CAZyme Information: HMPREF1544_03123-t46_1-p1

Basic Information

• Species: Mucor circinelloides
• Lineage: Mucoromycota; Mucoromycetes; ; Mucoraceae; Mucor; Mucor circinelloides
• CAZyme ID: HMPREF1544_03123-t46_1-p1
• CAZy Family: GH15
• CAZyme Description: chitin synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
831		95246.27	8.4997

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mcircinelloides1006PhL	12410	1220926	183	12227

• Gene Location: location=KE123926:38096-40920(-)

Full Sequence      

MEGSPYYPSQLHPQQQIPPRIPPHYMMSSSSPHLLMSPQPLNHNSFPPSSNHSSQHHIYP
AFYPHAQSQPPQPPSAQPEFQYNPAPPHHQVRQVRRYKTIKRLVQIPQGKLVLNCPVPIQ
YLERVPLRDPKEFNMMRYTAITCDANEFHQKNYTLRQEIMNRETEIAICITMYNEDEILL
SRTLHGIMKNLTQLTKRYRSPIWGEHAWQKVVICIIADGREKIHPRVLDMLSVLGIYQEG
IAKSTVNEKPVHAHLYEYTSQLSLTPDLTYKGAINDIVPAQIIFCLKEKNQRKINSHRWF
FQAICPVLQPNVCILMDVGTRPGPHSIYKLWLPFEKHRDIGGACGEVRAMTGKGNVYLLN
PLVAAQNFEYKISNILDKPLESVLGYIQVLPGAFSAYRYAAIQNDMTGEGPLKKYFLGDT
SGGDVSEGIFEANMYLAEDRILCYELVAKAQSQWRLYYVSGAYGETDVPFSVAEFISQRR
RWLNGSFFAGVYSLVHWGKILNTNHTTGRKCLLIIELVYLCINWLFSWFALGNFFLSFYI
LTGSLAVMQQPPFSAEAADIVHTVLTYIYAVLIICLFLIAMGNRPQGSQGSYGLIMIFFS
LLMIYVIFSSAWIVYVGISTELSKPQSISSLLSNGPFRDIIISMASTFGVFILSGIIFLD
PWHMLTSFVQYFLLTPSYINILNTYAFCNTHDVSWGTKDIFENIPYLGHVKTKADQTTAE
ILLPEQKDVGYLYEESFKNLQKKESKNNKKYVDPKTRQEDYYRNFRTRLVVCWIFSNLIL
VIVICTMDNLGKLGNFDERTNTYMSFILWSVAALSVFRFIGSCVYRVLSFF

Enzyme Prediction     

EC	2.4.1.16:26	2.4.1.16:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	172	332	9.5e-74	0.9815950920245399

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396286	Chitin_synth_1	1.79e-104	172	334	1	163	Chitin synthase. This region is found commonly in chitin synthases classes I, II and III. Chitin a linear homopolymer of GlcNAc residues, it is an important component of the cell wall of fungi and is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases.
133033	Chitin_synth_C	2.27e-89	168	489	1	244	C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin. Chitin synthase, also called UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of GlcNAc residues formed by covalent beta-1,4 linkages. Chitin is an important component of the cell wall of fungi and bacteria and it is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases. Studies with fungi have revealed that most of them contain more than one chitin synthase gene. At least five subclasses of chitin synthases have been identified.
400626	Chitin_synth_1N	3.06e-30	104	171	2	70	Chitin synthase N-terminal. This is the N-terminal domain of Chitin synthase (pfam01644).
224136	BcsA	3.85e-26	283	615	115	408	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
133045	CESA_like	1.46e-15	273	409	46	179	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDS10378.1|GT2	0.0	92	831	69	806
BAA19857.1|GT2|2.4.1.16	5.59e-307	78	831	85	839
CDS12882.1|GT2	2.49e-304	92	831	82	824
CDS05079.1|GT2	3.06e-302	92	831	120	862
CDS03137.1|GT2	9.69e-301	92	831	88	831

PDB Hits     

HMPREF1544_03123-t46_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87073|CHS1_PHYB8	9.93e-308	78	831	85	839	Chitin synthase 1 OS=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) OX=763407 GN=chs1 PE=3 SV=1
sp|P30594|CHS1_RHIOL	5.74e-296	92	831	110	856	Chitin synthase 1 OS=Rhizopus oligosporus OX=4847 GN=CHS1 PE=3 SV=1
sp|P30595|CHS2_RHIOL	8.64e-293	92	831	112	856	Chitin synthase 2 OS=Rhizopus oligosporus OX=4847 GN=CHS2 PE=3 SV=1
sp|Q12632|CHS1_MUCCL	4.83e-262	90	831	94	849	Chitin synthase 1 OS=Mucor circinelloides f. lusitanicus OX=29924 GN=CHS1 PE=3 SV=1
sp|P30597|CHS1_SCHPO	1.97e-260	94	825	117	850	Chitin synthase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=chs1 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000085	0.000000

TMHMM  Annotations     

Start	End
525	547
560	582
597	619
640	662
667	689
769	791
806	828