CAZyme Information: FOIG_07925-t36_1-p1

Basic Information

• Species: Fusarium odoratissimum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium odoratissimum
• CAZyme ID: FOIG_07925-t36_1-p1
• CAZy Family: GH154
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
344	JH658282.1|CGC2	38105.62	5.2008

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FodoratissimumNRRL54006	16975	1089451	341	16634

• Gene Location: location=JH658282.1:1360513-1361596(+)

Full Sequence      

MIFPLTHLLVTARLVSGACECGYSIKNPQGDSPIVFTDRLETKFSQLHNISEGHDWVAQN
FSVSAADGRGNFSKAFLPTNVNTRGTKSGDDSGLELRVRSVVNKDDAIPSSEVDTARLDL
HWGSFRAGMKLTDVKGTCAAFFWYFNDTQEIDIEFLSQEFDHDNGIYPINLVVQSKKSLE
AGYDASKTGSFKRVRLDFDPTDAFHEYRFDYLPGQVLFYADGKLLARMKGGDMPSSGGHL
ILQHWSNGNPLWSGGPPKEDATVTVSFVKAYFNSSNSERQSQLVQQCHKSSEETSGCAIY
DSDNDEIDNAAASTRRVAQENFVCVLVVGLIMPFLQLSHIFLVV

Enzyme Prediction     

No EC number prediction in FOIG_07925-t36_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	86	271	6.1e-90	0.9583333333333334

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	4.36e-46	55	271	12	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	4.81e-13	65	269	15	212	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
395585	Glyco_hydro_16	2.93e-07	121	255	32	160	Glycosyl hydrolases family 16.
225182	BglS	1.47e-05	94	286	89	274	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185685	GH16_XET	2.36e-04	123	223	49	142	Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QKD58555.1|GH16_23	1.30e-203	1	344	1	298
CCT71404.1|GH16_23	5.81e-184	1	344	1	298
QGI84375.1|GH16_23	5.81e-184	1	344	1	298
QGI98026.1|GH16_23	5.81e-184	1	344	1	298
QGI67140.1|GH16_23	5.81e-184	1	344	1	298

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CPN_A	4.15e-08	122	254	14	136	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1MAC_A	4.36e-08	122	254	70	192	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1BYH_A	4.46e-08	122	254	72	194	MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
1CPM_A	4.46e-08	122	254	14	136	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1AXK_A	1.22e-07	122	254	14	136	Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis],1AXK_B Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P23904|GUB_PAEMA	2.93e-07	122	254	95	217	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
sp|P45797|GUB_PAEPO	9.70e-07	122	254	96	218	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.775205	0.224797

TMHMM  Annotations     

There is no transmembrane helices in FOIG_07925-t36_1-p1.