CAZyme Information: EXJ67513.1

Basic Information

• Species: Cladophialophora psammophila
• Lineage: Ascomycota; Eurotiomycetes; ; Herpotrichiellaceae; Cladophialophora; Cladophialophora psammophila
• CAZyme ID: EXJ67513.1
• CAZy Family: GH105
• CAZyme Description: beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
902	AMGX01000016|CGC3	101692.81	6.2945

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CpsammophilaCBS110553	13421	1182543	0	13421

• Gene Location: location=AMGX01000016:696848-699845(-)

Full Sequence      

MAVVTTELSSGWQCRQSDDGSVDAWLPVSKVPTTTHLVLQENAKIPDPFIDMNELAVRWV
AEKPWTYKTTFPSPTNRQPDARTDLVFEGLDTFATVTLNGHKILEADNMFLEYRVDVTNF
LLKPSSDSVLAGPNVLEIVFDSALLRGRKLVKRHSHEHNFIAHQTESSRIPVRKAQCHWG
WDWGPILITSGPWKPVLLQTYVVRIDDVWFQGEVSEDLKAVKGKLFSRVKCEKQRTQEGS
VVELSLSLDGKVLLELQAPIDNDGVATAEFRLERPALWYPRGYGKQCLYELEATLPTKGS
HPISKSRMIGFRRCELIQEPDEFGKSFYFRINNVDVFAGGSCWIPADSFIPRTGEDGYRK
WMELMIEGNQIMTRIWGGGIYEDDSFYNACDSLGILVWQDFCFACASYPTYPSFLSQVEQ
EARFNVRRLRSHPSLAIWAGNNEDYQIQEKHNLEYDYEGDKDPQSWLKTSFPARYIYEYL
LPKIVQEESPGAAYHPSSPWGDGKKTWDPTVGDIHQWNVWHGSMRRYQELSTIGGRFVSE
FGMEAYPHVSTIVSAIATDPKQQYPGSMPMDYHNRAVDHERRLLTYVAENFQIKYDLPSF
THLTQVMQSDAVGFAYRSWRREWGTPSKGQQKRKCGGVLVWQFNDTWPTMSWAVVDYYRV
KKPSFYAIKRSLKPLAVGVSRPFHEWTSGHVDPTGAIRENKFDLWVCSSRTGEDVKAGVV
VRAISIKTGRDVVDPIRKDGVTVKANGTTEILQGGEISLASASAARDPDPTTGMSFFDLR
AWDPYVIHASLLVDGRVVSSDTSWPHPIKYLDFSAPRDLKVQLSTAEGKDGGVTTATVTV
SATKPVHGFVFREGRDGATFSDNGFDVVPGEDVVVEVVSGGTSPEKPLRWTYIGAESASI
GL

Enzyme Prediction     

EC	3.2.1.25:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	686	2.2e-85	0.6196808510638298

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	1.16e-71	45	669	47	627	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407658	Mannosidase_ig	2.50e-12	700	810	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	5.21e-08	203	312	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
407625	Ig_mannosidase	0.002	849	880	27	59	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UQC76680.1|GH2	0.0	2	902	3	883
UKZ88363.1|GH2	0.0	1	902	1	887
QLI66193.1|GH2	0.0	1	900	1	874
UKZ51991.1|GH2	0.0	1	900	1	883
UKZ77816.1|GH2	0.0	1	900	1	883

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	2.55e-133	29	679	46	660	Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	3.05e-113	8	879	11	818	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	3.20e-113	8	879	13	820	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	3.27e-113	8	879	13	820	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	8.49e-113	8	879	11	818	Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5B7W2|MANBB_EMENI	1.15e-297	8	894	9	842	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
sp|A1D911|MANBB_NEOFI	1.05e-293	8	894	9	844	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
sp|Q0CCA0|MANBB_ASPTN	2.79e-293	8	894	9	842	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
sp|Q2TXB7|MANBB_ASPOR	4.09e-293	8	894	9	843	Beta-mannosidase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndB PE=3 SV=3
sp|B8NW36|MANBB_ASPFN	1.65e-292	8	894	9	843	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000031	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EXJ67513.1.