CAZyme Information: FPRN_12925-t42_1-p1

Basic Information

• Species: Fusarium proliferatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium proliferatum
• CAZyme ID: FPRN_12925-t42_1-p1
• CAZy Family: GH74
• CAZyme Description: probable beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1002	FCQG01000033|CGC3	110357.58	9.3860

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FproliferatumNRRL62905	15602	N/A	348	15254

• Gene Location: location=FCQG01000033:126665-130018(-)

Full Sequence      

MKLSNLFLAALAASTHAQNVLPRGTRPSNILDSRALLQDIVTFDEHSIFIRGERVTIFSA
EIHPFRLPVASLYPDLFQKVKAMGFNMVSFYVDWALLEGKPGEFRSEGALDLRPFIDAAH
EAGIYLLARPGPYINAEVSGGGFPGWLQRVKGLLRTNATDYLEATDNYVAHVAKIIAKAQ
ITNGGPVILYQPENEYSAAQGTPFPNHDYLKYVNDQVRKAGVVVPLINNDAWPGGTGAPG
TGPGAVDIYGHDGYPVGFDCKNPYNWPKDGLPTTWHAEHEKISPNTPYSIVEFQGGAFDP
PGGYGFSNCYELTNHEFARVFYKNNIAAGVTIFNIYMTWGGTNWGNLGHSDGYTSYDYGA
AIKEDRTIAREKYSEIKLQGQFLRVSPNYAIAEASNFTTTEYTDNKNIAVTALTTKKDDA
FYVVRHADYRTTDSASYKLKVKTSTGTLTIPQLGGSLSLHRRDSKIHVVDYPVSKYKLLY
STAEVFTWKPFGDETVLVLYGGPDEVHEVAVKGQETLKVVEGDGVKTEKKKGSGVFQFKT
STKRRVVQAGSLYIYLLDRNAAYKYWVPTIPSKKSGEYGSSAMNPDAVIINGPYLVRSVA
VEGSKLSVQADFNTTTAVEIIGAPKGTSRLSINGKDTSFTKSKLGNWLVNPDIKLPTVKV
PDLKSLDWHYIDGLPEVKKDYDDSKWRTADIKKTFNSKWPLNNSVSLYSGDYGFNAGALI
FRGHFTASGSESRLKIWTFGGRAYGSSVWLDDKFLGSVAGGGNNNNDTSTYKLPKTEKGK
KHIVTVIVDNMGLNGNWVPGVDESKQPRGILDWHITSDSGKETKVTKWKITGNLGGENYK
DKFRGPLNEGGFFFERQGYHLPSPPLTSFKPGSPFKGLSKPGVSFFTAKLPLNLPSSTHD
IPLSFTFKNNTSSTGAYRAILYVNGFQYGKYVANVGPQIDFPVPEGIWNYKGDNWIGIAL
WALEKSANVDGLSLTAGVPVQTGRKPVKVVEGPKYSRRQDAY

Enzyme Prediction     

EC	3.2.1.23:18	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	49	378	1.2e-92	0.9804560260586319

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396048	Glyco_hydro_35	3.95e-104	47	378	1	313	Glycosyl hydrolases family 35.
402180	BetaGal_dom2	1.56e-72	398	564	9	180	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyzes the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C-terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N-terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, pfam01301, which is N-terminal to it, but itself has no metazoan members.
198097	BetaGal_dom2	1.83e-60	389	565	1	182	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.
166698	PLN03059	1.57e-30	41	364	30	323	beta-galactosidase; Provisional
404274	BetaGal_dom4_5	3.88e-28	853	966	1	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGI86810.1|GH35	0.0	1	1002	1	1002
CCT73835.1|GH35	0.0	1	1002	1	1002
QGJ00340.1|GH35|GH43_22	0.0	10	1002	418	1410
QGI69451.1|GH35|GH43_22	0.0	10	1002	418	1410
QKD59967.1|GH35	0.0	1	1002	1	1002

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5IFT_A	0.0	34	1002	39	1007	STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
1TG7_A	0.0	36	1002	1	971	Native structure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
5IFP_A	0.0	34	1002	39	1007	Structure Of Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
3OG2_A	0.0	24	1002	7	1003	Chain A, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]
4IUG_A	0.0	1	1002	1	1005	Crystal structure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q700S9|BGALA_PENSQ	0.0	34	1002	39	1011	Beta-galactosidase A OS=Penicillium sp. OX=5081 GN=lacA PE=1 SV=1
sp|P29853|BGAL_ASPNG	0.0	34	1002	39	1006	Beta-galactosidase OS=Aspergillus niger OX=5061 GN=lacA PE=1 SV=2
sp|Q2UCU3|BGALA_ASPOR	0.0	1	1002	1	1005	Beta-galactosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lacA PE=1 SV=1
sp|Q4WG05|BGALE_ASPFU	0.0	32	1002	33	1011	Probable beta-galactosidase E OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacE PE=3 SV=1
sp|Q4WS33|BGALA_ASPFU	0.0	34	1002	39	1006	Probable beta-galactosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000427	0.999532	CS pos: 17-18. Pr: 0.9665

TMHMM  Annotations     

There is no transmembrane helices in FPRN_12925-t42_1-p1.