM [Homo sapiens],5NFN_D JMJD7 IN COMPLEX WITH MN AND 2OG IN THE H32 FORM [Homo sapiens]
4QSZ_A	3.46e-73	290	572	392	668	Crystal structure of mouse JMJd7 fused with maltose-binding protein [Mus musculus],4QSZ_B Crystal structure of mouse JMJd7 fused with maltose-binding protein [Mus musculus]
7YXJ_A	6.86e-67	270	573	2	305	Chain A, GH14974p [Drosophila melanogaster],7YXJ_B Chain B, GH14974p [Drosophila melanogaster],7YXJ_C Chain C, GH14974p [Drosophila melanogaster],7YXJ_D Chain D, GH14974p [Drosophila melanogaster],7YXK_A Chain A, GH14974p [Drosophila melanogaster],7YXK_B Chain B, GH14974p [Drosophila melanogaster],7YXL_A Chain A, GH14974p [Drosophila melanogaster],7YXL_B Chain B, GH14974p [Drosophila melanogaster]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P0C872|JMJD7_MOUSE	1.50e-76	290	572	21	297	Bifunctional peptidase and (3S)-lysyl hydroxylase Jmjd7 OS=Mus musculus OX=10090 GN=Jmjd7 PE=1 SV=1
sp|P0C870|JMJD7_HUMAN	6.32e-75	301	572	33	297	Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7 OS=Homo sapiens OX=9606 GN=JMJD7 PE=1 SV=1
sp|Q54CS7|JMJCE_DICDI	1.45e-61	277	576	9	333	JmjC domain-containing protein E OS=Dictyostelium discoideum OX=44689 GN=jcdE PE=4 SV=2
sp|P83336|TP1B_MALDO	8.44e-39	60	229	23	202	Thaumatin-like protein 1b (Fragment) OS=Malus domestica OX=3750 PE=2 SV=1
sp|Q9FSG7|TP1A_MALDO	1.14e-38	32	229	27	236	Thaumatin-like protein 1a OS=Malus domestica OX=3750 GN=TL1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000255	0.999716	CS pos: 26-27. Pr: 0.9202

TMHMM  Annotations     

There is no transmembrane helices in HMPREF1544_08949-t46_1-p1.