CAZyme Information: HCBG_02565-t26_1-p1

Basic Information

• Species: Histoplasma capsulatum
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum
• CAZyme ID: HCBG_02565-t26_1-p1
• CAZy Family: GH13
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1507	GG663365|CGC1	168659.84	5.1371

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HcapsulatumG186AR	9364	447093	110	9254

• Gene Location: location=GG663365:1201822-1206637(-)

Full Sequence      

MIFRYYTFPWRLGGLVLFGAASLFTSHATAGPSVNVALQTSFNAAPYLVELLETAADENS
TSYFPLLDRISEGTFTEITTEKGLYERFIQLLQEDGHLTNPESISSFKFALSLRSTAPRI
EAHYQYYNTSVEPSLMIAQDAVCTVWAHYDGDQYCSPSLEYAQQSVSWDGHDRVLPFDRV
FGDPSLPPLILYADVESPLFGGFHHQLMQQARDGQFSYRVRYRPASSATSRPLFVNGYGV
ELALKRTDYIVIDDRDAEQRVLKDTDTTNPTLAPAEDLENEQPADLKPLSASEVSTLGMN
AASFIMSSDDPFATLLRLSQDFPRHSSAIAGASRTSEFTQEFEQNKDNHLQTGRNVIWVN
GLQMEAQTVDAFSLLDHLRRERKLINDLRKFGLSARQAVDLLTNPTISKSLGTDDSIRYD
YRDDLEGGGVIVWLNDLEKDHRYEGWPRDLHSLFIMTAHGQLPPVRRDIHNVVFPVDLAS
SEDVSVVSQSLLKAIKARIPVRFGIVPLVHNENAQGQAKIVHHLLDTYGLGAVMNYLQAS
LSADKIGSPDQASFTAALGNHELRKDRTPLTFEDVLRSDNYDPIVSNTKSYLKRLAVKGD
ILPFFVNGATLMREGNFLQNMITALSKDLEHLQRRVYEGAFEEDIWIPSHFLQEALQNRS
PLLIPENPSEIQITDLNEIYANHPNVFDTILRIPASVESDDPMLDWNSIILIADLDSGAG
AELLSSLLELHKKHPGVEILLLHNGESPSTPEALSTRLYSIRNGRDLDPAVVTAALASKN
DDSASDSAAASAYWNTVQFLVKEIGFGTGEIGMVVNSRILGPLPSSTVLGTEDLENLLMY
EHSKRIGVLARVASEMDLDSNILDPLSLARMQAVLSLSATSGISESIYNYAPSARTRVFE
KWSGIHTAISVSNSDDPSINIVATIDPTTEEAQRWVPILKVLSELGGVSLKLFLNPREEI
KELPIKRFYRHVLDVAPSFNEDGSLAKPQAVFHGIPGEALLNLGMDVPPAWLVAPKESIH
DLDNLKLSTLRAGTNVDAIYELEHILIEGHSRDITQGGPPRGVQLLLGTEKSPHFADTII
MANLGYFQFKAQPGCWKITLKPGQSERIFHIDSVGGLGSKPTPGDESNDVALLSFQGKTL
FPRISRKPGYENEDVLDEVNKPATAAKDFLAKGLNFASEILHRLAGPAQGTHADINIFSV
ASGHLYERMLNIMMVSVMKHTKHSVKFWFIEQFLSPSFKSFLPHLAAEYGFSYEMVTYKW
PHWLRAQTEKQRIIWGYKILFLDVLFPLSLDKVIFVDADQIVRTDMYELVTLDLEGAPYG
FTPMCDSRTSMEGFRFWKQGYWKNFLRGLPYHISALYVVDLKRFRAIAAGDRLRGQYHTL
SADPQSLSNLDQDLPNNMQRMLPIKSLPQDWLWCETWCSDESLATAKTIDLCNNPLTKEP
KLDRARRQVHEWTVYDEEIAAVQRRVLEEEEKERMGAVIEGGDEEVPRNEGGDHGDGKNK
KHKKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1195	1442	4.1e-134	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
133054	GT8_HUGT1_C_like	0.0	1195	1442	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408203	Glyco_transf_24	0.0	1195	1462	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
408201	Thioredoxin_14	8.61e-88	419	663	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	2.55e-61	1048	1157	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.
408199	Thioredoxin_12	9.59e-61	44	231	1	185	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS69256.1|GT24	0.0	1	1507	1	1507
QSS51253.1|GT24	0.0	1	1507	1	1506
QSS63494.1|GT24	0.0	5	1507	16	1518
CAK39488.1|GT24	0.0	10	1493	11	1491
CAS97678.1|GT24	0.0	10	1493	11	1491

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	25	1472	2	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	25	1472	2	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	33	1162	5	1129	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	25	1472	2	1457	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	25	1472	2	1223	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	5.01e-284	18	1473	7	1435	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q9NYU2|UGGG1_HUMAN	2.68e-241	10	1471	23	1532	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	3.60e-240	10	1463	23	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	3.82e-239	15	1463	28	1524	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q09332|UGGG_DROME	1.14e-229	34	1470	30	1515	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.032241	0.967719	CS pos: 30-31. Pr: 0.8950

TMHMM  Annotations     

There is no transmembrane helices in HCBG_02565-t26_1-p1.