CAZyme Information: HCEG_02405-t36_1-p1

Basic Information

• Species: Histoplasma capsulatum
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum
• CAZyme ID: HCEG_02405-t36_1-p1
• CAZy Family: CE5
• CAZyme Description: proteasome activator subunit 4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2188	DS990637|CGC6	247617.28	7.1451

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HcapsulatumH88	9666	544711	221	9445

• Gene Location: location=DS990637:2504561-2512003(-)

Full Sequence      

MRLTASSPSPRWQWICGTLLDHPLSSLSVLFVFWKLLLLLAVVASPGPGYDTSTTLLPST
ASASATDSSLLGVGTVIGGGSGWSSSLLKLVRWDAIYFVSIAQRGYIFEQEWAFGYGYTK
LLSFLSSGESFLVFWGVPDSNPDTLHVALTGIGLSHTCHYLSVLSLYGLSRTVFGDGSSR
ALPLLAAALHVISPAGAFLSAPYGEPVFSFLNFTGFYAYASALHDDRRGCVFARDVKFLV
AGGLFATVTTVRSNGVLSGMLFAYDTVVGLAAIVSSGVSLSKLHRMFFVVLGGSLILLGV
VGPQYRAFMVYCRNVDVRRIWCDNTIPSVYTFVQNHYWGSGTFLSYWKVSNLPLFCLAAP
MLVIMGVSSWWPFQSPLDFDIRNKEPRKREEEVSQTEHRRTCLYRLAIPQAVLTVLALTN
SHVQIINRISSGYPLWYWYIAHSLQGAALLVSGPANNLIRSSLFQKQVRTFQLSFGQLVA
PKSNELGYLLHQRQTGLYSIPSTSSRPSSPFAIFTFNANMDSREQLPDILVADHGHSENE
LSRETSPGGEPTRINGGLLDDHFKKARNRPRTYPYFKYLPYTVEDESERQRSLEEILEHL
YIAVESGDFNPGAVHWTRELKGWLSLKFDPTREQRIKLVKFYYELALAPGVDPNVAERFA
SMFMLLTKRKHYLRPVKDLILDWKPLYREIKIFVLPSQSGLVHSTNLKRNIKTLTKLCAF
AQPYFDPLELPVMLEEFLPHFSTSFIEGAFVVIGLMNLFLPTTPPLEDREDLLPQHYLPT
YFHLWPLVNRSRTFDVTFTDLLSRMARDALPASYIPFSEFGIFTSEQTTLIFTAILRLME
IPVGQATSPYSSGVDSLSGLGLMLERDFRKHPFTHNIARWIVMSLSPACLETEHSILSKL
EGLIEAVETFFHPSNTGVWSKALSQLVYYLADFFVMRWNMEHDKENEVPPERRLNDALKR
RFVLCLREVTFMGIYSKLGVAMNFALSTLQSLAYLEPKLILPGALQRIYPSLQGLVEVHR
TTSSLRSLQVLSRLMARTKGFRCHITTLLGLVLPGIDANDLEKSLHSLAFIQSVCYNIPF
EDLTRDRDDVNGNMLAVQWITGELERMENEGASIEMNYDTELTDQEEEMILRSSTTQFGE
FLLSFLGRVFTLLENLPDAARVRSGSPEENVVNTLPATLMPLLASLSPELYDQALIKIVN
FVSGHVIHPARDAMAFICNAVCKVNPEKALKSLVPLLIQSIRTEIDENGAASTRTTGSDI
LPRDRGLVWNVSMLSMCVVHVGSAVLKYRTDLFDIAIYMQQKCKGIPTVHVSNFIHHLLL
NLTATYVIDHSLYEPDVKGKGIQVEQWGQRHDPNHLTIKWHVPQREELEFAVELFKSQAE
NALDQLTSLTSETPSINRDGSGKEWSDEVTRKLVLLRLIISGISMLFDNKAASYYNTGDR
RSHADLDVKMSNANDPCSDEGSSEDDSALDGSDDSAVRQTFQYPTGYLLKEDDPLYISIH
EIRRRVGEVLHNVHRFLVEKQEDDVSCFPPLYTAYRSWFVDVGIEKSAHILDRVTKLLAA
DTQPYKVSGVRKDYPRPLLVRRANVYHVQRLRHNAFPRPRSKLDETLLLDLAESSVSLYT
DIRRSAQGASESALKAIWGSRLLIIPTLIKALQTAIKTNDYPRLKGAIYSILFSSIAKPV
GRHWKYAPSLIKAFIEANAVDRPSIQKLCSGAVYQIMDYGRPMERMAVLDQDIIKSIAPK
SDVQAIIDNKREALQKKRALIEQKKAELSEELTDLARVSHWKMASRTAAIVIGLGLRFDY
VASENLVNLIIQGSIDSHPSLRGIYSQTLVALFTMVDVRAVCSHKYENYIMGVQRFPSKI
QVATQPYKKGWTEEYLASFARPEAEYYVDHDYPGWLVWGKTMPAYKSNIKQDIEYDELEW
NVRKQMGKLCTREWFAAFFKYLKQEPRDVYADKFRMSSAMTLLYVFELMLRDELTAATFE
DIKEEIAVVFEDGSDKHQHRATAEILGALIGSVTDTSVDKRTLVWEYAFPIVCRIFSDGL
TPENSGYWTTFVHMIMQCRDPRRLWPLLDWLTSFRLDMNSNAAFKESSKINLLHQCILDV
GWHFQLEKPIVADFLAHLDHPYKGVREAIGHTLGSIYRTRYHESYENIQQLIQAQNEASS
IGTRPYKPSKEFADTISDVFSRLERMAR

Enzyme Prediction     

No EC number prediction in HCEG_02405-t36_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT76	30	448	6.5e-93	0.914004914004914

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
406818	BLM10_mid	0.0	900	1427	1	499	Proteasome-substrate-size regulator, mid region. The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_mid is the middle ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. Thus Blm10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.
282094	Mannosyl_trans2	1.78e-32	90	445	57	396	Mannosyltransferase (PIG-V). This is a family of eukaryotic ER membrane proteins that are involved in the synthesis of glycosylphosphatidylinositol (GPI), a glycolipid that anchors many proteins to the eukaryotic cell surface. Proteins in this family are involved in transferring the second mannose in the biosynthetic pathway of GPI.
406851	BLM10_N	3.10e-30	537	612	1	81	Proteasome-substrate-size regulator, N-terminal. The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_N is the N-terminal ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. BLM10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.
227829	COG5542	2.07e-12	92	444	76	367	Mannosyltransferase related to Gpi18 [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS49377.1|GT76	1.13e-294	1	441	1	436
QSS60603.1|GT76	1.20e-236	67	441	47	420
BCR82784.1|GT76	7.38e-112	22	440	19	419
QGA17664.1|GT76	1.61e-110	20	447	19	431
CAP97902.1|GT76	6.32e-110	20	443	20	410

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6KWX_A	1.43e-71	575	2137	69	1548	cryo-EM structure of human PA200 [Homo sapiens],6KWY_c human PA200-20S complex [Homo sapiens]
6REY_c	2.37e-71	575	2137	34	1513	Human 20S-PA200 Proteasome Complex [Homo sapiens],6REY_d Human 20S-PA200 Proteasome Complex [Homo sapiens]
4V7O_A5	2.96e-39	615	1428	4	793	Chain A5, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_A7 Chain A7, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B4 Chain B4, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B7 Chain B7, Proteasome activator BLM10 [Saccharomyces cerevisiae]
4V7O_A6	5.60e-37	1490	2174	17	700	Chain A6, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_A8 Chain A8, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B5 Chain B5, Proteasome activator BLM10 [Saccharomyces cerevisiae],4V7O_B8 Chain B8, Proteasome activator BLM10 [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2UJS7|GPI18_ASPOR	1.53e-103	21	479	16	417	GPI mannosyltransferase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=gpi18 PE=3 SV=1
sp|Q4WQ21|GPI18_ASPFU	1.07e-95	2	444	3	399	GPI mannosyltransferase 2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=gpi18 PE=3 SV=1
sp|F1R2X6|PSM4B_DANRE	1.28e-75	575	2137	19	1495	Proteasome activator complex subunit 4B OS=Danio rerio OX=7955 GN=psme4b PE=3 SV=2
sp|Q5SSW2|PSME4_MOUSE	1.53e-74	544	2137	5	1513	Proteasome activator complex subunit 4 OS=Mus musculus OX=10090 GN=Psme4 PE=1 SV=1
sp|P43583|BLM10_YEAST	2.04e-74	615	2174	242	1846	Proteasome activator BLM10 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BLM10 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.991758	0.008259

TMHMM  Annotations     

Start	End
27	49
69	91
112	134
147	169
181	203
207	224
255	277
287	309
351	373