CAZyme Information: FPRO_00112-t41_1-p1

Basic Information

• Species: Fusarium proliferatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium proliferatum
• CAZyme ID: FPRO_00112-t41_1-p1
• CAZy Family: AA1
• CAZyme Description: related to Melibiase subfamily

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
713		80499.68	6.2746

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FproliferatumET1	16509	1227346	366	16143

• Gene Location: location=FJOF01000001:6074605-6076746(+)

Full Sequence      

MADNPVFISWQTTSLTINFVKNEFGAICLYEILPLNHRHQKSASPLFASSELPIVSVRLN
GEGNTNDKTAKSLVGGYLSSRLKYKSHQQSHDADMQRLDIHSEDEKARISVTAHLSVYRD
VPVLRSAVTIRNESKSSDVIVTQISSLTIGGLTTRSNEWNKNYALRTATNSWFREAQWRK
HSLPDIGIDKNGICELHDGHSGSQATYGLQNRGSFSTGSYLPMGILESESNSDTWAWQIE
HNGSWRWEIGDYKDSVYIAAGGPTKADHDWRHTLCPGDSFTSPPVSLTRVSGCFQDAIHA
LNDYRRRIIRPHDDNKRLPIIFNDYMNCLMGDPDEDKIEALLDPVAQSGAEYFVIDAGWY
ADDSNWWDDVGLWEPSKRRFPSGFKTLMDKIKSKGLIPGLWLEPEVVGVRSAVGERLPED
AFFHENGQRVVERGRFQLDYRHPEVRAWMTSVVDRLVVHYGAGFFKFDYNIEVIQGTDAP
GPSSAGANQLLHQRCYLDWVRSLLDKHPNLVIENCSSGAQRMDYAMLSVHSLQSTSDQQD
PILYAAIAAALPTCVLPEQSASWAYPQPEWSDELNAFTVVNSLLGRVYLSGRIDRLSPSQ
MELIIEGMDVYKMIRQHLVTAHAIWPLGLPRWHDDWISLGLETDNGLYLSVWRRSGSCSM
EIPLPRLAGSCNVQVNVLYPKRLPTQAIWNEGHDILELKFPETRCARVLHITT

Enzyme Prediction     

No EC number prediction in FPRO_00112-t41_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH36	75	621	2.3e-98	0.7747093023255814

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
269892	GH36	1.61e-85	317	612	1	298	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
307952	Melibiase	6.41e-33	279	542	1	265	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
225882	GalA	1.83e-28	49	542	51	510	Alpha-galactosidase [Carbohydrate transport and metabolism].
269893	GH27	1.07e-09	322	396	5	80	glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
407116	Glyco_hydro_36N	3.16e-06	76	183	68	181	Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCT61505.1|GH36	0.0	1	713	1	713
QGI88848.1|GH36	0.0	1	713	1	713
QGI57937.1|GH36	0.0	1	713	1	713
QGI75155.1|GH36	0.0	1	713	1	708
QKD48422.1|GH36	0.0	1	713	1	713

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2XN0_A	1.15e-26	92	616	131	634	Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN0_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN1_A Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_C Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_D Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM]
2XN2_A	1.52e-26	92	616	131	634	Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with galactose [Lactobacillus acidophilus NCFM]
6PHU_A	7.91e-25	94	615	143	642	SpAga wild type apo structure [Streptococcus pneumoniae TIGR4],6PHV_A Chain A, Alpha-galactosidase [Streptococcus pneumoniae TIGR4]
6PHW_A	3.21e-24	94	615	143	642	Chain A, Alpha-galactosidase [Streptococcus pneumoniae TIGR4],6PHX_A SpAga D472N structure in complex with raffinose [Streptococcus pneumoniae TIGR4],6PHY_A Chain A, Alpha-galactosidase [Streptococcus pneumoniae TIGR4],6PI0_A AgaD472N-Linear Blood group B type 2 trisaccharide complex structure [Streptococcus pneumoniae TIGR4]
4FNQ_A	1.57e-22	269	616	279	630	Crystal structure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|G1UB44|MELA_LACAC	5.92e-26	92	616	131	634	Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
sp|Q5ARP5|AGALG_EMENI	1.36e-25	183	542	189	552	Probable alpha-galactosidase G OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=aglG PE=2 SV=1
sp|P43467|AGAL1_PEDPE	5.31e-24	92	700	129	711	Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
sp|P27756|AGAL_STRMU	2.09e-23	68	669	101	675	Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3
sp|P43469|AGAL2_PEDPE	2.09e-23	269	665	279	672	Alpha-galactosidase 2 OS=Pediococcus pentosaceus OX=1255 GN=agaS PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000043	0.000000

TMHMM  Annotations     

There is no transmembrane helices in FPRO_00112-t41_1-p1.