CAZyme Information: HMPREF1544_03836-t46_1-p1

Basic Information

• Species: Mucor circinelloides
• Lineage: Mucoromycota; Mucoromycetes; ; Mucoraceae; Mucor; Mucor circinelloides
• CAZyme ID: HMPREF1544_03836-t46_1-p1
• CAZy Family: GH17
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
511	KE123936|CGC1	54604.16	5.4880

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mcircinelloides1006PhL	12410	1220926	183	12227

• Gene Location: location=KE123936:66534-68308(+)

Full Sequence      

MRLAGVLAKIACGVLLLLNGASSAPTNSLQSRAAGSNKVIVGQDYLPTKIDFKKYTHIYY
AFALQVEGNVPVWEDPEIMNEDVEYGFPALVKAAKAAGTKVTISVGGWSGSTQFSPMAAS
AANRAAWIKWNVDLVTKYDTAGVDIDWEYPSASGPGCNTKAWNDVDNLLLLVKELRVALD
AASPTNYKEITMAVRIIPWGGETDATDVSAFVPYVDRFHVMTFDVNGAWNSISGPNSPFR
VESGKGFQKGFVEGIEYWKSAGVPYNKLAGGVAFYGRAQTLTVTSNPTTQYNPAISPNPP
LGDSDDGPWTNKWCSIDSSPASGQWKYRSLRSQGVLTSPTVAASPWIRNFDNITQTPWLY
NPTNKQFISYDDPVSIGVKTQWAVSKDLAGLFVWSLDQDNGELIAAMQPVIGGNPPPTST
TSTVGPTSTSTSTSTVTTTTTVSTKTTTTATSTASPAACSGIAIYNASTAYNGGAKVTYN
GGIYTANWWTQGETPSPTAPAWAAWKYVGPC

Enzyme Prediction     

No EC number prediction in HMPREF1544_03836-t46_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	39	402	4.6e-65	0.8817567567567568

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
214753	Glyco_18	8.97e-71	40	399	11	334	Glyco_18 domain.
119365	GH18_chitinase	4.52e-66	40	399	10	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
225862	ChiA	5.87e-62	42	415	52	440	Chitinase, GH18 family [Carbohydrate transport and metabolism].
395573	Glyco_hydro_18	5.06e-56	50	399	18	307	Glycosyl hydrolases family 18.
119351	GH18_chitolectin_chitotriosidase	1.79e-53	45	399	18	341	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMP42675.1|GH18	6.10e-130	10	414	7	421
AJD76187.1|GH18	1.44e-126	38	404	2	378
CDS13735.1|GH18	1.48e-113	26	404	16	402
CDS06898.1|GH18	9.86e-108	33	415	22	416
ATY63066.1|GH18	6.60e-82	50	399	38	381

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XWF_A	6.68e-45	46	410	13	368	Chain A, Fungal chitinase from Rhizomucor miehei (SeMet-substituted proteins) [Rhizomucor miehei]
3WL1_A	2.45e-41	56	400	37	360	Crystal structure of Ostrinia furnacalis Group I chitinase catalytic domain in complex with reaction products (GlcNAc)2,3 [Ostrinia furnacalis],3WQV_A Crystal structure of Ostrinia furnacalis Group I chitinase catalytic domain in complex with a(GlcN)5 [Ostrinia furnacalis],3WQW_A Crystal structure of Ostrinia furnacalis Group I chitinase catalytic domain in complex with a(GlcN)6 [Ostrinia furnacalis]
3WKZ_A	6.49e-41	56	400	37	360	Crystal Structure of the Ostrinia furnacalis Group I Chitinase catalytic domain E148Q mutant [Ostrinia furnacalis]
3WL0_A	1.72e-40	56	400	37	360	Crystal structure of Ostrinia furnacalis Group I chitinase catalytic domain E148A mutant in complex with a(GlcNAc)2 [Ostrinia furnacalis]
3W4R_A	3.36e-40	56	400	55	378	Crystal structure of an insect chitinase from the Asian corn borer, Ostrinia furnacalis [Ostrinia furnacalis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P20533|CHIA1_NIACI	6.62e-39	8	422	14	464	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1
sp|P36362|CHIT_MANSE	1.93e-37	50	400	47	376	Endochitinase OS=Manduca sexta OX=7130 PE=2 SV=1
sp|A5FB63|CHIA_FLAJ1	5.21e-29	40	399	36	446	Chitinase ChiA OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=chiA PE=1 SV=1
sp|Q95M17|CHIA_BOVIN	1.38e-26	45	400	41	366	Acidic mammalian chitinase OS=Bos taurus OX=9913 GN=CHIA PE=1 SV=1
sp|Q13231|CHIT1_HUMAN	1.77e-26	45	400	41	364	Chitotriosidase-1 OS=Homo sapiens OX=9606 GN=CHIT1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000547	0.999417	CS pos: 23-24. Pr: 0.9636

TMHMM  Annotations     

There is no transmembrane helices in HMPREF1544_03836-t46_1-p1.