CAZyme Information: HCBG_08398-t26_1-p1

Basic Information

• Species: Histoplasma capsulatum
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum
• CAZyme ID: HCBG_08398-t26_1-p1
• CAZy Family: GT39
• CAZyme Description: glycosyl hydrolase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1094	GG663377|CGC2	119002.12	5.2380

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HcapsulatumG186AR	9364	447093	110	9254

• Gene Location: location=GG663377:491993-495513(+)

Full Sequence      

MLCKFRSWSFVLLLLSSSAISALSFTERVATVFAKNYISSDSSTKCDGADCQEFLYPTRF
PGVTWDHDNWRLATTTLYQGHYQSRGSIANGYIGINVAAVGPFFELDTAVNGDVINGWPL
YSRRQTFATISGFYDLQPTTNGSNFPWMNQYGGESVISGVPHWSGLVLDLGNNKYLDSSV
TMDEISNFKSTLDMKGGLLNWEYTWSPTGRGESFAISYQLFAHRLNVTQAVVRLAITPSA
DMNVKVVNVIDGYSAVRTEFVDSGEDGHAIYSSVSPVGVPDVTAYIYATMSGSKEVDIES
LKLVPNPPYVHTNRSSIAQAADIKLRAGQKSIVTKFVGVASSDGFENPKQVAKEASSDAA
DIGYDALLKSHIGEWATVFTDDSVDDFAVPKTGRLPLDDHVIESAITSVTNPFYLLQTIV
SDNAIHAARNAPIHQGSVAVGGLTSDSYGGLIFWDADIWMQPGLVTAFPQAAQSFTNYRL
IKYGQAIENTKTAFASSKNQTRFSQGAAIYPWTSGRFGNCTGTGPCFDYQYHLNGDIGLQ
MINNWVTTGDTDHFKEKLFPVYNSIAILYADLLEKNGTKWTLTNMTDPDEYANHVDGGGF
TLPMMATTLIYANSLRQMLGFEQNETWNEMAENILVLRDPVADVTLEYTTMNGSTLVKQA
DIVLNTFPLRYTDGYSPQNALSDLDYYAAKQSPDGPGMTYAIFSIVANEISPSGCSAYTY
AQYSYAPYIRAPFFQFSEQLIDNWSRNGGTHPAYPFLTGNGGANQVALFGYLGLRLLPDF
VLHLDPNLPPQIPHLSYRTFYWHGWPLKAASNYTHTTIQRAPNTQPLPTADKKFAGAPIP
VHVGPESNVTVYLLPASGILTIPNRQIGSHATFPGNMVQCQPASSPDEYEPGQFPIAAVD
GAASTKWQPKRANATSSLTVSFPDDEISELITGFGFDWAQAPPESIAVVLHNTPISKPTQ
QQVSQSFSPSSASGALSVPSTPGAITVTAFPSITLSNPYNPATTDLNTIARYAGNTTNVT
LARPVPATRFATLFMWGNQALDNADRRAGNGSGATVAEWSILRALRAGQSGTTAGRGGGE
EGKFKVRGARWWGE

Enzyme Prediction     

EC	3.2.1.28:5	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	412	776	4.6e-77	0.9811827956989247

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	2.94e-69	68	801	9	724	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	5.65e-43	78	342	9	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
281612	Glyco_hydro_65m	1.62e-21	411	776	3	382	Glycosyl hydrolase family 65 central catalytic domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. The central domain is the catalytic domain, which binds a phosphate ion that is proximal the the highly conserved Glu. The arrangement of the phosphate and the glutamate is thought to cause nucleophilic attack on the anomeric carbon atom. The catalytic domain also forms the majority of the dimerization interface.
237517	PRK13807	1.44e-17	66	634	6	529	maltose phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS73854.1|CBM32|GH65	0.0	1	1094	1	1094
QSS66944.1|GH65	0.0	1	1088	1	1088
UKZ51236.1|GH65	0.0	48	1062	43	1041
UKZ77068.1|GH65	0.0	48	1062	43	1041
QYS98630.1|CBM32|GH65	0.0	27	1062	28	1042

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	6.49e-37	68	776	22	642	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	4.81e-16	317	804	226	722	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]
4KTP_A	4.72e-11	423	849	301	751	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTP_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]
4KTR_A	8.15e-11	311	849	215	751	Chain A, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_B Chain B, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_C Chain C, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_D Chain D, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_E Chain E, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_F Chain F, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_G Chain G, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10],4KTR_H Chain H, Glycoside hydrolase family 65 central catalytic [[Bacillus] selenitireducens MLS10]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P78617|TREA_EMENI	0.0	56	1062	50	1037	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|D4B5H9|ATC1_ARTBC	0.0	18	1063	20	1052	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|M1RNJ8|ATH1_CANGB	2.35e-120	60	948	157	1055	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|Q5AAU5|ATC1_CANAL	2.64e-120	67	948	106	964	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|P48016|ATH1_YEAST	1.58e-111	60	948	121	1034	Periplasmic acid trehalase ATH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ATH1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000536	0.999418	CS pos: 22-23. Pr: 0.9639

TMHMM  Annotations     

There is no transmembrane helices in HCBG_08398-t26_1-p1.