CAZyme Information: HCDG_06863-t38_1-p1

Basic Information

• Species: Histoplasma capsulatum
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum
• CAZyme ID: HCDG_06863-t38_1-p1
• CAZy Family: GT2
• CAZyme Description: alpha-1,3-mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
358		40356.79	9.9446

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HcapsulatumH143	9796	544712	249	9547

• Gene Location: location=GG692430:680832-682144(+)

Full Sequence      

MNIYERAIGLATTPEYSRWICPLLLLAEVFLCALIVWKIPYTEIDWSTYMQQVSLFLSGE
RDYALIKGSTGPLVYPAVHVYIYSVLYRICDEGRDIAFAQVVFAILYIATLVIVMWSYRS
ARAPPYLFPLLVLSKRLHSVFLLRLFNDAVATFFLWAAILMLQRRQWTAGVVLWSMGVGV
KMTLLLIAPAVAVIVVLGAGIIKATGLGITALLLQVLLAVPFLQENPSSYMLRAFEFTRQ
FLFKWTVNWRFVGEDTFTSQSFALALLAVHASILALFFVYRWIGPSNASFTQFIRKAVHG
KLPPTQGYSAPAPCTINFSRIFHGQRLFFSGSLDSTRCLFICFGPRRNGLGTSIQAQR

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	22	298	2.9e-116	0.7582417582417582

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	1.58e-147	22	312	1	291	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS55330.1|GT58	2.23e-211	1	306	1	306
QSS72893.1|GT58	1.74e-208	1	306	1	306
QSS65569.1|GT58	7.70e-169	50	310	1	261
QVM12997.1|GT58	4.14e-137	1	301	1	301
BAE62911.1|GT58	1.94e-127	10	301	10	301

PDB Hits     

HCDG_06863-t38_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2U6A4|ALG3_ASPOR	3.44e-128	10	301	10	301	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	9.26e-117	1	298	9	306	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	3.17e-114	10	296	72	358	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1
sp|A1DDZ3|ALG3_NEOFI	9.82e-112	1	298	1	298	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=alg3 PE=3 SV=1
sp|Q9C1K8|ALG3_NEUCR	5.60e-105	3	304	14	321	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000026	0.000002

TMHMM  Annotations     

Start	End
19	41
96	118
140	162
172	194
201	223
262	284