CAZyme Information: EPrPRT00000022527-p1

Basic Information

• Species: Pythium arrhenomanes
• Lineage: Oomycota; NA; ; Pythiaceae; Pythium; Pythium arrhenomanes
• CAZyme ID: EPrPRT00000022527-p1
• CAZy Family: GT19
• CAZyme Description: Alpha

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1021		114166.01	6.5651

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_ParrhenomanesATCC12531	13857	1223556	52	13805

• Gene Location: location=ParrATCC12531_SC02236:298-3882(+)

Full Sequence      

MKRSSHSRQRSASLEEEIFASTSGSGGFVTSPTRRQETTERSPAPLASFLEQQQQRQRYG
DPVPPGDEPLDESDSDESSSDDEDGESSSEESSSCESDGAALRGDSELHRRTAPPATASP
ARPRSGSLSALPELLVNIQELQRQLSMVQIQTEKPQEEILPPMGDSRHRLIMVSNRLPIS
FQRNETTGEWSFQMSSGGLVTALNGIRDEVPFIWIGWLGEEIAHEDQPKVREKLAQEFNC
VPVFLSKEVASRYYNDFSNDILWPIFHYVPLPLFRAGSEKKFDFRLWDAYKLANKRFAQA
VNQVYREGDYVWIHDYHLMNLPSLLRKRHPKCKIGWFLHTPFPSAEMYRALPVGKQILEG
LLGADLLGFHTYDYARHFLSACGRISGATSSPKGIEWDDHFSAVGVFPIGIDPEHFEAIL
AADETHRRIEELTQKFKGCKXXXXTRPTKFKGCKIIIGVDRMDYIKGIPHKLLAMERFLS
MYPERRESVVLLQIAVPSRTGVIEYQNLAASVNEMVGRINGRHEYVMCQSQPCEPHREGP
GVLILSEFAGSAQSLSGAIRVNPWNTSDXXXXRVNPWNTSDMASALEYALTLPLMEREYR
QTNLHRYVKTHTASFWGKSFLADLEYTVSRPSSSRKLVKLPTMDVINAYARSKRRMIVLD
YEGTLLEEAHQRATIAPPKPFIKRMMESLASDPKNTVLLVSGHERGVIGNEWEMMTEDHD
PSWKKVVRPIMQYFTERTPGSFIETKESSLTWHYGETDPIFGPMQARDMQVNLEDVLCSL
PLEVLQGTNNVEVRLQGITKTVIIDEILKLYEQLQHQQPVSPATAGGSTRSTNTKTKTKQ
PPIDFVFCAGNGLEDDDLFTFFQEHHDHAKLQATKASTTTTTMNTTTKTSLLTATSLPPG
VARPSTQPLAASTPTPVPTPTPTPTVEMRSLLKGSTALAKNANVYTCHIGANKTEAMYYL
ERPSDLVRVLRSLSAQSREGYGAPESADAMGTHRLDGDLPGSSPTVAYSSAAAPTHTPSP
P

Enzyme Prediction     

EC	2.4.1.15:146

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	167	523	8.3e-130	0.7073684210526315

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340820	GT20_TPS	0.0	169	625	1	463	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
395781	Glyco_transf_20	0.0	169	626	2	470	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.
215556	PLN03064	0.0	156	977	85	930	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional
184712	PRK14501	0.0	169	859	2	687	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
215555	PLN03063	0.0	167	863	10	721	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCA16456.1|GT20	0.0	133	975	133	968
CCA20339.1|GT20	0.0	133	979	46	933
UIZ21560.1|GT20	6.02e-279	133	625	144	653
QOX06485.1|GT20	2.10e-201	161	865	68	780
AJA04606.1|GT20	7.97e-193	133	865	67	837

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HVM_A	3.35e-134	169	624	13	474	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
6JBI_A	1.25e-130	169	624	1	462	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5HUV_A	1.67e-127	169	624	5	467	Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]
5HVO_A	6.67e-127	164	624	7	474	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HUT_A	9.06e-127	169	624	5	467	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q54K57|TPSA_DICDI	3.87e-176	169	809	17	680	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1
sp|Q9SYM4|TPS1_ARATH	3.22e-157	166	863	90	809	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 OS=Arabidopsis thaliana OX=3702 GN=TPS1 PE=1 SV=1
sp|Q9FZ57|TPS2_ARATH	6.62e-148	167	863	11	723	Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2 OS=Arabidopsis thaliana OX=3702 GN=TPS2 PE=3 SV=1
sp|O23617|TPS5_ARATH	2.41e-138	162	867	54	800	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 5 OS=Arabidopsis thaliana OX=3702 GN=TPS5 PE=1 SV=2
sp|Q00764|TPS1_YEAST	1.01e-136	164	634	12	490	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000060	0.000000

TMHMM  Annotations     

There is no transmembrane helices in EPrPRT00000022527-p1.