CAZyme Information: H310_07910-t26_1-p1

Basic Information

• Species: Aphanomyces invadans
• Lineage: Oomycota; NA; ; Saprolegniaceae; Aphanomyces; Aphanomyces invadans
• CAZyme ID: H310_07910-t26_1-p1
• CAZy Family: GH89
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
768	KI913966|CGC1	84926.93	6.4977

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AinvadansNJM9701	15416	N/A	168	15248

• Gene Location: location=KI913966:1191469-1194113(-)

Full Sequence      

MLRRRTLATLAVLLHADVHAVDTDFGGYPARSGVGIWVDVDTPQEARTKVSSRGETWDLV
MSDEFEIEGRSFKAGQDHLWTALDIPDGVNAALEMYNSSNVYTKNGKLVNKIEEGPTNAT
YFNQWLEVPAMETRTLHYTAGMIQSWNKFCMQGGLIEVAAKLPGAINNVPDAEHKSVTNN
PNALGFKWVDGRKVPLPPLTPVVDGAYYPTWPGIWLMGNLGRALFAGSTTRMWPWTYNEC
DESLAPHQLISACNANPGYGLNPNQGRGAPEIDILEGGGKAISSSIQIAPGMPDQYRRKP
VVKGGPDTDNKTMETNIYCVYGKECLTPGANIPDAPTSAYKERNYKSWYQGLRYAVNSQC
PRNYAQAQGEKGYLSVKAAQDKGLINLNYWYWEEMSAAKDVHADLGLIDGKGPLHWGINY
EGRCFPIANGYVGSFLCDPDSKNPKCESPRKEGVADTNQMAKFEYQMDAISANWDINHDA
YTTFYIYQVEWVMGATGYIRWSLRDAPLFEIPAAAITDPPQDPTGKVRNPKKLMIEEPMY
IIFNVALAKAWGATPPNVGIGPCRGNATHPAPGSEAYNKTHNICDSFPMYMEVDYIRIYQ
DVSTMTLGCDPPTHPTKQWIDEHLKDYTDAKNPMTRVDGGATCNSDDDCLAIGTTVPSGR
CAERRCFCVKGYGGPRCTKFVGAKSIDTKSPNFFGPQYLYPVVLISVVALVLVLTTVVRR
RRHAAAAALAATTQSRKPTKQLDDNFDDDATPQQQQRRFYAPANSSQV

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	63	341	3.8e-67	0.7239263803680982

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	6.97e-65	38	630	98	479	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	3.16e-48	59	600	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	2.22e-09	474	599	150	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	2.56e-05	30	296	22	224	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.30e-04	62	236	2	113	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG55703.1|GH16_2	6.64e-290	29	706	25	708
UIZ28151.1|GH16	1.28e-214	28	696	30	680
UIZ27810.1|GH16	2.28e-211	28	712	65	727
UIZ28149.1|GH16	2.17e-207	2	696	1	678
UIZ27996.1|GH16	4.60e-207	28	702	30	667

PDB Hits     

H310_07910-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	6.84e-36	38	290	359	574	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	1.41e-35	15	640	309	729	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	1.83e-34	28	642	325	728	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P32486|KRE6_YEAST	3.00e-34	28	642	296	701	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|O13941|YEPB_SCHPO	2.31e-32	38	633	219	606	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000496	0.999467	CS pos: 20-21. Pr: 0.9702

TMHMM  Annotations     

Start	End
696	718