CAZyme Information: FGRAMPH1_01T26583-p1

Basic Information

• Species: Fusarium graminearum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium graminearum
• CAZyme ID: FGRAMPH1_01T26583-p1
• CAZy Family: GT50
• CAZyme Description: dolichyl-P-Man:Man(5) c(2)-PP-dolichyl mannosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
432		49221.07	9.9131

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FgraminearumPH-1	14145	229533	2	14143

• Gene Location: location=HG970335:5784410-5785818(-)

Full Sequence      

MADPAPGALARGTRFVRNVLTGQHALSKLIPVALWLADAVGTSLIIWKVPYTEIDWEAYM
QQVSQFISGERDYTKIEGGTGPLVYPAAHVYTFTGLYHITNEGENIFLAQQIFGVLYMAT
LAVVMLCYWKAKVPPYMFVFLIASKRLHSLFVLRCFNDCFAVFFLWLSIYFFQRRNWTFG
SLAYTWGLGIKMSLLLVLPAIGVILLLGRGFWPGLRLAWLMAQVQFAIGIPFIMKNSRGY
AARAFELSREFKFEWTVNWRMLGEEVFLSKSFAIFLLACHVTALLVFISQRWLQPTGRPL
SAMIPSFLQLKSPFTLQEQLRISHYVTPEYVMTTMLSANVIGLLFARSLHYQFYAYLAWA
SPYLIWRATEDPFIVLLIWAAQEWAWNVFPSTDLSSRVTVGAMLATVVLAYRGTARLAVP
PSQARKIEAKNK

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	33	396	1.7e-143	0.9917582417582418

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	32	396	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CEF85199.1|GT58	1.19e-316	1	432	1	432
CZS74204.1|GT58	1.19e-316	1	432	1	432
QPC72767.1|GT58	6.91e-314	1	432	1	432
QPC63891.1|GT58	7.19e-309	1	432	363	794
CEI40371.1|GT58	9.64e-305	1	432	1	432

PDB Hits     

FGRAMPH1_01T26583-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9C1K8|ALG3_NEUCR	2.69e-176	15	407	21	415	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=alg-3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	1.07e-132	36	407	34	396	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|Q2U6A4|ALG3_ASPOR	5.01e-132	27	407	17	388	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|A1DDZ3|ALG3_NEOFI	2.27e-127	36	407	26	388	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=alg3 PE=3 SV=1
sp|A1CBE6|ALG3_ASPCL	2.65e-125	36	408	88	451	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999939	0.000078

TMHMM  Annotations     

Start	End
107	129
150	172
185	207
212	234
266	288