CAZyme Information: FOXG_12492-t26_1-p1

Basic Information

• Species: Fusarium oxysporum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium oxysporum
• CAZyme ID: FOXG_12492-t26_1-p1
• CAZy Family: GH30
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
434		47540.27	5.0024

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Foxysporum4287	21354	426428	429	20925

• Gene Location: location=CM000591.1:777115-778685(-)

Full Sequence      

MTLLSVCLTTLAVMSQVQRTVFTSAIQQPGSPTIHERENQYVNSVYFVNWGIYGRNFHPK
DLPVSSLTHVLYSFLNIKPDGTVYSGDVYADLGKRYEGDSLREDGENAYGCVKQLYLLKK
ENRKLKTMLSIGGWTWSTNFPAVARSLPSRSRFAKSAVSLMKDWGFDGLDIDWEYPSNSE
DAENMVLLLQAVRDELDAYAAAFAPNHHFQLSIAAPAGFEHYSKLQISRLATVVDHLNLM
AYDYVSTSTASHNANLYANDHITGATPFNTDDAVRAYIQAGAPSEKLILGIPIYGRSFIG
TSGLGELSSGAGLAGSWEDGVWDYKALPKVGATVFFDDKAKASFSYDSSVLELVSFDTPQ
AVQEKVFYLKRLGLGGSMFWEASGDKGLGSESLIQSSASCLGSLDATENWLHYPDSRYQN
IASGMGVGDAESLT

Enzyme Prediction     

EC	3.2.1.14:58

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	41	389	1.1e-75	0.9493243243243243

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119365	GH18_chitinase	3.95e-121	45	385	3	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
214753	Glyco_18	7.49e-120	46	385	5	334	Glyco_18 domain.
395573	Glyco_hydro_18	2.91e-92	45	385	4	307	Glycosyl hydrolases family 18.
225862	ChiA	1.19e-89	35	386	32	424	Chitinase, GH18 family [Carbohydrate transport and metabolism].
119351	GH18_chitolectin_chitotriosidase	5.77e-84	46	380	4	336	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UPL00995.1|GH18	1.46e-189	39	424	39	424
QGI87279.1|GH18	1.98e-184	34	425	23	414
QGJ00792.1|GH18	1.98e-184	34	425	23	414
QGI69904.1|GH18	1.98e-184	34	425	23	414
CCT74476.1|GH18	1.62e-183	34	425	23	414

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EPB_A	5.59e-170	23	425	18	422	Structure of Chitinase 42 from Trichoderma harzianum [Trichoderma harzianum]
6YLJ_A	7.44e-168	23	425	18	422	Chain A, Endochitinase 42 [Trichoderma harzianum],6YN4_A Chain A, Endochitinase 42 [Trichoderma harzianum],7AKQ_A Chain A, Endochitinase 42 [Trichoderma harzianum]
3G6L_A	5.19e-159	23	425	3	405	The crystal structure of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea [Clonostachys rosea],3G6M_A crystal structure of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea in complex with a potent inhibitor caffeine [Clonostachys rosea]
6IGY_A	5.09e-135	40	425	15	403	Crystal structure of Aspergillus niger chitinase B [Aspergillus niger]
1WNO_A	9.28e-132	41	425	5	393	Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407 [Aspergillus fumigatus],1WNO_B Crystal structure of a native chitinase from Aspergillus fumigatus YJ-407 [Aspergillus fumigatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32470|CHI1_APHAL	9.10e-178	34	425	31	422	Chitinase 1 OS=Aphanocladium album OX=12942 GN=CHI1 PE=1 SV=2
sp|E9ERT9|CHI1_METRA	2.69e-177	11	425	9	423	Endochitinase 1 OS=Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) OX=655844 GN=chit1 PE=3 SV=1
sp|A6N6J0|CHI46_TRIHA	7.38e-169	12	425	10	429	Endochitinase 46 OS=Trichoderma harzianum OX=5544 GN=chit46 PE=1 SV=1
sp|P48827|CHI42_TRIHA	6.67e-168	23	425	18	422	Endochitinase 42 OS=Trichoderma harzianum OX=5544 GN=chit42 PE=1 SV=1
sp|O14456|CHI1_METAN	2.70e-167	11	425	9	422	Endochitinase 1 OS=Metarhizium anisopliae OX=5530 GN=chit1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.002605	0.997341	CS pos: 19-20. Pr: 0.7929

TMHMM  Annotations     

There is no transmembrane helices in FOXG_12492-t26_1-p1.