CAZyme Information: FPRN_06057-t42_1-p1

Basic Information

• Species: Fusarium proliferatum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium proliferatum
• CAZyme ID: FPRN_06057-t42_1-p1
• CAZy Family: CE5
• CAZyme Description: probable glucan 1,4-alpha-glucosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
666	FCQG01000034|CGC1	71445.34	4.9769

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FproliferatumNRRL62905	15602	N/A	348	15254

• Gene Location: location=FCQG01000034:356156-358424(+)

Full Sequence      

MDLVPVGDPVPSVSINSTASLIQPIMYFVSSAFLLGSFALQSVLGRPAFDERSLVQERQS
SVDSFIKSESSVAIEQLLCNIGSDGCNSKNVATGIVIASPDTQDPDYFYTWTRDAALVFK
YVVDRFINQYDAGLQRKIQEYIASQAKLQGVSNPSGSLSDGSGLGEAKFNVDMSAFTGGW
GRPQRDGPALRATAMITYANWLIANGYTSTANDIVWPVVRNDLNYVAQYWNQTGFDLWEE
VKGSSFFTTGSQYRALIEGAALAKKLGKSGDNYSNIAPQALCFLQTYWISSGKYVDSNIN
VNDGRTGKDANSILSSIHNFDPVLNCDPATFQPCSDKALANHKAVTDSFRSWNINKGISQ
GSAVAVGRYVEDVYYNGNPWYLATLAAAEQLYDAIYVWKQQGSITVSDVSLSFFKDLVSS
ISTGTYASDSATFKSITDAVSKYADGYVAIVAKYVGTDGHLAEQFDKNDGHPLSATDLTW
SYAAFLSAADRRAGVIPPSWAGSVAAVPNQCGTNTVAGSYSSATATSFPASQTPKGGVPT
PTGTQTSTSTSSSSTSTSCPTATSVAVTFEEVVTTNFGDTIKIVGNIAALGNWDTSKAVA
LSASDYTSSNPVWKTTISLTAGQSIQYKYINIKKDGSLTWEKDPNRTYTVPKTCATKATQ
SDKWQS

Enzyme Prediction     

EC	3.2.1.3:94	1.14.99.55:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH15	79	489	7.9e-79	0.9806094182825484
CBM20	565	655	1.2e-27	0.9666666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395586	Glyco_hydro_15	1.76e-138	72	489	2	416	Glycosyl hydrolases family 15. In higher organisms this family is represented by phosphorylase kinase subunits.
99886	CBM20_glucoamylase	5.87e-51	565	664	7	106	Glucoamylase (glucan1,4-alpha-glucosidase), C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Glucoamylases are inverting, exo-acting starch hydrolases that hydrolyze starch and related polysaccharides by releasing the nonreducing end glucose. They are mainly active on alpha-1,4-glycosidic bonds but also have some activity towards 1,6-glycosidic bonds occurring in natural oligosaccharides. The ability of glucoamylases to cleave 1-6-glycosidic binds is called "debranching activity" and is of importance in industrial applications, where complete degradation of starch to glucose is needed. Most glucoamylases are multidomain proteins containing an N-terminal catalytic domain, a C-terminal CBM20 domain, and a highly O-glycosylated linker region that connects the two. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
395557	CBM_20	1.66e-35	565	656	1	91	Starch binding domain.
99883	CBM20_alpha_amylase	2.30e-28	565	664	1	94	Alpha-amylase, C-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in several bacterial and fungal alpha-amylases including the maltopentaose-forming amylases (G5-amylases). Most alpha-amylases have, in addition to the C-terminal CBM20 domain, an N-terminal catalytic domain belonging to glycosyl hydrolase family 13, which hydrolyzes internal alpha-1,4-glucosidic bonds in starch and related saccharides, yielding maltotriose and maltose. Two types of soluble substrates are used by alpha-amylases including long substrates (e.g. amylose) and short substrates (e.g. maltodextrins or maltooligosaccharides). The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
215006	CBM_2	1.79e-27	565	652	1	88	Starch binding domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGI80625.1|CBM20|GH15	0.0	1	666	1	666
QGI94229.1|CBM20|GH15	0.0	1	666	1	666
CCT67309.1|CBM20|GH15	0.0	1	666	1	666
QGI63347.1|CBM20|GH15	0.0	1	666	1	666
QKD49751.1|CBM20|GH15	0.0	1	666	10	675

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VN4_A	1.31e-281	61	666	1	599	Chain A, GLUCOAMYLASE [Trichoderma reesei],2VN7_A Chain A, GLUCOAMYLASE [Trichoderma reesei]
6FRV_A	2.18e-225	60	665	1	616	Structure of the catalytic domain of Aspergillus niger Glucoamylase [Aspergillus niger]
6FHV_A	1.00e-202	65	665	11	593	Crystal structure of Penicillium oxalicum Glucoamylase [Penicillium oxalicum 114-2]
3EQA_A	2.97e-193	60	530	1	468	Catalytic domain of glucoamylase from aspergillus niger complexed with tris and glycerol [Aspergillus niger]
1AGM_A	3.40e-192	60	530	1	467	Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. x100 to 2.4 angstroms resolution [Aspergillus awamori],1DOG_A REFINED STRUCTURE FOR THE COMPLEX OF 1-DEOXYNOJIRIMYCIN WITH GLUCOAMYLASE FROM (ASPERGILLUS AWAMORI) VAR. X100 TO 2.4 ANGSTROMS RESOLUTION [Aspergillus awamori],1GLM_A REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100 [Aspergillus awamori],3GLY_A REFINED CRYSTAL STRUCTURES OF GLUCOAMYLASE FROM ASPERGILLUS AWAMORI VAR. X100 [Aspergillus awamori]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P14804|AMYG_NEUCR	1.26e-263	26	665	1	626	Glucoamylase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=gla-1 PE=1 SV=3
sp|P69327|AMYG_ASPAW	6.37e-225	55	665	20	640	Glucoamylase OS=Aspergillus awamori OX=105351 GN=GLAA PE=1 SV=1
sp|P69328|AMYG_ASPNG	6.37e-225	55	665	20	640	Glucoamylase OS=Aspergillus niger OX=5061 GN=GLAA PE=1 SV=1
sp|P22832|AMYG_ASPUS	4.98e-224	55	665	20	639	Glucoamylase OS=Aspergillus usamii OX=186680 GN=glaA PE=3 SV=1
sp|P36914|AMYG_ASPOR	1.12e-223	55	665	23	612	Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000063	0.000004

TMHMM  Annotations     

There is no transmembrane helices in FPRN_06057-t42_1-p1.