CAZyme Information: H257_11303-t26_1-p1

Basic Information

• Species: Aphanomyces astaci
• Lineage: Oomycota; NA; ; Saprolegniaceae; Aphanomyces; Aphanomyces astaci
• CAZyme ID: H257_11303-t26_1-p1
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
440	KI913146|CGC3	46873.85	6.7056

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AastaciAPO3	19584	N/A	465	19119

• Gene Location: location=KI913146:238073-239395(-)

Full Sequence      

MRFDFFALVSALAAYAASAAHTSKGLHPLHAAHSNAASSTTLPSTRRRILPPHTLVGYWH
NFANPSGHTFPLRDISPDWDVIVVAFGTSVGGGRVTFQVDADAGTEAEFIADIATLQGRN
KTVVLSLGGQDGAVSLANAVETTAFVDTVSTLLVKFGFDGLDLDLETGISQNLPIVTNLI
TAVKQIKQRVGSDDAFYLSMAPEHPYVQGGAGSYGSIWGAYLPIIDGLRDHLTQIHVQYY
NNGGFVYTDGRMLQEGTVDCLVGGSLMLINGFETNYGSGWRFEGLRPDQVSFGVPSGAQA
AGRGFATPDTVRNALTCLAQGVGCDTVRPSVPFPTFRGVMTWSINWDKFDRFGFSRPARA
ALDGLPQVDHVGSSDKKVVKNPTATLIPIAKPVATAAPAQAKNCGGCDNCYFAPTFACFS
GWTKAQCRSVSTFTWCGGQD

Enzyme Prediction     

EC	3.2.1.14:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	54	245	2.1e-18	0.527027027027027

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119350	GH18_chitinase_D-like	1.47e-122	53	360	1	311	GH18 domain of Chitinase D (ChiD). ChiD, a chitinase found in Bacillus circulans, hydrolyzes the 1,4-beta-linkages of N-acetylglucosamine in chitin and chitodextrins. The domain architecture of ChiD includes a catalytic glycosyl hydrolase family 18 (GH18) domain, a chitin-binding domain, and a fibronectin type III domain. The chitin-binding and fibronectin type III domains are located either N-terminal or C-terminal to the catalytic domain. This family includes exochitinase Chi36 from Bacillus cereus.
226000	Chi1	1.28e-62	29	362	9	325	Chitinase [Carbohydrate transport and metabolism].
395573	Glyco_hydro_18	4.22e-18	54	347	1	307	Glycosyl hydrolases family 18.
119349	GH18_chitinase-like	1.53e-17	55	241	1	177	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.
119356	GH18_hevamine_XipI_class_III	1.56e-14	55	348	3	269	This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG55573.1|GH18	4.32e-169	50	412	36	391
AMC37111.1|CBM12|GH18	6.22e-140	50	366	106	423
QLG89791.1|CBM12|CBM5|GH18	1.25e-131	50	366	213	530
BAK53892.1|CBM12|CBM5|GH18	7.93e-130	50	366	237	552
AXE32296.1|CBM12|GH18	3.79e-129	53	366	109	421

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4TX8_A	6.69e-126	53	372	107	427	Crystal Structure of a Family GH18 Chitinase from Chromobacterium violaceum [Chromobacterium violaceum ATCC 12472]
3N11_A	2.81e-83	55	365	9	329	Crystal stricture of wild-type chitinase from Bacillus cereus NCTU2 [Bacillus cereus],3N12_A Crystal stricture of chitinase in complex with zinc atoms from Bacillus cereus NCTU2 [Bacillus cereus]
3N15_A	7.91e-83	55	365	9	329	Crystal stricture of E145Q chitinase in complex with NAG from Bacillus cereus NCTU2 [Bacillus cereus]
3N17_A	3.14e-82	55	365	9	329	Crystal stricture of E145Q/Y227F chitinase in complex with NAG from Bacillus cereus NCTU2 [Bacillus cereus]
5KZ6_A	3.45e-82	55	366	12	333	1.25 Angstrom Crystal Structure of Chitinase from Bacillus anthracis. [Bacillus anthracis],5KZ6_B 1.25 Angstrom Crystal Structure of Chitinase from Bacillus anthracis. [Bacillus anthracis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P27050|CHID_NIACI	1.64e-71	55	365	192	514	Chitinase D OS=Niallia circulans OX=1397 GN=chiD PE=1 SV=4
sp|D4AVJ0|CHI2_ARTBC	7.16e-58	72	366	17	332	Probable class II chitinase ARB_00204 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_00204 PE=1 SV=2
sp|Q05638|CHIX_STROI	4.93e-57	39	356	248	590	Exochitinase 1 OS=Streptomyces olivaceoviridis OX=1921 GN=chi01 PE=1 SV=1
sp|A5FB63|CHIA_FLAJ1	1.47e-35	44	347	1132	1462	Chitinase ChiA OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=chiA PE=1 SV=1
sp|E9F7R6|CHI4_METRA	2.78e-31	55	347	43	330	Endochitinase 4 OS=Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) OX=655844 GN=chi4 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000343	0.999608	CS pos: 19-20. Pr: 0.9682

TMHMM  Annotations     

There is no transmembrane helices in H257_11303-t26_1-p1.