CAZyme Information: FFUJ_02016-t31_1-p1

Basic Information

• Species: Fusarium fujikuroi
• Lineage: Ascomycota; Sordariomycetes; ; Nectriaceae; Fusarium; Fusarium fujikuroi
• CAZyme ID: FFUJ_02016-t31_1-p1
• CAZy Family: AA7
• CAZyme Description: related to Melibiase subfamily

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
713	HF679023|CGC1	80264.42	6.6547

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_FfujikuroiIMI58289	15099	1279085	286	14813

• Gene Location: location=HF679023:203742-205883(+)

Full Sequence      

MADNPVFISWQTTSLTINFVKNEFGAICLYEILPLNHRHQKSASPLFASSELPLVSVRLN
GEGNTNDKTAKSLVGGYLSSRLRYKSHQQSHDGDMQRLDIHSEDERSGISVTTHLSVYRD
VPVLRSAVTIRNESKSSDVIVTQLSSLTIGGLTTRSNEWNKDYILRTATNTWFREAQWRK
HSLPDIGIDKNGICELGDGHSGSQATFGLQNRGSFSTGSYLPMGILESESNSDTWAWQIE
HNGSWRWEIGDYKDSVYVAAGGPTKADHDWRHTLRPGDSFTSPPVSLTRVSGCFQDAIRA
LNDYRRRIIRPHDDNKRVPIVFNDYMNCLMGDPDEDKIEALLDPVAQSGAEYFVIDAGWY
ADDSNWWDDVGLWEPSKRRFPSGFKTLMDKIKSKGLIPGLWLEPEVVGVRSVVGERLPED
AFFHENGQRVVERGRFQLDYRHPEVRAWMTSVADRLVVHYGAGFFKFDYNIEVIQGTDAP
GPSSAGANQLLHQRCYLDWVRSLLDKHPNLVIENCSSGAQRMDYAMLSVHSLQSTSDQQD
PILYAAIAAALPTGVLPEQSASWAYPQPEWSDELNAFTVVNSLLGRVYLSGRLDRLSTSQ
MELIIEGMDVNKMIRRHLVTAHAIWPLGLPRWHDDWISLGLETDNGLYLSVWRRGGSCSK
KIPLPRLAGSGKVQVNVLYPKRLPTQAIWNKGQDALELKLPETRCARVLHITT

Enzyme Prediction     

No EC number prediction in FFUJ_02016-t31_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH36	75	621	1.7e-96	0.7747093023255814

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
269892	GH36	2.92e-81	319	609	3	295	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
307952	Melibiase	5.45e-33	279	542	1	265	Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
225882	GalA	2.34e-28	49	542	51	510	Alpha-galactosidase [Carbohydrate transport and metabolism].
269893	GH27	1.05e-09	322	396	5	80	glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
407116	Glyco_hydro_36N	1.07e-08	76	183	68	181	Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCT61505.1|GH36	0.0	1	713	1	713
QGI88848.1|GH36	0.0	1	713	1	713
QGI57937.1|GH36	0.0	1	713	1	713
QGI75155.1|GH36	0.0	1	713	1	708
QKD48422.1|GH36	0.0	1	713	1	713

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2XN0_A	9.67e-24	92	542	131	557	Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN0_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN1_A Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_C Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_D Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM]
2XN2_A	1.28e-23	92	542	131	557	Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with galactose [Lactobacillus acidophilus NCFM]
6PHU_A	3.71e-22	94	587	143	599	SpAga wild type apo structure [Streptococcus pneumoniae TIGR4],6PHV_A Chain A, Alpha-galactosidase [Streptococcus pneumoniae TIGR4]
6PHW_A	1.50e-21	94	587	143	599	Chain A, Alpha-galactosidase [Streptococcus pneumoniae TIGR4],6PHX_A SpAga D472N structure in complex with raffinose [Streptococcus pneumoniae TIGR4],6PHY_A Chain A, Alpha-galactosidase [Streptococcus pneumoniae TIGR4],6PI0_A AgaD472N-Linear Blood group B type 2 trisaccharide complex structure [Streptococcus pneumoniae TIGR4]
3MI6_A	2.63e-21	92	587	128	587	Chain A, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_B Chain B, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_C Chain C, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_D Chain D, Alpha-galactosidase [Levilactobacillus brevis ATCC 367]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5ARP5|AGALG_EMENI	3.95e-24	207	542	211	552	Probable alpha-galactosidase G OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=aglG PE=2 SV=1
sp|G1UB44|MELA_LACAC	4.98e-23	92	542	131	557	Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
sp|P43467|AGAL1_PEDPE	1.08e-21	94	542	131	555	Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
sp|P27756|AGAL_STRMU	1.84e-21	68	566	101	560	Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3
sp|P43469|AGAL2_PEDPE	7.42e-21	269	587	279	580	Alpha-galactosidase 2 OS=Pediococcus pentosaceus OX=1255 GN=agaS PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000064	0.000000

TMHMM  Annotations     

There is no transmembrane helices in FFUJ_02016-t31_1-p1.