CAZyme Information: H257_00914-t26_1-p1

Basic Information

• Species: Aphanomyces astaci
• Lineage: Oomycota; NA; ; Saprolegniaceae; Aphanomyces; Aphanomyces astaci
• CAZyme ID: H257_00914-t26_1-p1
• CAZy Family: AA15
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1063		114564.77	6.7977

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AastaciAPO3	19584	N/A	465	19119

• Gene Location: location=KI913114:2564782-2567973(-)

Full Sequence      

MKVLLALVALPYATGATDFNAQAKVIVDAMTIDELIGQMTQVNIDYVIQDQNAKKVVVPS
KVADLANQRIGSYLNSPFSTSTSVSVTGWNVTEWRSAISQIQTTHKATTGHPIIYGVDSL
HGANYVQNAVLFPHQINVGATFDPAFASQMGRFAGRDTRAAGIHWIFGPCLEPARHKGWP
RIMETFGEDPTVVADMGRAIVQGIQSNNVAACFKHYIGYSGSASGKDRDPVTLSKHELLN
IFMLPFKAVIDAGIMTGMDSYIALNGVPNSANRQTSIDLLRTDLKFDGFLVSDWEEIYMM
EYFHKYATDRQDAVFKAMSNSSLDMSMVPTDTSFIGIMKTLFDSGKVSLDRIRTSAQRIV
KVKLQLNLYNDPVPGADLANAVGDSVSQSAALETAKASLVLVKNTNNVLPLDPAKFFFFT
GPSIDDIGLLCGGWTIHWQGVQGTSNFPAYGRTIQAAMSAVVGNATRAQFYQGVNIDGTW
WDINLAKQKAQAADYTVIALGERTYAELLGNSDPYELPTGLTDYVKALATTGTKIILVLV
EGRPRLLSGIADLAAAVLYAGLPCELGGEAISSVLFGSTNPSGKLPLTYPKSTDQVNLAT
PYYGRIGGNCVVGGVTTNCPVEWHFGHGLSYTSFGYSNVRLTATSLTPSLNQTVVTVTVT
NAGTTTGKESVLLFVSAPGGPETRLLKKYTKVELTPGQSTDVPFTLTPDDFGKYVNEIGQ
GLRKEATAGTYYVSLKYDTLCNAATLGPLCKAFTWNSPSTTTPVLTPAPTPAPTPAPTPA
PTPAPTPTPTPAPTSAPTPARTRTFYKLRLDAYNFVLANPSSEPVVFVADVANAPVQEWS
FDSATSQVVNRGTGRCLDAWEPQNGGAVHTWDCSATNANQYWSYEATTKQLRHKTHASYC
LDIGTTTGTKPHLWQCLASTRPDVKNQQLNLLSPSLDRLVTNTAFGSVLTAVGDAAVAFQ
PLMASSVTQLWQLDSSQLLRSTGATSKCVDAYEPQNGGAVHLWDCSATNVNQLWTHDATT
KQLRHKTHVGYCLDIGSPTGEAPHLWTCLPTTHVDLKNQVFVF

Enzyme Prediction     

EC	3.2.1.21:1	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	107	328	1.1e-58	0.9768518518518519

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185053	PRK15098	4.89e-79	30	720	45	745	beta-glucosidase BglX.
224389	BglX	9.34e-70	30	436	1	373	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
395747	Glyco_hydro_3	2.55e-54	31	362	1	316	Glycosyl hydrolase family 3 N terminal domain.
178629	PLN03080	5.38e-39	118	720	89	756	Probable beta-xylosidase; Provisional
396478	Glyco_hydro_3_C	1.48e-35	399	631	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIG56285.1|CBM13|GH3	1.44e-265	1	1059	1	1036
AIG55915.1|CBM13|GH3	3.09e-260	1	1061	1	1022
AIG55751.1|CBM13|GH3	1.37e-254	1	1061	3	1018
AIG56284.1|GH3	5.14e-212	4	755	5	740
UIZ29009.1|GH3	2.76e-205	20	754	27	761

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JP0_A	9.57e-120	30	762	24	761	Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus],5JP0_B Bacteroides ovatus Xyloglucan PUL GH3B with bound glucose [Bacteroides ovatus]
1EX1_A	8.25e-78	30	623	23	605	Beta-d-glucan Exohydrolase From Barley [Hordeum vulgare],1IEQ_A Crystal Structure Of Barley Beta-D-Glucan Glucohydrolase Isoenzyme Exo1 [Hordeum vulgare],1IEV_A Crystal Structure Of Barley Beta-d-glucan Glucohydrolase Isoenzyme Exo1 In Complex With Cyclohexitol [Hordeum vulgare],1IEW_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 2-deoxy-2-fluoro-alpha-D-glucoside [Hordeum vulgare],1IEX_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4I,4III,4V-S-trithiocellohexaose [Hordeum vulgare],1J8V_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4'-nitrophenyl 3I-thiolaminaritrioside [Hordeum vulgare],3WLH_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare],3WLJ_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 3-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLK_X Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 4-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLL_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with PEG400 [Hordeum vulgare subsp. vulgare],3WLM_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with octyl-O-glucoside [Hordeum vulgare subsp. vulgare],3WLN_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with octyl-S-glucoside [Hordeum vulgare subsp. vulgare],3WLP_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare]
3WLI_A	9.06e-78	30	623	27	609	Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare],3WLO_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare],6JG1_A Chain A, Barley exohydrolase I [Hordeum vulgare subsp. vulgare],6JG2_A Chain A, Barley exohydrolase I [Hordeum vulgare subsp. vulgare],6MD6_A CRYSTAL STRUCTURE ANALYSIS OF PLANT EXOHYDROLASE IN COMPLEX WITH METHYL 2-THIO-BETA-SOPHOROSIDE [Hordeum vulgare subsp. vulgare]
1LQ2_A	1.45e-77	30	618	23	600	Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X38_A crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X39_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare]
6JGQ_A	1.59e-76	30	623	27	609	Crystal structure of barley exohydrolaseI W434Y mutant in complex with methyl 2-thio-beta-sophoroside. [Hordeum vulgare subsp. vulgare],6JGR_A Crystal structure of barley exohydrolaseI W434Y in complex with 4'-nitrophenyl thiolaminaribioside [Hordeum vulgare subsp. vulgare],6JGS_A Crystal structure of barley exohydrolaseI W434Y mutant in complex with 4I,4III,4V-S-trithiocellohexaose. [Hordeum vulgare subsp. vulgare],6JGT_A Crystal structure of barley exohydrolaseI W434Y mutant in complex with methyl 6-thio-beta-gentiobioside. [Hordeum vulgare subsp. vulgare]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q23892|GLUA_DICDI	3.09e-121	30	711	87	791	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
sp|A7LXU3|BGH3B_BACO1	7.06e-119	30	762	46	783	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
sp|T2KMH0|PLH24_FORAG	1.45e-63	115	735	70	706	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
sp|P33363|BGLX_ECOLI	3.00e-60	30	739	45	754	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
sp|Q56078|BGLX_SALTY	1.50e-58	30	739	45	754	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000280	0.999681	CS pos: 15-16. Pr: 0.9814

TMHMM  Annotations     

There is no transmembrane helices in H257_00914-t26_1-p1.