CAZyme Information: FOXG_12895-t26_1-p1

Basic Information

• Species: Fusarium oxysporum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium oxysporum
• CAZyme ID: FOXG_12895-t26_1-p1
• CAZy Family: GH32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1193	CM000597.1|CGC4	130778.10	6.3657

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Foxysporum4287	21354	426428	429	20925

• Gene Location: location=CM000597.1:2804213-2808257(-)

Full Sequence      

MLKEYLVAGILLASPLLAVAQDLIPPILEKADSFVEETWSFDSSPKVIYIDDEFASVSDK
DGLTLIPPTAHEFATTFQDDLAKITGSKWTLKRVDKLSNNASGISLGSYTGESSDLRYEN
GKSTSEGYEIIINSNRVFIGGSGARGMWWGTRTFLQLLLAGNGTITSTLGRDAPAYATRG
YMLDAGRKWYSKDFLKELCSYASFFKMNEFHYHLSDNYPLNRGKNDSWRDVYSHFSLLPE
DKSLRGILHGRENETLSRDDFMELQSHCASRGVTVIPEIEAPGHSLYLTKWKPELALAKK
DLLNLTHPETLPTVQSIWEEFLPWFNTKEVHIGADEYDAELADDYITFVNKMSRFINSTA
NKRSRIWGTHEPSKRNQTIDKNVIIQHWQYGQSDPVQLVKDGYDVINSEDWWAYTSLKND
HMPILPARYPQFFNESRVFNFADKEEWQWTPADFNPVNTSLQLKSDEKRLRGATLAAWND
NGPDATTQLEAYYSMRRGIAVVGGRAWSGSRGPKLLEETSDTSVDFYSLRAPDQNLDRVL
SLGANGTLVSWTRSGGDGKEVHLGHGSKGMNYTLTLSVTGPFTLSGPDNTLSLDKSGNMV
FTADGWEYPLRKVTNDDALDLDPGAPGRIWVNTSSTHKPVKVSAPVNITLVTDILHGTVV
FRDSEVLGRFEVFVYGGRNTQFSWSQMAFVAPLDKIEGGLERLELSGSSNFTGASETGGE
EPTDVPQGSDAARCLASSAFITLGLVVGGLLLNQNGSQICIGLKIDTVYSGLQGIQSSPL
QPLTYRRRAVMDLWQVGQRKYNDEGRGGGTQSPVIVCGSVGGFQEGRHRPGMRPGCLFSR
LRSILDSPRDSDYEPLSLEADRGESGVNANSWDEGGLTALHAAIHCSGPTACLHIIDSGG
TVEYLTRLAAKLLQHGADANAVDSSGNPTLYTAILNIACSLQAWERGLMDVLLQGGAGAN
ALDGNGASALHLAATHGVHAGVIISGLLRHGADPRTRCRDGRCVLHYLARFRRPPDVSKQ
LRNLGLDPNARDEHGQTPLLHATVAGLVKMVRLLCESGIGVNTPIFDDDKTAFFCAVRLK
TVDAVQVLLQYGADLHHRGHSGRTVLHYHEAAYKAMSNGSADAARINKLLIACGANVNAK
RGYCRQGHGVSVLDAGGITRAQGEFALFLMANGALRFRYNEESMLSLVSCALE

Enzyme Prediction     

No EC number prediction in FOXG_12895-t26_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	169	509	1.9e-60	0.9792284866468842

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119334	GH20_DspB_LnbB-like	5.63e-104	178	509	2	326	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
119331	GH20_hexosaminidase	2.97e-38	179	508	2	303	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
395590	Glyco_hydro_20	3.18e-34	176	509	1	345	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
119333	GH20_chitobiase-like	2.11e-26	176	478	1	316	The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
226056	Chb	2.74e-24	124	508	204	614	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKD58866.1|GH20	0.0	1	1177	1	954
QGI98311.1|GH20	0.0	1	752	1	751
QGI84655.1|GH20	0.0	1	752	1	751
QGI67429.1|GH20	0.0	1	752	1	751
CCT71670.1|GH20	0.0	1	752	1	751

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JQF_A	5.55e-33	126	518	160	557	Crystallization analysis of a beta-N-acetylhexosaminidase (Am2136) from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835]
3GH4_A	4.30e-22	126	453	122	449	Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 [Paenibacillus sp.],3GH5_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with GlcNAc [Paenibacillus sp.],3GH7_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with GalNAc [Paenibacillus sp.],3SUR_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with NAG-thiazoline. [Paenibacillus sp. TS12],3SUS_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with Gal-NAG-thiazoline [Paenibacillus sp. TS12],3SUT_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with PUGNAc [Paenibacillus sp. TS12],3SUU_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with Gal-PUGNAc [Paenibacillus sp. TS12],3SUV_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with NHAc-DNJ [Paenibacillus sp. TS12],3SUW_A Crystal structure of beta-hexosaminidase from Paenibacillus sp. TS12 in complex with NHAc-CAS [Paenibacillus sp. TS12]
4H04_A	1.34e-21	107	511	88	478	Lacto-N-biosidase from Bifidobacterium bifidum [Bifidobacterium bifidum JCM 1254],4H04_B Lacto-N-biosidase from Bifidobacterium bifidum [Bifidobacterium bifidum JCM 1254],4JAW_A Crystal Structure of Lacto-N-Biosidase from Bifidobacterium bifidum complexed with LNB-thiazoline [Bifidobacterium bifidum JCM 1254],4JAW_B Crystal Structure of Lacto-N-Biosidase from Bifidobacterium bifidum complexed with LNB-thiazoline [Bifidobacterium bifidum JCM 1254],5BXP_A LNBase in complex with LNB-LOGNAc [Bifidobacterium bifidum JCM 1254],5BXP_B LNBase in complex with LNB-LOGNAc [Bifidobacterium bifidum JCM 1254],5BXR_A LNBase in complex with LNB-NHAcDNJ [Bifidobacterium bifidum JCM 1254],5BXR_B LNBase in complex with LNB-NHAcDNJ [Bifidobacterium bifidum JCM 1254],5BXS_A LNBase in complex with LNB-NHAcCAS [Bifidobacterium bifidum JCM 1254],5BXS_B LNBase in complex with LNB-NHAcCAS [Bifidobacterium bifidum JCM 1254],5BXT_A LNBase in complex with LNB-NHAcAUS [Bifidobacterium bifidum JCM 1254],5BXT_B LNBase in complex with LNB-NHAcAUS [Bifidobacterium bifidum JCM 1254]
6EZR_A	3.22e-18	80	478	169	582	Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZR_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZS_A Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6EZS_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6K35_A Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi],6K35_B Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi]
6EZT_A	2.91e-17	80	478	166	579	Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi],6EZT_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|B2UPR7|H2136_AKKM8	3.71e-35	126	518	182	579	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1
sp|P96155|HEX1_VIBFU	9.25e-19	113	427	194	540	Beta-hexosaminidase OS=Vibrio furnissii OX=29494 GN=exoI PE=1 SV=1
sp|P49008|HEXA_PORGI	1.47e-14	126	336	111	335	Beta-hexosaminidase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=nahA PE=3 SV=2
sp|Q9HGI3|HEXA_EMEND	1.48e-14	171	520	180	558	Beta-hexosaminidase OS=Emericella nidulans OX=162425 GN=nagA PE=1 SV=1
sp|P43077|HEX1_CANAX	5.36e-14	77	511	62	509	Beta-hexosaminidase OS=Candida albicans OX=5476 GN=HEX1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000618	0.999363	CS pos: 20-21. Pr: 0.9723

TMHMM  Annotations     

There is no transmembrane helices in FOXG_12895-t26_1-p1.