CAZyme Information: FOMG_07650-t38_1-p1

Basic Information

• Species: Fusarium oxysporum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium oxysporum
• CAZyme ID: FOMG_07650-t38_1-p1
• CAZy Family: GH13
• CAZyme Description: beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
834		94752.08	5.5308

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_Foxysporum26406	20033	1089452	372	19661

• Gene Location: location=JH659333.1:38477-41303(+)

Full Sequence      

MMPPIAQRTLLEKGWSFKKVTDGHDAWKPVARVPTVAHIDLIDNGIIPDPFLNMNELEVE
WVGETAWTYRTTFDSPSANSRSVYLVFEGLDTFAQVKLNDVIILESDNMFLSHRVDVTNK
LSNEGPNVLSIDFDSALLKAREIKEQYPDHRWELFNGEAGRLVVRKAQYHWGWDWGPVLN
TAGPWKPVWLEVSSAHINDFLLKYTVSPDLEKIQGTVEVDIEGLYDTANVSINFQDDAVY
TTTAAKSSDGRLVVPFAIDQPKLWYPAGYGHQPQYSVSVSIVKDSQKLDAKTKKTGFRRS
ELVQKGDSHGKSFFFRINNIDIFCGGSCWIPADNLLPRITPAKYRDWLKLMIEGNQIMTR
VWGGGIYEDDAFYDCCDEMGILVWQDFMFGCGNYPVGPSLIKSIRKEAVQNVGRLHHHPS
IVIYAGNNEDYQVQEQAGLEYNPDDKDPSSWLKSSFPARYYYEYLLPEVVNEVSPGMIYW
PGSPFSGGKDTADKTTGDLHQWNVWHGTQEKYQVFDRLGGRFNSEFGMEAFPALSTIKHC
ITEDCDRFPQSQMMDFHNKADGHERRIATYVVENFRPLPDLESHIYLTQVCQSEAMTFAY
RSWRRQWGDDRRCGGVLVWQMNDCWPAISWSIVDYFLTKKPAYYSIRRALKPIAAGVQRQ
HHDWSVVHARPAKTSSFEVWVASSKQQEVTVTVEIRFISIKTGRDIRDKIVKANTVLVPN
GTTDILTGQIDNAKDEPHVISVSVLQSGVCVSQDVDWPQPLKYLDFSDRGVEIQVGPDGY
QLTANKPTKGLVFEELPGVSLEDNCIDLMPGEVVTVKARGSETLSGVPKYRYLY

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	7	706	3.2e-97	0.6648936170212766

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.03e-80	45	647	45	627	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	5.34e-13	675	763	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	1.10e-08	195	298	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKD57951.1|GH2	0.0	2	833	1	832
CCT70824.1|GH2	0.0	2	833	1	832
QGI83791.1|GH2	0.0	2	833	1	832
QGI66550.1|GH2	0.0	2	833	1	832
QGI97440.1|GH2	0.0	2	833	1	832

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	9.29e-146	11	658	29	661	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	1.51e-115	10	812	10	810	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.58e-115	10	812	12	812	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.62e-115	10	812	12	812	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	4.22e-115	10	812	10	810	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WAH4|MANBB_ASPFU	0.0	7	833	5	843	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
sp|A2QYN2|MANBB_ASPNC	0.0	2	833	1	842	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
sp|A1D911|MANBB_NEOFI	0.0	7	833	5	843	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
sp|B8NW36|MANBB_ASPFN	0.0	11	833	9	842	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1
sp|A1CGA8|MANBB_ASPCL	0.0	7	833	5	843	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000044	0.000000

TMHMM  Annotations     

There is no transmembrane helices in FOMG_07650-t38_1-p1.