CAZyme Information: EPrPVT00000022082-p1

Basic Information

• Species: Phytopythium vexans
• Lineage: Oomycota; NA; ; Pythiaceae; Phytopythium; Phytopythium vexans
• CAZyme ID: EPrPVT00000022082-p1
• CAZy Family: GH85
• CAZyme Description: Endo-1,3(4)-beta-glucanase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1121		121913.07	4.4166

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PvexansDAOMBR484	11991	1223560	34	11957

• Gene Location: location=PvexDAOMBR484_SC0786:9316-12752(+)

Full Sequence      

MRCRSQLPLLLAALALLCGAVHGALNLTLSRAQPLPXXXXQLSTALFASSEEADGSQRAL
ADXXXXPFPTGAWWTNLVLARGESAVVAMPYVFRVLQHRLHVCFPFRVVTPHVIQNGFIS
QLVVSSAAETGDVAPPNAHQVVGFDTFGATVRFSRGEADEFNVFLVRGSPYISMEFSRSR
PVIESTDGLSITRIKKNDGLVFMDGSDVDFAVFSVMVSNGQMWYIFASDLELDLQMDGAG
RVVAEKPFTGALRIALALEATTMPYLLESASVYPVGGDVSFTVDNVDDSQIADMEFRWKT
KSFASYGTTAESSARRSAELLMLALPHHMDTLQIQEDKQAEHANSVVDTLRYTSIRGLMK
GVLGNVWYMRETLPAVQWNFADDGLFSDEYDPKEIDKSSVDDEKEKREAMRADVKSKIIS
SLPDDIEKYPVLTDDSYNFGKQIGREARLLLIAHKFEQDHIVSQVLEKMEKELEQRLKGM
NGDKFVYDETYGGVVTTDGIRNQDADFGNGYYNDHHFHYGYFIYALAVVRKFDPEFIQAN
ALACAMLLADIGTPLDAQMAFFDGLPAKQFFPPARHKDWFMGHSYASGLFPMEVGKSQES
SSECVNAYYAMMLFSSLDADADDASSYHQYTRLMLAMELRSTKMYWHMQENSQIYEPIFA
KNAMVGVVGEMSVVYSTWFGDHAVYVHGINLLPFTPITTELLDEPYVVHEYPLLDRELDG
LDSNNIWRSIVVLDHAILDGAKAWDELVETVTAYDTWNSPSNSMYWIATRPSWYSQKNRD
VLAHPVTDQDDMCFGYPECSTAGDNGTALLCCSTLPGCCPSALGCCPKEDPVDIPANACF
GELQCGLLGLGCCNTIDGCCEPDPVSGTVLGCCKDQHPVFLPSAKPAASANDTQSVCYGE
PQCAAAKLDCCSVPGGCCAGSGPKLDCCSTEPIAAAPTGTDSPTEDEAATCHSQPACAAA
GLDCCATDAGCCEPDPKTGAVLDCCVAVTSSPARAGSAEVDTCHDEPVCAAAKLLCCDTD
AGCCAPDPVSGAVLDCCKSASASTRDGDASTAGETDINGKTLTRLFIGVAALGVVGLAVY
AAITWYRRRGYNTLDRDMRALYCAGMLIAVIAFFVFLIIMG

Enzyme Prediction     

No EC number prediction in EPrPVT00000022082-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	54	766	7.2e-152	0.9903536977491961

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
407548	Glyco_hydro81C	2.62e-80	412	774	4	348	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
227785	Acf2	2.17e-77	60	767	82	748	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
397619	Glyco_hydro_81	8.61e-40	67	381	14	317	Glycosyl hydrolase family 81. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein ENGL1, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ29064.1|GH81	0.0	34	1117	33	1076
CCA18874.1|GH81	9.40e-229	67	883	29	853
AGP40408.1|GH81	2.19e-103	67	771	79	702
AUX36386.1|GH81	5.59e-103	67	771	90	713
CAN97272.1|GH81	3.04e-98	29	771	63	718

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4K3A_A	3.57e-50	69	783	42	731	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K3A_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5XBZ_A	4.58e-50	69	783	67	756	Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XBZ_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose [Rhizomucor miehei],5XC2_A Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_B Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_C Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei],5XC2_D Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminarihexaose [Rhizomucor miehei]
4K35_A	1.55e-48	69	783	42	731	The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei],4K35_B The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase [Rhizomucor miehei]
5V1W_A	2.78e-24	61	754	42	665	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5T4A_A	2.97e-24	61	754	62	685	Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125],5T4G_A Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AIR7|ENG1_CANAL	2.59e-39	62	766	455	1133	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1
sp|Q09850|ENG2_SCHPO	4.49e-38	165	768	145	697	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
sp|Q12168|ENG2_YEAST	2.21e-36	213	745	258	750	Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1
sp|D4AZ24|ENG1_ARTBC	4.91e-36	119	775	298	904	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
sp|Q9UT45|ENG1_SCHPO	5.71e-35	129	787	131	748	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000258	0.999705	CS pos: 23-24. Pr: 0.9682

TMHMM  Annotations     

Start	End
7	29
1065	1087
1100	1119