CAZyme Information: EQL33411.1

Basic Information

• Species: Blastomyces dermatitidis
• Lineage: Ascomycota; Eurotiomycetes; ; Ajellomycetaceae; Blastomyces; Blastomyces dermatitidis
• CAZyme ID: EQL33411.1
• CAZy Family: GH47
• CAZyme Description: alpha 1,3-glucosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
968		108938.15	5.8905

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_BdermatitidisATCC26199	9560	447095	380	9180

• Gene Location: location=GL538394:210322-213347(-)

Full Sequence      

MAAFRPSSTGWTLCLAILAILSCLVVPGEMVKHENFKTCSQSGFCSRNRAYADTVSSQGS
SWSAPYHLESNSIQFANGQLLGIIVKTIENNEKVNLPLTVSFLQSGVARVTVDEEKRIQG
DIQLRHDRKIRKERYNEAEKWVIVGGLELSSSAEIAKGTEAGITKVHYGPDNKFQAVIRH
SPFEVHFQRDGETHIQLNDKGLLNVEHWRPKVEKPTGDDSESEPQDAVDDSTWWEESFGG
NTDTKPRGPESVGLDITFPGYNHVFGIPEHADSLSLKETRGGDGHHTEPYRLYNSDVFEY
ELNSPMTLYGSIPFMQAHRKGSTVGVLWLNAAETWVDIVKTKASTNPLALGVVGKTSTQT
HWFSEAGQLDVFVFLGPTPQALSKSYGELTGFTQLPQHFAIGYHQCRWNYVTDEDVRDVD
RKFDLYQIPYDVIWLDIEYTDHKKYFTWDPLTFPDPDGMQKQLDDSGRKLVIIVDPHIKN
EANYPVVDELKSKGLGVKNKDGDIYDGWCWPGSSHWIDCFNPAAVSWWSTLFKYDKFKGT
HSNMFIWNDMNEPSVFNGPETTMPKDNIHHGGWEHRDLHNLNGMTFINATYHAMLERKKG
EVRRPFVLTRSFYAGTQRVGAMWTGDNQANWEHLAASLPMVLNNGIAGFPFAGADVGGFF
GNPSKELLTRWYQTGIFYPFFRAHAHIDTRRREPYLAGEPYMSIITQALQLRYQLLPAWY
TSFQQASIDGSPIVQPQYYVHPEDEAGFAIDDQLYLGSTGLLAKPIVAEGATSTDIYLAD
DEKYYDYFDYTVYQGAGKRHTVQAPLEKIPLLMQGGHIIPRKDRPRRSSELMKWDPYTLV
VTLDKNGQAEGTLYVDDGETFDYQNGAYIHRRFTFSESSLSSTDIGAKGTKTAQYLKTMA
GVRVEKIIIVDAPDAWESQSTVLVLEEGSQTGSQASLQWHAKQGGKAAYAVVKDPGVSIG
GLWKIDFS

Enzyme Prediction     

EC	3.2.1.20:27	3.2.1.84:26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	372	819	2e-149	0.9953161592505855

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395838	Glyco_hydro_31	0.0	372	819	1	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
269889	GH31_GANC_GANAB_alpha	0.0	391	860	1	467	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
224418	YicI	0.0	178	910	60	759	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
269890	GH31_glucosidase_II_MalA	1.94e-145	391	730	1	339	Alpha-glucosidase II-like. Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This subgroup also includes the MalA alpha-glucosidase from Sulfolobus solfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
215408	PLN02763	4.41e-119	377	897	164	669	hydrolase, hydrolyzing O-glycosyl compounds

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSS76196.1|GH31	0.0	1	968	1	968
QSS58256.1|GH31	0.0	1	968	1	968
QSS53224.1|GH31	0.0	1	968	1	968
BCR98911.1|GH31	0.0	1	967	1	963
BCS11223.1|GH31	0.0	1	967	1	963

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DKZ_A	0.0	29	967	3	950	Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5DL0_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495]
5DKX_A	0.0	29	967	3	950	Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5DKY_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495],5JQP_A Chain A, Alpha glucosidase-like protein [Thermochaetoides thermophila DSM 1495]
7KRY_A	1.26e-249	8	965	6	940	Chain A, Neutral alpha-glucosidase AB [Mus musculus],7KRY_C Chain C, Neutral alpha-glucosidase AB [Mus musculus]
5IEF_A	5.19e-249	31	965	1	909	Murine endoplasmic reticulum alpha-glucosidase II with N-butyl-1-deoxynojirimycin [Mus musculus]
5IEG_A	5.36e-249	31	965	1	909	Murine endoplasmic reticulum alpha-glucosidase II with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9US55|GLU2A_SCHPO	6.56e-301	32	909	28	883	Glucosidase 2 subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gls2 PE=1 SV=1
sp|Q94502|GANAB_DICDI	1.38e-250	15	910	7	896	Neutral alpha-glucosidase AB OS=Dictyostelium discoideum OX=44689 GN=modA PE=3 SV=1
sp|Q9FN05|PSL5_ARATH	5.43e-250	32	885	22	845	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
sp|Q8BHN3|GANAB_MOUSE	3.63e-248	17	965	19	941	Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1
sp|P79403|GANAB_PIG	3.26e-246	1	965	7	941	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000277	0.999704	CS pos: 30-31. Pr: 0.8877

TMHMM  Annotations     

Start	End
7	29