CAZyme Information: HMPREF1541_00965-t46_1-p1

Basic Information

• Species: Cyphellophora europaea
• Lineage: Ascomycota; Eurotiomycetes; ; Cyphellophoraceae; Cyphellophora; Cyphellophora europaea
• CAZyme ID: HMPREF1541_00965-t46_1-p1
• CAZy Family: AA3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1075	KB822711|CGC8	116409.87	4.1037

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CeuropaeaCBS101466	11153	1220924	59	11094

• Gene Location: location=KB822711:2534461-2537788(-)

Full Sequence      

MQSPCGALLLLMLITSVTALDSSGSFSLPNKLTRRATADADVYETRFDGVSWDNANWQLT
TETLDQGHYQARLSVANGYHGINVASLGPFFELDTSVDGDVIGGWPLFDRRQTFATIAGF
WDSQPTTNGTNFDWLEQYGWESAISGIPHWAGIIVDLGQSAFLDASTNSSEIDDFTSTLD
MKNGVVSWSFVWSPPDHDSFNISYQMFAHKLHVNQAFIQMNITASSDANATVANVLNGDC
AVRTQPGEKGTDGGMIFSSVHPDGLENVTAFIYATMAVDGADPSNTSEGDLDRPYVGGNE
SSIATGLDIALSASQTVTFTKFVGIASSDGFEDPKSIARDAAQSANSTGYLPSLASHASE
WAIVLPEHSVDDFADPLNGSLPDDPYAVESAILAVANPYYILQNTLSENAMSAVSNASIN
SHSVSVGGLGSDAYAGQIFWDAETWMQPGIVATFPYAARGFSNYRLEHYQQALENIDTAY
QSSKNETEFSSDAAIFPWTSARRGNCTATGPCWDYEYHINGDIAQALSNEWLVSGDTDFF
EQNHFPIHDSISVLLSEILEKNGSQWALRNMTDPDEFANHVDNGAFTMPLIANTLTNNNF
FRTLFNQTPNDTWAEQAENVVIGRDESANILLEYTGMNGSIEVKQADVVLVTYPLSYTGE
NYTANDSLSDLDYYAAKQSQDGPGMTYAIFSIVANEVSPSGCSAYTYAVYSHHPYVRAPW
FQFSEQLVDEFSVTGYHPAFPFLTGHGGANQVVLFGYLGLRLLPTSWSLHINPALPPQVP
QVRYRTFYWHGWPISAFSNQTHTTLTRDPALEGAPGTSPNSTYATASIPVIVGAPGAEDT
TSYDLPPNGTLVVPNRQYSLLKTTPGNALQCQFAESEADFVPGQFPIAAVDGAASTKWQP
TESSENATITVEIPEVDQGKEVTGIRFDWGQRPAWNYSVSFANSSDEDGQVVAAGEADIS
DPWDEQEIARIVAVQSNTTYVNLTGTAQGGGGDEGPVVAPRYATLTIWGHRFDTNVTAEN
MTGDGATVAEWEVIVADDGGGADAPVRREMGVFGRQMLERAASGEKRGRKGKARL

Enzyme Prediction     

EC	3.2.1.28:5	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	413	762	6e-73	0.9489247311827957

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	6.74e-61	56	788	10	724	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	9.51e-39	60	328	1	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
281612	Glyco_hydro_65m	3.00e-17	423	697	19	332	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.
237517	PRK13807	1.07e-10	57	362	10	300	maltose phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UDD58012.1|GH65	0.0	12	1060	11	1071
QMW44967.1|GH65	0.0	12	1060	7	1067
QRD84477.1|GH65	0.0	12	1060	7	1067
QMW32935.1|GH65	0.0	12	1060	7	1067
BAE62687.1|GH65	0.0	12	1060	11	1067

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	3.30e-33	161	776	89	656	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	7.26e-15	409	791	310	722	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4B5H9|ATC1_ARTBC	0.0	43	1034	53	1051	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|P78617|TREA_EMENI	0.0	41	1034	48	1037	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|M1RNJ8|ATH1_CANGB	3.51e-115	33	953	143	1069	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|Q5AAU5|ATC1_CANAL	1.17e-114	60	940	112	965	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|N1P212|ATH1_YEASC	5.53e-106	47	946	121	1041	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000284	0.999704	CS pos: 19-20. Pr: 0.9796

TMHMM  Annotations     

There is no transmembrane helices in HMPREF1541_00965-t46_1-p1.