CAZyme Information: H310_03215-t26_1-p1

Basic Information

• Species: Aphanomyces invadans
• Lineage: Oomycota; NA; ; Saprolegniaceae; Aphanomyces; Aphanomyces invadans
• CAZyme ID: H310_03215-t26_1-p1
• CAZy Family: GH20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
741	KI913956|CGC2	80284.40	6.6554

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AinvadansNJM9701	15416	N/A	168	15248

• Gene Location: location=KI913956:248381-250888(+)

Full Sequence      

MKRSAVLVIVLGHGGVTSAAKGPHYDAKSGIPAWVDIDTPKQYYKATSSRGETWNLVMSD
EFNRPGRNFTAGVDPMWTAIEMADGVNAALEYYSVNMTETVMEPDGRGVFRIVTKHDDIS
FRVYNPYKTPPGFQQSRMYYRSGMLQSWNKFCMQGGLIEVMCQQPGVTGANNPDASNPQA
RVTGGIYYPTWPGVWLLGNLGRALFLSSTTRMWPWSYDVCDKNLEKDQRISACDSNPGFG
LNPNQGRGAPEIDILEGGGAAISSSVQIAPGMPDEYRPVFPADDNNAYCIYSNECKTIGA
NTPDVPSSMTVTRGHRSWYQGLRYAAHVCATSAKDVQSAVAINASLTSGITANACSLSTC
PASFDVNGDRGPVDGKAGTGLYWGINTKGQCFPVMNGYNGAYLCDPYTTNAKCTAPLHSD
GNSNGNNISPFSYQMDAISANWPVEFAAYSGYLKYQLEWVMGRQGYVRWMIDGVPLFEIP
AESLENPPQNAAMSNPKKLMIEEPLYLIFNTALSTSWGTQPPNPGKPCRGDGRDDKVNRV
CDAFPLYLKIDYIRVWQNTSTMSVGCDPASHPTRLWIDGHKSDYEDVDNPNIDVDGNAFC
TDNADCTLAGSIVTGSCSDTKRCVCSAPAVWGGPRCTSNRGSVSGSASTGSMGPPLVAVV
IISVLATAGAALIVYIRGRGKRLQLQFRGNLYANAEPMTMPQEMNLRGTVISRQGSMEEM
AASKSGGAASRTDTIRSRRVV

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	60	320	1.4e-60	0.7239263803680982

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	6.21e-63	35	592	98	481	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	3.96e-50	56	557	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	2.18e-12	35	324	30	265	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	7.85e-10	417	556	133	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	4.44e-04	443	556	135	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA25842.1|GH16_2	1.10e-250	24	637	19	641
UIZ28151.1|GH16	2.27e-245	14	637	19	660
UIZ27810.1|GH16	2.01e-241	25	676	65	726
UIZ28149.1|GH16	1.02e-238	22	655	26	679
UIZ28152.1|GH16	4.94e-238	18	656	11	667

PDB Hits     

H310_03215-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32486|KRE6_YEAST	4.98e-41	35	621	307	715	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	7.65e-38	35	621	359	767	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	2.85e-34	35	599	332	732	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	3.65e-32	35	599	336	729	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|O13941|YEPB_SCHPO	2.79e-30	35	321	219	486	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.002155	0.997840	CS pos: 19-20. Pr: 0.9617

TMHMM  Annotations     

Start	End
656	678