CAZyme Information: FGRAMPH1_01T19521-p1

Basic Information

• Species: Fusarium graminearum
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium graminearum
• CAZyme ID: FGRAMPH1_01T19521-p1
• CAZy Family: GH43|CBM91
• CAZyme Description: rhamnogalacturonate lyase precursor

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
670	HG970334|CGC21	75766.59	6.7421

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FgraminearumPH-1	14145	229533	2	14143

• Gene Location: location=HG970334:4346369-4348589(+)

Full Sequence      

MKFRKLSTAFLVSLFYVPQLVAAALNATETDTQLVISNDRLYAAVQKKGGAIVKLTLDGT
NLLGSPSGSTGIGPYLDCYCTPKGFWTPGSVTPKYKLFKGKDAKGKDYGGIVMSDTYTET
GQVLEQYWFLRDGETGLHTFSRVAYHNEEQPFLRNLQELRTLFRPNNDMWTHLLTNTKHY
APLPGKEAKEKQVVVQDATWYMGNTPNDPYVKQEADYFTKYTFQDNWRDIDAYGLFADGS
KTEDGDAYGAWLVMNTKDTYFGGPLHSDLVVDGILYNYISSNHHGDQTPNITNGFDRTFG
PQYFHFNRFPGTTDILKAQADAAQYADPEWNADFYDSIAKHVPNYVPTKGRGKFEVKVDL
PKGAKNAIAVLAQSGVDFQDNVFDTKAYQYWANLDESGRATIPRVKSGTYRLTVYADNIF
GQYTQDKIKVSPGKTEKKNVRWREESAGKELWRVGTPDKTSGEYRHGFEPDTSKPLQPEQ
YRIYWANWDFVKDFPDGVNFKVGESDVGKDLNYVHWSVFGGKGNYPRPEQYVGNGDVNNW
TVAFDLKESQFKHKKRATFTVQLAGAKTAAGNTDVYNASEPHSNLKYTVNINGKDLEPWV
IPYYQSSSCAVRSSVSCYNLAHKFEFDAKWLKKGENEMVLSLPYNGTNYESAVLPTSVYV
QYDALRLEIE

Enzyme Prediction     

EC	4.2.2.23:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL4	23	655	0	0.9968354430379747

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
199907	RGL4_N	2.18e-49	28	308	4	258	N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199904	RGL4_M	6.03e-33	350	435	1	86	Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
199905	RGL4_C	2.16e-31	452	668	1	161	C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
405387	fn3_3	1.31e-26	361	435	1	72	Polysaccharide lyase family 4, domain II. FnIII-like is domain II of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain displays an immunoglobulin-like or more specifically Fibronectin-III type fold and shows highest structural similarity to the C-terminal beta-sandwich subdomain of the pro-hormone/propeptide processing enzyme carboxypeptidase gp180 from duck. It serves to assist in producing the deep pocket, with domain III, into which the substrate fits.
405384	CBM-like	8.64e-16	451	667	2	157	Polysaccharide lyase family 4, domain III. CBM-like is domain III of rhamnogalacturonan lyase (RG-lyase). The full-length protein specifically recognizes and cleaves alpha-1,4 glycosidic bonds between l-rhamnose and d-galacturonic acids in the backbone of rhamnogalacturonan-I, a major component of the plant cell wall polysaccharide, pectin. This domain possesses a jelly roll beta-sandwich fold structurally homologous to carbohydrate binding modules (CBMs), and it carries two sulfate ions and a hexa-coordinated calcium ion.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CEF87176.1|PL4_3	0.0	1	670	1	670
CZS84860.1|PL4_3	0.0	1	670	1	670
QPC79455.1|PL4_3	0.0	1	670	1	670
QPC62043.1|PL4_3	0.0	1	670	1	686
CEI69636.1|PL4_3	0.0	1	670	1	670

PDB Hits     

FGRAMPH1_01T19521-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AZ85|RGLB_EMENI	1.23e-280	23	670	18	660	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2
sp|Q2U5P7|RGLC_ASPOR	6.25e-270	23	661	21	654	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1
sp|A1D144|RGLB_NEOFI	1.58e-268	23	670	19	658	Probable rhamnogalacturonate lyase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=rglB PE=3 SV=1
sp|Q0C7K7|RGLB_ASPTN	1.38e-267	22	669	18	659	Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rglB PE=3 SV=1
sp|A5ABH4|RGLB_ASPNC	5.68e-266	20	658	16	651	Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000272	0.999689	CS pos: 23-24. Pr: 0.8106

TMHMM  Annotations     

There is no transmembrane helices in FGRAMPH1_01T19521-p1.