CAZyme Information: FGRAMPH1_01T19835-p1

Basic Information

• Species: Fusarium graminearum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium graminearum
• CAZyme ID: FGRAMPH1_01T19835-p1
• CAZy Family: GH47
• CAZyme Description: Chitinase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1461		155450.28	4.5090

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FgraminearumPH-1	14145	229533	2	14143

• Gene Location: location=HG970334:4751122-4755561(+)

Full Sequence      

MKRPIPRSAITLMMVFVALMIPYTSALPSLRIMPLGDSITKGNGSKDQKGYRNRLREKLI
SRGTSVDMVGSLKHPPTGMADNDHEGHSGKVLAQINTYWKLSIAAQPNVVLVHAGTNNMD
LEEDLEGSPQILESIIDGLFQYCPDTTVLVAPVIWANKPRMQQNTDRFNPQVIAMIEKRQ
NAGKHILEVPIDITADDLWDEKHPNDSGYEKMANAWLKAILEADKRGWLKTPTKIDSAGL
PGVGLGIGGGAGGAQEEIVGKIWKKQGTVFKGFRTWESVGSIRGTAANATREKVILADLN
GDGITDYILADEDGTMRAWINGGRPNDWKSIGYINPDWKSVKGNMIRLADVDNDGKADLI
AIYENGAAKVWKNVDNGKKFESMDSKWATGLESRDKVSFQDIDGDGYADYVIVYSGGAVK
WARNTHNNGKDSSKKNWEAEVTIAPGPSGIPEGSTRIQDIDGDGKADYLVVYDGGAVKAF
RNTLKEGGRNWDDLGTVAPGIGGVTGDMIRFADMDGDGLADFLAVADDGSIRMWKNLGIT
GTKGQSIRFADLTGEGRDDLISVDAKGRARAWINKGDGQWEAIGEIAPGLDEDLSDARIE
FVDVNSDKRADYLIIYGGGAVKAYLNNGNLPDPGDRRIWQDGIVISPGVGEPGSKIRFAD
LDGDGYADFLILYDGGAVKYWQNNKNIPPRNGGRIWKEGIVVATGVGEPGSKIRFADLTG
DGKADYIVQYDGGAARGYRNNGKIPIGEGRKWNDMGTIAGGVSPQGPVRYADINGDRKAD
YLVVSDGGAVNAYMNNHDWAPKLPDKPDQPIPSEPGEGGGGGGGDGGSGGGGGGDDNDGG
SGGDGGDDAVYIDPTIWDSKNPTAGCQPPCTLVLPPWPLSSKTTISFPVLTETLKETWPE
TTDGVIKYRTTTITVLITLPPLTTSEIGVSNIIITGSTSKSIPVKGSVIPSPVTLTEPTR
GITYTYKPGPMPTGEDKIGPPPGIIPGIIVTAGPPGPICKSGCGNLCLFGCAPGGGGGGG
GGGSIGCIGGGCPGPGGNCVGAGCEKDKDKDDEDDNDDDEDEEDCATETNTACHQVCTTK
PCATVCNTYLGCDCTTSRATDYWVSCQSSSCTTTSSEVITGCFLTATATTTEASCPLITL
DHENDLLGDDSNIYGNLGTTYKTTFSATVIVSKRAYPVNDGYVTVEKTAYPIPDVDTATR
TKMRGTSAIILPSLVGVGFSITNKDFPVGTTSYPKATTTVGESTSTTSEEPSPTSIAYPT
NTLINQEDGEGYCFDDSSSYEEFTIEDAQRVIGSFCDSSYTLDPKNDFGHNSALENDGYT
VIVSAKWAPDQEGCGDKEPFSFSEGAINHDLCPRAWNTNFYCEDPENGDNEVSYGGAYVL
DLPESGGCLLLSLYAYDRSTMRLKALPKGAESMIPSRMNVTRVGHEPKWKDSNRPGIKQV
WPVAEGSSPGGRKTLKPARGD

Enzyme Prediction     

No EC number prediction in FGRAMPH1_01T19835-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE3	30	220	1.7e-52	0.9948453608247423

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
238871	XynB_like	5.44e-55	30	220	1	157	SGNH_hydrolase subfamily, similar to Ruminococcus flavefaciens XynB. Most likely a secreted hydrolase with xylanase activity. SGNH hydrolases are a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.
404371	Lipase_GDSL_2	5.81e-15	35	211	2	176	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
238141	SGNH_hydrolase	5.06e-11	35	219	4	187	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.
404411	VCBS	1.20e-07	660	728	1	61	Repeat domain in Vibrio, Colwellia, Bradyrhizobium and Shewanella. This domain of about 100 residues is found in multiple (up to 35) copies in long proteins from several species of Vibrio, Colwellia, Bradyrhizobium, and Shewanella (hence the name VCBS) and in smaller copy numbers in proteins from several other bacteria. The large protein size and repeat copy numbers, species distribution, and suggested activities of several member proteins suggests a role for this domain in adhesion (TIGR).
404411	VCBS	4.48e-07	459	524	1	61	Repeat domain in Vibrio, Colwellia, Bradyrhizobium and Shewanella. This domain of about 100 residues is found in multiple (up to 35) copies in long proteins from several species of Vibrio, Colwellia, Bradyrhizobium, and Shewanella (hence the name VCBS) and in smaller copy numbers in proteins from several other bacteria. The large protein size and repeat copy numbers, species distribution, and suggested activities of several member proteins suggests a role for this domain in adhesion (TIGR).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QPC79577.1|CE3	0.0	1	1461	1	1471
QKD52243.1|CE3	0.0	7	1452	7	1446
CCT63634.1|CE3	0.0	7	1452	7	1467
UPK99830.1|CE0	0.0	7	1460	7	1463
QPC65629.1|CE3	0.0	7	1400	7	1379

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VPT_A	2.26e-25	29	222	5	197	Chain A, LIPOLYTIC ENZYME [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9RLB8|CESA_RUMFL	4.49e-12	24	230	38	265	Multidomain esterase OS=Ruminococcus flavefaciens OX=1265 GN=cesA PE=1 SV=1
sp|P15329|GUNX_ACETH	1.64e-06	85	222	7	139	Putative endoglucanase X (Fragment) OS=Acetivibrio thermocellus OX=1515 GN=celX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000310	0.999650	CS pos: 26-27. Pr: 0.9755

TMHMM  Annotations     

Start	End
5	27