CAZyme Information: HCAG_01147-t26_1-p1

Basic Information

• Species: Histoplasma capsulatum
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Histoplasma; Histoplasma capsulatum
• CAZyme ID: HCAG_01147-t26_1-p1
• CAZy Family: AA7
• CAZyme Description: cysteine desulfurase, mitochondrial precursor

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
986		107298.80	6.5902

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_HcapsulatumNAm1	9402	339724	89	9313

• Gene Location: location=CH476655:4037711-4041696(+)

Full Sequence      

MALPSQERTTIGSFLTDEEIIEPTSDRYASATDFWTAYKNPKPQLVLRPKSLPALQKTVQ
YLCASDLDFSVRSGGNNTSGAKHVQLSMSAFDSFEFDQDSETVTIGAGQTWGEVDRKVEE
AAPGYTVLSTRVPFVGVAGSLLLGGMSWFSGEHGLCSDPHNLLDAHVVLPDGRMLWASTE
PDLLWALRGGGGNFGVVSAFKVKVHRCPQKIHAGTIAFPKTAMKAVSQGLSDFMKRCTDP
KLGAHVIVMNFDPPTDTDDVPQPGIAIIIFDSHGEEHGRSELGFKWAFDIEGAVDMTKVM
NIREVNEEGASLRSLAGNTIMLSSPLVDTSSIDPDFVVRIAEFYQQAVEANKALGSGTLV
VLEVLGKGALTSSGGSNMTAWPHTKPQHVLQLVVGHPPGADCSQELALGLLEKSPTQICP
SHKPGEYWPAFINESQNLNQIFGPNYERLRHIKTKYDPRASPQTGQLNNHAASLPSITMS
NLAPSAFRQALRAYPRRCASIRPAAPRTAAARRCYTSESKPARAQSSVDLDVDVAIKTGQ
RPFAEQTGIRPHNVTVPGTAVDSDAMMGPSTGILKQATVLDQGSRPIYLDMQATTPTDPR
VLDAMLPFLTGLYGNPHSRTHAYGWETEKATEQAREHVAKLIGADPKEIIFTSGATESNN
MSIKGVAKFFGRSGKKKHIITSQTEHKCVLDSCRHLQDEGFEVTYLPVQSNGLIDLKDLE
AEIRPETALVSIMTVNNEIGVIQPVKEIGALCRSKRVFFHTDAAQAVGKIPIDVNEWNVD
LLSISGHKIYGPKGIGACYVRRRPRVRIDPIITGGGQERGLRSGTLAPHLAVGIGEACRI
AKEEMEYDAKRIKALSKRLLDGLLSMEHTSLNGDPEKHYPGCVNVSFAYVEGESLLMALK
DIALSSGSACTSASLEPSYVLRALGNSDESAHSSIRFGIGRFTTESEIDYVLKAVRERVS
FLRELSPLWELVQEGVDLNTIEWSQH

Enzyme Prediction     

EC	1.1.3.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA7	31	238	6e-44	0.4585152838427948

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
178257	PLN02651	0.0	587	949	1	364	cysteine desulfurase
184450	PRK14012	0.0	586	986	4	404	IscS subfamily cysteine desulfurase.
131061	IscS	0.0	586	986	4	402	cysteine desulfurase IscS. This model represents IscS, one of several cysteine desulfurases from a larger protein family designated (misleadingly, in this case) class V aminotransferases. IscS is one of at least 6 enzymes characteristic of the IscSUA-hscAB-fsx system of iron-sulfur cluster assembly. Scoring almost as well as proteobacterial sequences included in the model are mitochondrial cysteine desulfurases, apparently from an analogous system in eukaryotes. The sulfur, taken from cysteine, may be used in other systems as well, such as tRNA base modification and biosynthesis of other cofactors. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other, Protein synthesis, tRNA and rRNA base modification]
224029	NifS	0.0	585	970	1	386	Cysteine sulfinate desulfinase/cysteine desulfurase or related enzyme [Amino acid transport and metabolism].
132443	FeS_nifS	4.66e-180	587	968	1	379	cysteine desulfurase NifS. Members of this protein family are NifS, one of several related families of cysteine desulfurase involved in iron-sulfur (FeS) cluster biosynthesis. NifS is part of the NIF system, usually associated with other nif genes involved in nitrogenase expression and nitrogen fixation. The protein family is given a fairly broad interpretation here. It includes a clade nearly always found in extended nitrogen fixation genomic regions, plus a second clade more closely related to the first than to IscS and also part of NifS-like/NifU-like systems. This model does not extend to a more distantly clade found in the epsilon proteobacteria such as Helicobacter pylori, also named NifS in the literature, built instead in TIGR03403.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UNI23960.1|AA7	1.28e-13	43	459	48	460
UPK90596.1|AA7	1.98e-13	43	470	20	446
QUC19098.1|AA7	9.10e-13	43	459	48	459
QRW27390.1|AA7	1.32e-12	20	221	43	248
VBB87167.1|AA7	3.09e-12	35	231	49	257

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5WGB_A	2.78e-207	570	986	11	426	Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and E. coli ACP1 protein at 2.75A [Homo sapiens]
6NZU_A	1.36e-206	585	986	3	403	Structure of the human frataxin-bound iron-sulfur cluster assembly complex [Homo sapiens],6NZU_E Structure of the human frataxin-bound iron-sulfur cluster assembly complex [Homo sapiens]
5WKP_A	1.52e-206	585	986	6	406	Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A [Homo sapiens],5WKP_E Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and Iron-Sulfur Cluster Scaffold Protein ISCU1, and E. coli ACP1 protein at 3.15A [Homo sapiens],5WLW_A Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A. [Homo sapiens],5WLW_E Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A. [Homo sapiens],6UXE_A Structure of the human mitochondrial desulfurase complex Nfs1-ISCU2(M140I)-ISD11 with E.coli ACP1 at 1.57 A resolution showing flexibility of N terminal end of ISCU2 [Homo sapiens],6W1D_A Structure of human mitochondrial complex Nfs1-ISCU2 (WT)-ISD11 with E.coli ACP1 at 1.8 A resolution (NIAU)2 [Homo sapiens],6WI2_A Structure of human mitochondrial complex Nfs1-ISCU2-ISD11 with E.coli ACP1 at 1.95 A resolution (NIAU)2. N-terminal mutation of ISCU2 (L35) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. [Homo sapiens],6WIH_A N-terminal mutation of ISCU2 (L35H36) traps Nfs1 Cys loop in the active site of ISCU2 without metal present. Structure of human mitochondrial complex Nfs1-ISCU2(L35H36)-ISD11 with E.coli ACP1 at 1.9 A resolution (NIAU)2. [Homo sapiens],7RTK_A Chain A, Cysteine desulfurase, mitochondrial [Homo sapiens]
5USR_A	2.22e-206	570	986	11	426	Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms [Homo sapiens],5USR_C Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms [Homo sapiens],5USR_E Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms [Homo sapiens],5USR_G Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms [Homo sapiens]
5KZ5_1	5.18e-200	595	986	1	391	Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_2 Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_3 Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_4 Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_M Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_N Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_O Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_P Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_Q Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_R Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_S Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens],5KZ5_T Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P0DN31|NFS1_ARTBC	4.15e-288	479	986	1	503	Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_05732-2 PE=3 SV=1
sp|P87185|NFS1_CANAL	2.50e-214	583	986	85	488	Cysteine desulfurase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=NFS1 PE=2 SV=1
sp|P25374|NFS1_YEAST	1.56e-212	531	986	54	497	Cysteine desulfurase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=NFS1 PE=1 SV=2
sp|P87187|NFS1_CANMA	7.83e-212	559	986	53	484	Cysteine desulfurase, mitochondrial OS=Candida maltosa OX=5479 GN=SPL1 PE=3 SV=1
sp|O60028|NFS1_ASHGO	1.40e-208	512	986	7	490	Cysteine desulfurase, mitochondrial OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=NFS1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000053	0.000001

TMHMM  Annotations     

There is no transmembrane helices in HCAG_01147-t26_1-p1.