CAZyme Information: FOZG_09442-t36_1-p1

Basic Information

• Species: Fusarium oxysporum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium oxysporum
• CAZyme ID: FOZG_09442-t36_1-p1
• CAZy Family: GH18
• CAZyme Description: beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
900		101952.66	6.7959

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FoxysporumFo47	18556	660027	365	18191

• Gene Location: location=JH717900.1:3639639-3642389(-)

Full Sequence      

MPNIMLGCNIKSATIWTLLLALRISPALCISEPNVRERIRLRDGWRFWRSESTPDNLVYD
YRPDANGTDLYVLKDWILPSGNNFIKSPDYQHERPKEEPEVDIPYIHAKFDDSDWERVQV
PHDWAISGPFYTEANPVIPGDMGRLPIQGIGWYRKSVQMTSRDLKKHVYIDIDGAMSYPM
VWLNGKLLGGWPYGYNSIRLDLTGYLKEGDNQLAIRVENPTARSSRWYPGAGIYRNVWIT
KVEQIHVAHWGTRITTRNVSSRSAEIEIEVNTASHSQRKRNVSVQTEIYENNRNSGARGQ
RVASFPHKIVQTASDTTQKTIASVKLLNPKLWGPPPTQSPHLYTAVTRLFDGRELLDEYE
TEFGIRTLLFTPDDGLHVNGQKVYIHGVNQHHDLGALGAAFNYRAAERQLETLRELGVNA
IRMAHNPPAPELLGLTDKMGFLVVDEIFDVWEIEKVPSDFHLIFKDWHEPDLRSFIRRDR
KHPSVVMWSVGNEVMEQNLHDIPASTRIAKNLKDIVYEEDNSRPVTGSLNVGFPNETLPQ
IYDIINLNYQGEGIRWGPAYEHLNRSRARPGQYDNFHGNFSNKLILGSEVGWSLSTRGTF
TFPVTPYDSSPINQSIGADYDRLEICGYELYSADAGSSADRVFKTQDEHPFVAGGFTWAG
FDYLGEPPWPEARSAYSGIIDLAGFPKERFYLYQSRWRPDLPMAHIVPHWNWPDRIGERT
PVHVFSSGDEAELFINGKSQGRVERKAGQYRFRWNETAYQPGEVRVVVYKNGKEWATQTT
QTAGEATRIKLEKDREAIDADGEDLVYLKATITDSTGRAVPSANNSVVFSVEGPGEIVAT
DNGYPADFTAFPSKTRNAFNGLVLAIVRGKYKVRGSFIVKAESKGLKSSLVTVNLNSIKG

Enzyme Prediction     

EC	3.2.1.23:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	73	810	9.8e-108	0.7021276595744681

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	7.56e-62	92	715	28	618	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
408349	Glyco_hydro2_C5	4.55e-37	789	892	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
236657	PRK10150	1.42e-35	152	548	68	470	beta-D-glucuronidase; Provisional
236548	lacZ	1.32e-29	111	525	78	485	beta-galactosidase.
236673	ebgA	3.77e-28	111	526	67	483	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKD56624.1|GH2	0.0	5	900	1	896
EAA58410.1|GH2	0.0	16	893	11	884
BCS19873.1|GH2	0.0	36	893	37	896
QYT01315.1|GH2	0.0	36	897	42	901
UKZ60430.1|GH2	0.0	32	893	37	896

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T98_A	5.69e-189	110	895	49	820	Crystal structure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
7CWD_A	1.57e-158	110	893	28	799	Chain A, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	5.81e-157	110	893	34	805	Chain A, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
6QUB_A	1.12e-133	110	900	54	881	Truncated beta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]
5DMY_A	2.52e-133	110	900	60	887	Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A7LXS9|BGH2A_BACO1	1.42e-166	110	898	66	851	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
sp|T2KM09|PLH16_FORAG	1.02e-102	61	899	40	829	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
sp|T2KN75|PLH17_FORAG	1.28e-60	110	893	50	793	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
sp|P77989|BGAL_THEP3	2.05e-56	110	898	27	741	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
sp|T2KPJ7|PLH8_FORAG	4.39e-55	100	836	64	803	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000693	0.999286	CS pos: 29-30. Pr: 0.9585

TMHMM  Annotations     

There is no transmembrane helices in FOZG_09442-t36_1-p1.