CAZyme Information: HMPREF1544_00491-t46_1-p1

Basic Information

• Species: Mucor circinelloides
• Lineage: Mucoromycota; Mucoromycetes; ; Mucoraceae; Mucor; Mucor circinelloides
• CAZyme ID: HMPREF1544_00491-t46_1-p1
• CAZy Family: AA3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1994	KE123898|CGC2	225978.87	5.7262

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Mcircinelloides1006PhL	12410	1220926	183	12227

• Gene Location: location=KE123898:365698-371853(+)

Full Sequence      

MQQEDSRTDLVSLYQLGGQPPSEEAISNLLANRYKRHLPYTQLGYANLVVVNPYQPLELL
NDATLRSYADIGYKDLSEKKPALQPHVYELATKVYFNMRRTGEDQSIILSGITGSGKSTT
RTHFLCELLQLSTHNKRENKLGSQIQNALVVIEAFGHARTTQNVSASKFGMFQEIQFNER
GRILGTKTLTYQFDKSRVTQVPPNERTYHVFYSLLAGTTVDEKNALHINFRPDYFHYLNQ
SGCISTSDWNDEIEFSDLKSALKMCGFKAKTVTQIFQLLAAILHIGNLQFQENTDALAQE
ACIVKNTDILDIVAVMLGVSPSKLETCLTYKLKLIGKEVCTIFLNPQAAMEQRDELANAL
YNVLFMWIIESVNRKICYSGAQEPCNVIGIMDQFGFQNFATNGFQEFCVNLANERMHHFL
VNEHFGNEHGMNAAMMQDGVPLPMVKVVDNSACLELLLGKDKRQLIEDARNKTQALSKSS
SLGLGGITGLMDRDTARLQSGATDASNANFLAALQRQFSSHPSLSRGSQTYAFGINHFSG
TVHYSVDMFIEKNLDDLSPDFVNMLRDSSSNAFLSNLFQGTAMATESHPKDARTIVKAQL
SSKPTRAPTMRRRTTKRRPEKAAAANDTPASPEKEDMELDKQIKQAEEAYQDMQEMTVTD
QLYITLRDITFSMLDTRIYNIIHIRPNDIQAPEFDHNRIRSQIRAFLIPDLIVRHHFDFA
NYYTFEEFMERYQIFMQSLQLEASGSDKEHIERACALMNWNQQTVFVGREMVWLNYEVWK
DLEDGLRAAEKEERNRAKMELENNAAINTMSQPASEEDGYSNQQGDRLLTNPYSYDDDIN
QRYVDSPEQNYADNGSYAYSEGKRADASQWGEESEWGISGLAEGFGPNMDMSKMIEDIQA
PKIENIEEVPITSVRRWWVRFVWLNTFWIPSFLLSKLGKMKRSDIQMAWREKVTLCMIIF
WFSAVILFFIVGLGEVVCPGTSTMYSTSTVGNHNSANDNYMSVRGTVYDMTQFSKATHGT
SAYTATQEAMDALAGMDVSSSIPPPLTVACSGLVTDSNIKILANTSVNPQASFIHYSGDQ
VQITTLDDTKDPYWYWKTFLPTIQDYKKGTLVHLIKDLKADAESWGRYALAINNNVYDLN
DYFTTINTYGTASGNYSFLSGTVEDLFSLFKGTDATAKWEQYKGKMSVAEQDMNMNCLNN
YFLIGYVDPRESARCMFTNYLLLACACLMCLVILVKFLAALQFGSAPTPEDHDKFVICQI
PCYTEDEESLKKTIDSLTVMKYDDKRKLLFIIADGMVMGSGNDRPTPRIVLDILGYDTKQ
DPEPLMFKSVGEGAKQLNYGKIYSGLYECEGHVVPYLVVAKVGKASERAKPGNRGKRDSQ
MICMNFLNRVHFDSEMTPMELEMYHQIKNVIGVNPSFYEYILMVDSDTEVMDYSLNYLIS
SMLHDARIMGICGETALANEKQSWTTMIQVYEYYISHYLAKAFESLFGSVTCLPGCFCMY
RVRTPVKNEPLIIAPSVVHDYAENHVDTLHKKNLLSLGEDRYLTTLMMKHFPQYKMKFTP
YAMCRTVAPDKWKVLLSQRRRWINSTIHNLMEVALLPDLCGFCCFSMRFVVLVDLIGTLT
LPVSFAYLVYLIYVMAAHAGPFPTLAIGMLAGLYALQVIIFILKRQWQHIGWMIFYLFAL
PVYSFALPIYSFWHFDDFSWGNTRVVVGDSQRKIIVTDEEKFDEKMIPMKKWSVYEQELW
EMQSHRSGESGLTGNTYNSYGMVPPGYERSVYSGMSTPAGEYDYYRDTKVSDKPRNRQPY
AFTTSGSVVGSDYGGRAPTPGGMIPMQEYSRSMMATPPPGMPTRPVSFASSAHPDFMSQM
QPMQPMMMDPNYPPPRDMQSPGLYNTRVPLMSPATLDPNGTMGPQLFGHSQSELDMPTMG
NVSQMSVLPPMIPAGFPSDEEILSEIRAILATANLMSITKKQVREQLSDMFGFDMNFKKD
FINSSIEYILQGKL

Enzyme Prediction     

EC	2.4.1.16:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	1233	1738	1.8e-232	0.9924098671726755

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
276845	MYSc_Myo17	0.0	21	784	1	647	class XVII myosin, motor domain. This fungal myosin which is also known as chitin synthase uses its motor domain to tether its vesicular cargo to peripheral actin. It works in opposition to dynein, contributing to the retention of Mcs1 vesicles at the site of cell growth and increasing vesicle fusion necessary for polarized growth. Class 17 myosins consist of a N-terminal myosin motor domain with Cyt-b5, chitin synthase 2, and a DEK_C domains at it C-terminus. The chitin synthase region contains several transmembrane domains by which myosin 17 is thought to bind secretory vesicles. The catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Myosins are actin-dependent molecular motors that play important roles in muscle contraction, cell motility, and organelle transport. The head domain is a molecular motor, which utilizes ATP hydrolysis to generate directed movement toward the plus end along actin filaments. A cyclical interaction between myosin and actin provides the driving force. Rates of ATP hydrolysis and consequently the speed of movement along actin filaments vary widely, from about 0.04 micrometer per second for myosin I to 4.5 micrometer per second for myosin II in skeletal muscle. Myosin II moves in discrete steps about 5-10 nm long and generates 1-5 piconewtons of force. Upon ATP binding, the myosin head dissociates from an actin filament. ATP hydrolysis causes the head to pivot and associate with a new actin subunit. The release of Pi causes the head to pivot and move the filament (power stroke). Release of ADP completes the cycle. CyMoBase classifications were used to confirm and identify the myosins in this hierarchy.
367353	Chitin_synth_2	0.0	1230	1738	1	527	Chitin synthase. Members of this family are fungal chitin synthase EC:2.4.1.16 enzymes. They catalyze chitin synthesis as follows: UDP-N-acetyl-D-glucosamine + {(1,4)-(N-acetyl-beta-D-glucosaminyl)}(N) <=> UDP + {(1,4)-(N-acetyl-beta-D-glucosaminyl)}(N+1).
276950	MYSc	3.36e-146	24	774	1	632	Myosin motor domain superfamily. Myosin motor domain. The catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Myosins are actin-dependent molecular motors that play important roles in muscle contraction, cell motility, and organelle transport. The head domain is a molecular motor, which utilizes ATP hydrolysis to generate directed movement toward the plus end along actin filaments. A cyclical interaction between myosin and actin provides the driving force. Rates of ATP hydrolysis and consequently the speed of movement along actin filaments vary widely, from about 0.04 micrometer per second for myosin I to 4.5 micrometer per second for myosin II in skeletal muscle. Myosin II moves in discrete steps about 5-10 nm long and generates 1-5 piconewtons of force. Upon ATP binding, the myosin head dissociates from an actin filament. ATP hydrolysis causes the head to pivot and associate with a new actin subunit. The release of Pi causes the head to pivot and move the filament (power stroke). Release of ADP completes the cycle. CyMoBase classifications were used to confirm and identify the myosins in this hierarchy.
214580	MYSc	4.40e-144	9	784	10	674	Myosin. Large ATPases. ATPase; molecular motor. Muscle contraction consists of a cyclical interaction between myosin and actin. The core of the myosin structure is similar in fold to that of kinesin.
276849	MYSc_Myo22	1.12e-113	24	605	1	542	class XXII myosin, motor domain. These myosins possess an extended neck with multiple IQ motifs such as found in class V, VIII, XI, and XIII myosins. These myosins are defined by two tandem MyTH4 and FERM domains. The apicomplexan, but not diatom myosins contain 4-6 WD40 repeats near the end of the C-terminal tail which suggests a possible function of these myosins in signal transduction and transcriptional regulation. The catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Myosins are actin-dependent molecular motors that play important roles in muscle contraction, cell motility, and organelle transport. The head domain is a molecular motor, which utilizes ATP hydrolysis to generate directed movement toward the plus end along actin filaments. A cyclical interaction between myosin and actin provides the driving force. Rates of ATP hydrolysis and consequently the speed of movement along actin filaments vary widely, from about 0.04 micrometer per second for myosin I to 4.5 micrometer per second for myosin II in skeletal muscle. Myosin II moves in discrete steps about 5-10 nm long and generates 1-5 piconewtons of force. Upon ATP binding, the myosin head dissociates from an actin filament. ATP hydrolysis causes the head to pivot and associate with a new actin subunit. The release of Pi causes the head to pivot and move the filament (power stroke). Release of ADP completes the cycle. CyMoBase classifications were used to confirm and identify the myosins in this hierarchy.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDS03648.1|GT2	0.0	3	1760	2	1760
CDS05327.1|GT2	0.0	3	1829	2	1818
CDS05755.1|GT2	0.0	26	1990	23	1832
QDK64620.1|GT2	0.0	8	1990	8	1969
AZQ26804.1|GT2	0.0	8	1990	8	1969

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3MKD_A	2.79e-74	22	585	97	618	Crystal structure of myosin-2 dictyostelium discoideum motor domain S456Y mutant in complex with adp-orthovanadate [Dictyostelium discoideum]
1LVK_A	4.63e-74	22	585	98	619	X-Ray Crystal Structure Of The Mg (Dot) 2'(3')-O-(N- Methylanthraniloyl) Nucleotide Bound To Dictyostelium Discoideum Myosin Motor Domain [Dictyostelium discoideum]
1MMG_A	6.24e-74	22	585	98	619	X-Ray Structures Of The Mgadp, Mgatpgammas, And Mgamppnp Complexes Of The Dictyostelium Discoideum Myosin Motor Domain [Dictyostelium discoideum]
1MMD_A	6.24e-74	22	585	98	619	Truncated Head Of Myosin From Dictyostelium Discoideum Complexed With Mgadp-Bef3 [Dictyostelium discoideum],1MND_A Truncated Head Of Myosin From Dictyostelium Discoideum Complexed With Mgadp-Alf4 [Dictyostelium discoideum],1MNE_A Truncated Head Of Myosin From Dictyostelium Discoideum Complexed With Mg-Pyrophosphate [Dictyostelium discoideum],1VOM_A Complex Between Dictyostelium Myosin And Mgadp And Vanadate At 1.9a Resolution [Dictyostelium discoideum]
1MMN_A	6.24e-74	22	585	98	619	X-Ray Structures Of The Mgadp, Mgatpgammas, And Mgamppnp Complexes Of The Dictyostelium Discoideum Myosin Motor Domain [Dictyostelium discoideum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4P9K9|CHS8_USTMA	0.0	9	1990	6	2002	Chitin synthase 8 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CHS8 PE=3 SV=1
sp|J9VNT4|CHS5_CRYNH	0.0	8	1990	14	1929	Chitin synthase 5 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS5 PE=2 SV=1
sp|O13395|CHS6_USTMA	0.0	896	1992	51	1176	Chitin synthase 6 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CHS6 PE=3 SV=2
sp|J9VF17|CHS4_CRYNH	2.24e-309	885	1990	135	1268	Chitin synthase 4 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS4 PE=2 SV=2
sp|O13353|CHS4_MAGO7	1.19e-140	1257	1765	686	1215	Chitin synthase 4 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=CHS4 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000038	0.000000

TMHMM  Annotations     

Start	End
917	934
955	977
1216	1238
1609	1631
1641	1663
1670	1692