CAZyme Information: HCDG_06000-t38_1-p1

Basic Information

• Species: Histoplasma capsulatum
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum
• CAZyme ID: HCDG_06000-t38_1-p1
• CAZy Family: GH76
• CAZyme Description: glucoamylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1360		150176.27	6.5690

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HcapsulatumH143	9796	544712	249	9547

• Gene Location: location=GG692428:657138-662318(+)

Full Sequence      

MAVSLFCGALLFVGLWSVFAGLWAGLVDLQPWPIQRPLLTNPLEAWIAKESSYALQALLA
NIGAEGAKVKGAVAGIVVASPSKEDPDYFYTWTRDASLVFKGLVDAFISGDTSLQSKIHE
FISAQAHLQTISNPSGGLSSGGLGEPKFDVNMSSFNGNWGRPQRDGPALRATAMISYGKW
LIANGYANTARMIVWPVVQNDLSYVAQFWSSPGFDLWEEVDGMSFFTTAVQHRALVEGSR
FARQIGLRCPSCESQAPQVLCFMQNYWTGSYINSNTGGDRSGKDTNSILASIHTFDPEAS
CDDLTFQPCSAKSLANHKVVTDSFRSIYPLNHGVSAGSAVAVGRYPEDVYFGGNPWYLST
FAAAEQLYDAIYQWQRLGSILVTDVSIHFFKDIYASAVVGTYPSSSTTFKSIIEAVRDYA
DGYLAVAQKYTPVSGELAEQFSRDDGTPLSASDLTWSYASFLTAVSRRNSSVPDPWGQTA
ASSIPSTCQITSATGPYRTATNTKWPSDLTSISRTTSFSNGLTETKITTTSTTTSTSPCV
TPDSIRVTFNEVATTTPGQRIFIIGSVPELGSWDVLSAIALSADQYTDDNRLWHRTIQLG
AGLDFEYKYIRLSMRTEANAIMQKPACLRISCFCEKHSSQMPHRMSWSAVSTPFQKWEIL
CHKWMMLPVPMSWAKHSVTAFSVPKCRPLRGCLEHETEVSPPTRRLLILALQDDKARFIH
FSRIAWRLVDIVSVNYLQTTMSFSDSLPVSDTSQELFGSAEWLLSPPAFVAQKGDHGPAQ
VTFPPPGAESETGFRTRQRVSLACLPCRNRHVKCDSGLPICSRCKFDGRQCKYMQSRRGG
HNKIVSSDTRAPVTQSPTRGSEFSHRSSGDRTSTRVGDSDSAGMVETGTSSESAATTGSA
SLDRPFECYYTFFHESHPMVLPKQHFTARLDVDPESLEVVAAIMRYIGSLYGHCPSPEFF
KSLSESLLFCNQLPRNGFSVQALMLYSIATHWNNDKPRAREILDVATTMALEIGMNSCQF
AYQHGEGNLVLEETWRRTWWLLYIVDSLYAGIRHSPGFALWSVNSNVDLPCEEEDFHSGN
IPKPKTLVEFDDRQFDTVDVVFSSFAYLIDASRILGTTLAVGLDDSNPVDPEVDYVDLGL
VAWSLHLPDIKRELVTNKGNVDPILFLAHMLIHTTTIYLHRPRSKLLYNNAENISKCAPP
PPPERLTHAAQEAYQMHTVKVLTASEKISSLLALPTPLVKQSQFIICMVALSTIAQLSAC
IFDTNIGTSRAIKERIRLNIGALKAHEKIWPLGRKTLYEVKLIARQVFALPNLDCQVISD
LMDQAHEISNHSGNSVMIDESFPTAIFGDSCFLSPVPSPL

Enzyme Prediction     

EC	3.2.1.3:94	1.14.99.55:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH15	58	465	9.8e-78	0.9861495844875346
CBM20	545	614	6.8e-17	0.7222222222222222

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395586	Glyco_hydro_15	5.37e-134	54	464	3	415	Glycosyl hydrolases family 15. In higher organisms this family is represented by phosphorylase kinase subunits.
99886	CBM20_glucoamylase	8.67e-35	539	611	1	73	Glucoamylase (glucan1,4-alpha-glucosidase), C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Glucoamylases are inverting, exo-acting starch hydrolases that hydrolyze starch and related polysaccharides by releasing the nonreducing end glucose. They are mainly active on alpha-1,4-glycosidic bonds but also have some activity towards 1,6-glycosidic bonds occurring in natural oligosaccharides. The ability of glucoamylases to cleave 1-6-glycosidic binds is called "debranching activity" and is of importance in industrial applications, where complete degradation of starch to glucose is needed. Most glucoamylases are multidomain proteins containing an N-terminal catalytic domain, a C-terminal CBM20 domain, and a highly O-glycosylated linker region that connects the two. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
213391	fungal_TF_MHR	1.45e-32	906	1300	10	401	fungal transcription factor regulatory middle homology region. This domain is present in the large family of fungal zinc cluster transcription factors that contain an N-terminal GAL4-like C6 zinc binuclear cluster DNA-binding domain. Examples of members of this large fungal group are the following Saccharomyces cerevisiae transcription factors, GAL4, STB5, DAL81, CAT8, RDR1, HAL9, PUT3, PPR1, ASG1, RSF2, PIP2, as well as the C-terminal domain of the Cep3, a subunit of the yeast centromere-binding factor 3. It has been suggested that this region plays a regulatory role.
395557	CBM_20	5.81e-24	545	613	1	68	Starch binding domain.
99883	CBM20_alpha_amylase	1.71e-18	547	611	3	63	Alpha-amylase, C-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in several bacterial and fungal alpha-amylases including the maltopentaose-forming amylases (G5-amylases). Most alpha-amylases have, in addition to the C-terminal CBM20 domain, an N-terminal catalytic domain belonging to glycosyl hydrolase family 13, which hydrolyzes internal alpha-1,4-glucosidic bonds in starch and related saccharides, yielding maltotriose and maltose. Two types of soluble substrates are used by alpha-amylases including long substrates (e.g. amylose) and short substrates (e.g. maltodextrins or maltooligosaccharides). The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS49369.1|CBM20|GH15	0.0	1	611	1	611
QSS67842.1|CBM20|GH15	0.0	1	611	1	611
QSS60609.1|CBM20|GH15	0.0	1	611	44	582
CAC28076.1|CBM20|GH15|3.2.1.3	3.94e-252	40	611	29	583
ABH92242.1|CBM20|GH15|3.2.1.3	3.94e-252	40	611	29	583

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FRV_A	1.61e-225	43	612	3	582	Structure of the catalytic domain of Aspergillus niger Glucoamylase [Aspergillus niger]
1AGM_A	1.58e-211	43	507	3	467	Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. x100 to 2.4 angstroms resolution [Aspergillus awamori],1DOG_A REFINED STRUCTURE FOR THE COMPLEX OF 1-DEOXYNOJIRIMYCIN WITH GLUCOAMYLASE FROM (ASPERGILLUS AWAMORI) VAR. X100 TO 2.4 ANGSTROMS RESOLUTION [Aspergillus awamori],1GLM_A REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100 [Aspergillus awamori],3GLY_A REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100 [Aspergillus awamori]
1GAH_A	1.63e-211	43	507	3	467	GLUCOAMYLASE-471 COMPLEXED WITH ACARBOSE [Aspergillus awamori]
1GAI_A	1.69e-211	43	507	3	467	GLUCOAMYLASE-471 COMPLEXED WITH D-GLUCO-DIHYDROACARBOSE [Aspergillus awamori]
3EQA_A	2.70e-209	43	507	3	468	Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol [Aspergillus niger]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P36914|AMYG_ASPOR	1.06e-240	43	612	30	579	Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2
sp|P22832|AMYG_ASPUS	1.11e-229	43	612	27	605	Glucoamylase OS=Aspergillus usamii OX=186680 GN=glaA PE=3 SV=1
sp|P0DN29|AMYG_ARTBC	6.97e-228	55	611	39	576	Glucoamylase ARB_02327-1 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02327-1 PE=1 SV=1
sp|P23176|AMYG_ASPKA	1.07e-226	43	612	27	605	Glucoamylase I OS=Aspergillus kawachii OX=1069201 GN=gaI PE=1 SV=1
sp|P69328|AMYG_ASPNG	1.92e-224	43	612	27	606	Glucoamylase OS=Aspergillus niger OX=5061 GN=GLAA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.971991	0.028031

TMHMM  Annotations     

Start	End
5	27