CAZyme Information: EPrPVT00000018904-p1

Basic Information

• Species: Phytopythium vexans
• Lineage: Oomycota; NA; ; Pythiaceae; Phytopythium; Phytopythium vexans
• CAZyme ID: EPrPVT00000018904-p1
• CAZy Family: GH30
• CAZyme Description: Beta-glucan synthesis-associated protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
716		77572.35	4.5497

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PvexansDAOMBR484	11991	1223560	34	11957

• Gene Location: location=PvexDAOMBR484_SC0348:25319-27469(+)

Full Sequence      

MRVWTAALAVVGLAAPVDATKLIETRSGLRPWVDADTPARAYTVTSSRGATWDLVMSDEF
NDEGRDFAAGKDHLWTALDMPDGVNAALEYYSPNMSTTVTESDGRGVFQIEVQEVQYGIN
YSAYNIWAHPPKYENHTMYYRAAMVQSWNKFCFQGGRMEVSAQLPGAVTSASGNPDLGNN
NVRVETTKFYPTWPGIWLLGNLGRALFTGTTSRMWPWSYNECNTNYSSNQKISACNSSPG
HGLNPNQGRGAPEIDLLEGGGTAISTSIQLAPGMPDDFRMFSATNDTNTACFYSAACTTS
GANFPGIPNATYAKRGHNSWYQGLRYAPNTLCSSEGDQIQDATTVKKNVAAGIKSNNCNG
VNTCPASKDGYSDLDLIDGEGSKYWGINDVGGCMPVINGYMGAYLCDADNTDEKCSSPLS
SGVGKSNVMESFHYQMDAISANWGIQLGAYTSYVTYQLEWVMGDSGYIRWMVEDVVVFEI
PAESIVNPPQDDAKSNPVKIMIEEPMYIIFNVALSTSWGTSPPNAGKTCRGDGTDATTNK
ICDSFPMYMKIDYIRIYQDLSENSTMAVGCDPSTHPTAQYITDNIDDYTNTNNKWIEVTG
GANCHSTDDCTVGTSTVLTGMCSSGKCRCLSSGAWGGPRCTMAFSDTSAANGYGPSIAVS
WLVAIAGIAALVFSIYRIAKARATQRERQTEAENESKVTTSMRSGIEVESTQVKAL

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	58	281	2.1e-65	0.6073619631901841

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	3.13e-69	26	592	94	480	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.73e-55	54	558	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	1.02e-10	428	557	118	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	7.47e-10	33	344	30	283	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	2.92e-06	57	218	2	113	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA25842.1|GH16_2	2.45e-268	21	678	18	675
UIZ27996.1|GH16	3.69e-258	25	680	32	686
UIZ28151.1|GH16	8.41e-257	25	698	32	723
UIZ27810.1|GH16	6.96e-255	25	689	67	743
UIZ28149.1|GH16	1.43e-247	25	675	31	698

PDB Hits     

EPrPVT00000018904-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P32486|KRE6_YEAST	2.39e-40	33	626	307	715	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P33336|SKN1_YEAST	1.14e-37	33	626	359	767	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	4.36e-34	33	592	332	721	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	1.80e-32	33	592	336	718	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|O13941|YEPB_SCHPO	1.82e-32	33	600	219	615	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000284	0.999674	CS pos: 19-20. Pr: 0.9725

TMHMM  Annotations     

Start	End
657	679