CAZyme Information: HMPREF1541_01691-t46_1-p1

Basic Information

• Species: Cyphellophora europaea
• Lineage: Ascomycota; Eurotiomycetes; ; Cyphellophoraceae; Cyphellophora; Cyphellophora europaea
• CAZyme ID: HMPREF1541_01691-t46_1-p1
• CAZy Family: AA3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1483	KB822718|CGC28	166197.60	5.0684

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CeuropaeaCBS101466	11153	1220924	59	11094

• Gene Location: location=KB822718:565869-570371(-)

Full Sequence      

MRPQKVPWRGALILSLACLLESHAAWASHSVNVALQTSFNAAPFLVELLETAAAESPASY
FPLLDRIAQRTFAEAQTEKELYERFIQVLQDDGHFTDGESLASFKFALSLHEAAPRIEAH
FQYYNATVQPHMMAAQDAVCPVWVHFDGQQYCSPALERAQQPVAFETSENVLPFDRVLGP
AEAPASIIYADITHPLFADFHNTISETARDGTTSYRIRYRPSETRSEPVPLSGYGVELNL
KRTDYIVIDDREAGSADEVKQDAAKEPDANDELQIKSLTTSELYSLGLNAASWVASNPNP
MQALLQVSTDFPKYSSFLASQDANEDFLKEHLSNREILLPSGFNALWINGLQIENRQVNA
YSLLEHLRRERKLIGQMKDIGLTPTEAVNILAHEVIAESARNAADHRFDWRDDLEGGEVI
IFMNDLEQDKRYTGWPTSLTGLFQRTYPGQLPAVARDIHNIIVPFEVDNLKDLELVTTAL
QSLIKRKVPIRIGLVPIGASKAAQTYGKLSYHLLDTYGLATFLSFYQETVKSGKLAAQPE
KLFAATIKERKLRQDKTALSYTEVLESEELEQRLRHRDAYVDRLELRGSTAPFIVNGVVL
ARTESWFETMSQRVYMDLQVIMQAVYEGSLPEDASARDHLIAEAITRRNELVIPHDLKDI
KVVDVAALTADHADAFAKMPRIVGAEAVLMADRAHMLVVADLNTEDGRSLASAAVEFQTK
VPEIEVMLVHSAGDPSEPGISTLMYHWQRETGPKLSAEQQAKVQAFLGGSDAKIRMPQEV
QQEAAQYWALANGLIRDLGLKAGENGLWLNGRMLGPLRRSLGVEDLQLLLDFERSARLAP
VTMAVAATGLEDKFKEPLDVAKITAVVARSQKSELPEGMRDSAPLLRLDRYRLWNDTHTA
ITVGTSDDPTLQVVAVLDPVSEVSQPWISILHTLSELEGVNLKIFLNPRGQLTELPSKRF
YRQVLGSAPSFDADGIRVSPKASFNKLPEDTLFNLDLILPPSWLVAPKTSVDDLDNIKFS
GTEHDVHAVYELEHILIEGHARDVTVNPPPRGVQLLLGTAEDPHFTDTIIMANLGYFQFK
ANPGYWQISLKPGRSSKIYNLDSVGPDGYLAVPGDETSSVALMSFQGTTLFPRLSRKPGM
EEAEVLESTTPGSAMDYLNKGASFASSALSSLGIKTSSTTNADINIFSVASGHLYERMLN
IMMVSVMKHTKHSVKFWFIEQFLSPAFKSTLPSLAEHYGFEYEMVTYKWPHWLRAQTEKQ
REIWGYKILFLDVLFPVDLDKVIFVDADQIVRTDMMELNKVNLKGAPYGFTPMCDSRTEM
EGFRFWKQGYWETYLRGKPYHISALYVVDLKKFREVAAGDRLRQQYHSLSADPASLSNLD
QDLPNHMQHNLPIFSLPQEWLWCETWCSDESLKDAKTIDLCNNPQTKEPKLDRARRQVPE
WTEYDNEIREVIAKARGDVPAMQQAGDSVAQADAQTPLKKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1184	1431	1.5e-134	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1184	1431	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1184	1451	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	1.78e-77	408	652	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	2.85e-65	1038	1147	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
132996	Glyco_transf_8	1.45e-60	1184	1431	1	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UDD64319.1|GT24	0.0	8	1473	11	1472
BAE65008.1|GT24|2.4.1.-	0.0	8	1473	11	1472
QRD91624.1|GT24	0.0	8	1473	11	1472
QMW47293.1|GT24	0.0	8	1473	11	1472
QMW35231.1|GT24	0.0	8	1473	11	1472

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	21	1460	1	1456	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	21	1460	1	1456	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	30	1148	5	1125	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	21	1460	1	1456	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	21	1460	1	1222	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	3.60e-276	26	1455	17	1428	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	4.80e-225	10	1483	26	1555	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q6P5E4|UGGG1_MOUSE	4.58e-224	12	1451	29	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9JLA3|UGGG1_RAT	4.58e-224	10	1451	26	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	5.96e-224	28	1454	27	1510	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000694	0.999293	CS pos: 27-28. Pr: 0.9190

TMHMM  Annotations     

There is no transmembrane helices in HMPREF1541_01691-t46_1-p1.