CAZyme Information: FOZG_00758-t36_1-p1

Basic Information

• Species: Fusarium oxysporum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium oxysporum
• CAZyme ID: FOZG_00758-t36_1-p1
• CAZy Family: AA11
• CAZyme Description: chitin synthase 4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1223		137017.32	8.8607

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FoxysporumFo47	18556	660027	365	18191

• Gene Location: location=JH717896.1:2077820-2081539(+)

Full Sequence      

MSLPERPGASPSYNQRNVYRNSPSRRSRPVDIETGGYHAVDNTAQHQRGRSGSSFAESIG
VNSNTESMPLSPTSPDGHSHRAGPEQPISRKRSLIRPERNRIDRDHRNYHYHKHAAHMNV
LPSSTGNDPIYEDFEASTDRSNSNLNDGGSDSSPRQRRNVSGEQEKGAAVASTMPTPDRK
KSGKIKKRSKRHSKPPKRIEEQLRPPTFWNVYCAIVTFWAPGFIMKCCGMPTRAQQRAWR
EKMGLISIILVIMAIVGFLTFGFTATVCGAPAERLRVNKVDGGNMIFHGVAYDLSKSHHP
PAQGMPLRSDGRGPNVLYDLPEKHGGQDGSFLFQNVNGRCKGLITLAKDSDVPTNKDGDL
AWYFPCTTFNQDGSSKVNLTTPYYSGYGCHTTLNSRNAFYLDLSGSADVYFTWDDIKNSS
RNLIVYSGNVLDLDLLRWFNMSQVNIPKRFEVLRDKDSEVNKAIRGRDVTRMFQSSSDKK
YAECFEEIIKVGSVDTETIGCIASKIVLYCALVLILSVVGVRFLLAIIFQWFLCRKYAPT
KTSQSSDRRKRNKQIEDWSEDIYRAPVRLPGDVGSTVYGSSDRTSKRGSFLPTTSRFSSV
GGPDVRASTGRRMPTTMASQGASNQLLTPGSIFKQGNDSRASFLRSDPYSSTPTDGPGPA
GFIHDAVVPQPPSDWMPFGFPLAHTMCLVTAYSEGEEGIRTTLDSIATTDYPNSHKVIVV
ICDGIIKGKGETMSTPDVCLGMLKDHSIPPDMVEPFSYVAVASGSKRHNMAKIYCGFYDY
GRNSRIPADRQQRVPMMVVVKCGTPDEATKSKPGNRGKRDSQIILMSFLQKVMFDERMTE
LEYEMFNGLWKVTGISPDYYEIILMVDADTKVFPDSLTHMISAMVKDPEIMGLCGETKIA
NKRDSWVTAIQVFEYFVSHHLAKSFESVFGGVTCLPGCFCMYRIKAPKGGHNYWVPILAN
PDIVEHYSENVVETLHEKNLYLLGEDRFLTTLMLRTFPKRKQVFIPQAVCKTTVPDEFMV
LLSQRRRWINSTIHNLMELVLVRDLCGTFCFSMQFIIFVELVGTLVLPAAIAFTFYVVIT
SIIHSPPQIIPLVLLGLILGLPGLLVVITAHSWSYIVWMLIYLLALPIWNFVLPTYAFWK
FDDFSWGETRKTAGEKTKKAGLEYEGEFDSSKITMKRWAEFERDKRSRSGYWGSRENVVG
GGGGGSWTSPPGHQYNDEYFSDA

Enzyme Prediction     

EC	2.4.1.16:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	661	1184	6.5e-293	0.9943074003795066

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
367353	Chitin_synth_2	0.0	659	1184	1	527	Chitin synthase. Members of this family are fungal chitin synthase EC:2.4.1.16 enzymes. They catalyze chitin synthesis as follows: UDP-N-acetyl-D-glucosamine + {(1,4)-(N-acetyl-beta-D-glucosaminyl)}(N) <=> UDP + {(1,4)-(N-acetyl-beta-D-glucosaminyl)}(N+1).
133033	Chitin_synth_C	1.72e-104	687	1035	1	244	C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin. Chitin synthase, also called UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase, catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of GlcNAc residues formed by covalent beta-1,4 linkages. Chitin is an important component of the cell wall of fungi and bacteria and it is synthesized on the cytoplasmic surface of the cell membrane by membrane bound chitin synthases. Studies with fungi have revealed that most of them contain more than one chitin synthase gene. At least five subclasses of chitin synthases have been identified.
133045	CESA_like	7.06e-23	688	946	2	165	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.
224136	BcsA	1.52e-17	860	1144	138	400	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
404513	Glyco_trans_2_3	2.40e-11	862	1083	1	194	Glycosyl transferase family group 2. Members of this family of prokaryotic proteins include putative glucosyltransferases, which are involved in bacterial capsule biosynthesis.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKD47866.1|GT2	0.0	1	1223	1	1223
AHG06595.1|GT2	0.0	1	1223	1	1222
QGI89367.1|GT2	0.0	1	1223	1	1221
CCT62022.1|GT2	0.0	1	1223	1	1221
QGI75675.1|GT2	0.0	1	1223	1	1221

PDB Hits     

FOZG_00758-t36_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q01285|CHS4_NEUCR	0.0	1	1223	1	1235	Chitin synthase 4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=chs-4 PE=3 SV=3
sp|O13394|CHS5_USTMA	0.0	90	1190	177	1305	Chitin synthase 5 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CHS5 PE=3 SV=3
sp|O13353|CHS4_MAGO7	0.0	1	1223	1	1229	Chitin synthase 4 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=CHS4 PE=3 SV=2
sp|J9VXM5|CHS3_CRYNH	0.0	73	1190	72	1200	Chitin synthase 3 OS=Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) OX=235443 GN=CHS3 PE=1 SV=1
sp|P78611|CHSD_EMENI	0.0	1	1188	1	1171	Chitin synthase D OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=chsD PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000043	0.000001

TMHMM  Annotations     

Start	End
207	224
245	267
511	533
1057	1079
1089	1108
1115	1137