CAZyme Information: H310_05368-t26_1-p1

Basic Information

• Species: Aphanomyces invadans
• Lineage: Oomycota; NA; ; Saprolegniaceae; Aphanomyces; Aphanomyces invadans
• CAZyme ID: H310_05368-t26_1-p1
• CAZy Family: GH5
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
450		48849.97	6.3338

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AinvadansNJM9701	15416	N/A	168	15248

• Gene Location: location=KI913960:668667-670307(-)

Full Sequence      

MHLREPVEQLGGNHLDEARSESVICHDELLPWRRASAKQDVPTFDVMHHSSFLRHAIDQP
SMVKRGCHWWPLILSAVVVAAGVVIATYFTKTGLFAPSTGDPPNTTAANISANSTKPNAT
EANVTVVCDSRRMFLDDQNKCQACPEPNKTFSIFWQSHTDGCREFMKTAAFQYVTHVYWG
FAVINNKTGAVAQTFQGNDATLLDCVNAMKKKCVQQYASIGGATERANFVALNTPEKIAT
FGQTAAALVKKFGFDGIDIDDESGNILAGGNWKSNASSHVVSYLLAIRKALDDLDRKPNE
PRFGLTWDEFPTSVDSNCNDPNGDYQRCYNPTIGEVVDQVNLMMYNVERGSAYDTLLNVT
IPTVWTAAVPPSKLVLGGCVGKPGDEGACAFGASPSLPQLQRYASDGYTKYGGTMLWTGS
ADYQLNKGTTVEAMGKSSGYGASPPVPSTK

Enzyme Prediction     

EC	3.2.1.14:5	3.2.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	170	377	5.7e-20	0.5506756756756757

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119365	GH18_chitinase	3.51e-16	171	377	23	258	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
214753	Glyco_18	5.51e-16	172	377	24	232	Glyco_18 domain.
395573	Glyco_hydro_18	4.11e-15	172	377	21	219	Glycosyl hydrolases family 18.
119349	GH18_chitinase-like	7.06e-15	172	347	22	178	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.
119358	GH18_plant_chitinase_class_V	8.92e-12	171	377	23	235	The class V plant chitinases have a glycosyl hydrolase family 18 (GH18) domain, but lack the chitin-binding domain present in other GH18 enzymes. The GH18 domain of the class V chitinases has endochitinase activity in some cases and no catalytic activity in others. Included in this family is a lectin found in black locust (Robinia pseudoacacia) bark, which binds chitin but lacks chitinase activity. Also included is a chitinase-related receptor-like kinase (CHRK1) from tobacco (Nicotiana tabacum), with an N-terminal GH18 domain and a C-terminal kinase domain, which is thought to be part of a plant signaling pathway. The GH18 domain of CHRK1 is expressed extracellularly where it binds chitin but lacks chitinase activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG55900.1|GH18	5.11e-99	127	405	61	337
AIG56010.1|GH18	2.14e-59	141	445	80	387
AAX19153.1|GH18	8.09e-07	219	355	72	192
UPL00995.1|GH18	1.19e-06	219	355	130	250

PDB Hits     

H310_05368-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

H310_05368-t26_1-p1 has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000020	0.000018

TMHMM  Annotations     

Start	End
68	90