CAZyme Information: FVEG_12368-t26_1-p1

Basic Information

• Species: Fusarium verticillioides
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium verticillioides
• CAZyme ID: FVEG_12368-t26_1-p1
• CAZy Family: GH78
• CAZyme Description: rhamnogalacturonate lyase C

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
662	CM000581.1|CGC28	74351.79	6.5498

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Fverticillioides7600	16240	334819	371	15869

• Gene Location: location=CM000581.1:3811924-3813912(-)

Full Sequence      

MAFLSLKSLALAAGLISHALAELTTAENSTHISLSNIRFAVVLAKSSGHIIDATLDGQDL
LGPLSGNSGKGPYVDCSCIPTGFWTPGSTADLRLVKGTDSTGTKYGGIIMSDTYKPTNQT
LSQYFFLRGEETGLHAFTRLTYFNASKPFLRDLGELRTLFRPNTKLWTHFSTSEGNYGPL
PDASGASTVQDATWYVGNKTDDPYVEQYSDYWTKYTLSESWRNHDVHGQFSDGSTSADGS
TFGAWLVHNTRETYYGGPLHSDLVVDGIVYNYIVSGHHGAPMPNITHGFDRTWGPQYYYF
NKGGKDATIADLRADATKYADPEWNTKFYDSIAQHVPNYTPSAKRTTYSGKVKLPKGAKK
PIIVLSENKQDFQLNVFNTKSLQYWAEIDESGGFSIPQVVDGVYRVTIYADEVFGWYIKD
DIRISKSHNKDSFTWKEENAGKELWRIGTPDKSSGEYLHGYAPDTSKPLQPEQYRIYWAK
YDFVEDFPKGVSFHVGKDQEAEDLNYVHWSFIAAKGNHLRSENYYDNVNNWTITFDLTKP
QLKNVKTATFTVQLAGVRTGNGNAKWTPVNDRFNSNLPWTVNVNGGYEDTWVIPYWRSGS
CAVRSAVACQNIEHKFEFPTERLRVGRNEFVLSLPFNASSVETALLPDTLYVQYDALRLE
VK

Enzyme Prediction     

EC	4.2.2.23:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL4	21	647	0	0.9968354430379747

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
199907	RGL4_N	9.05e-62	26	309	4	265	N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199904	RGL4_M	2.28e-27	344	425	1	82	Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199905	RGL4_C	1.15e-24	445	660	1	161	C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
405387	fn3_3	1.49e-20	355	425	1	68	Polysaccharide lyase family 4, domain II. FnIII-like is domain II of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain displays an immunoglobulin-like or more specifically Fibronectin-III type fold and shows highest structural similarity to the C-terminal beta-sandwich subdomain of the pro-hormone/propeptide processing enzyme carboxypeptidase gp180 from duck. It serves to assist in producing the deep pocket, with domain III, into which the substrate fits.
405384	CBM-like	3.30e-11	444	659	2	157	Polysaccharide lyase family 4, domain III. CBM-like is domain III of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain possesses a jelly roll beta-sandwich fold structurally homologous to carbohydrate binding modules (CBMs), and it carries two sulfate ions and a hexa-coordinated calcium ion.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKD61475.1|PL4_3	0.0	1	662	1	662
CEI70429.1|PL4_3	0.0	5	662	5	662
CEF87623.1|PL4_3	0.0	5	662	5	662
QPC62797.1|PL4_3	0.0	5	662	5	662
CZS85753.1|PL4_3	0.0	5	662	5	662

PDB Hits     

FVEG_12368-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q2U5P7|RGLC_ASPOR	0.0	5	653	5	654	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1
sp|Q5B5P1|RGLC_EMENI	0.0	14	653	14	650	Probable rhamnogalacturonate lyase C OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglC PE=3 SV=1
sp|B0XPA2|RGLB_ASPFC	4.26e-239	19	661	17	657	Probable rhamnogalacturonate lyase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=rglB PE=3 SV=1
sp|Q4WR79|RGLB_ASPFU	4.26e-239	19	661	17	657	Probable rhamnogalacturonate lyase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=rglB PE=3 SV=1
sp|B8N5T6|RGLB_ASPFN	4.11e-238	8	661	6	662	Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000272	0.999714	CS pos: 21-22. Pr: 0.9761

TMHMM  Annotations     

There is no transmembrane helices in FVEG_12368-t26_1-p1.