CAZyme Information: EPrPRT00000013575-p1

Basic Information

• Species: Pythium arrhenomanes
• Lineage: Oomycota; NA; ; Pythiaceae; Pythium; Pythium arrhenomanes
• CAZyme ID: EPrPRT00000013575-p1
• CAZy Family: AA17
• CAZyme Description: Beta-glucan synthesis-associated protein.

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
800		87832.63	6.8785

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_ParrhenomanesATCC12531	13857	1223556	52	13805

• Gene Location: location=ParrATCC12531_SC00029:39961-42536(-)

Full Sequence      

MVARVCPTLVLAAACYASLAAASTGQGSPQIATDTTATGIDSGSGSSGVDGMATVSQPRR
PIDELSAISGIPLWVDPTTPDDKQTYVSSRGETWELVMSDEFGDPARNFTPGRDHLWTSL
EKPDGVNGALEFYAHNMSYVTCEPSKSDTDTTSTNTTAVCYLTMEVQDVVSRIEVYNMYS
RPPSMQIAKFYYRAAMMQTWNKFCFQGGMVEVRAQLPGAVSKKSKNPDVAGGPNARTQTG
AFYPTWPGIWMMGNLGRAIFSGSTSRMWPFSYNECNPDAFDPKNQRISACESNPGHGLNP
NQGRGAPEIDLLEGGGVAISSSIQIGPGLKVKYRRIPVNLTIPNEYKDCVYNGACQTPGA
NLPNVPTALYKPRKYQTWYKGLRYSSNSFCAMDPKEKQTYAQISAAIVNITEATCSVQTC
PAXXXXAAIVNITKDTCSVETCPASRDVQADLSFIPNDKTKARWGINSKGTCAAIMNNYM
GSFLCDPDSTNPMCETPRNATTPKTNVEWVMGDTGYLRWMLYGNPIFEIPAESVVKGPQA
KTDPNPQLTFPEEPMYLIFNVALSNQWGAMPPNAGKGPCGMMVGKPEIDNQTKLVCDSFP
MYMKIDYIRVYQDKSPNSTMAVGCDPKTHPTRQWIFDHIKEYEDDQNPYVEVDGGAYCRN
DLDCMIPFNTTVNFATGNCVEWRCKCNTKYWGGPRCTTALKITDLSTAAKQAPSYGPPLY
LSITIGSISIVMTCAIIAFCTRRRRRKDVELQQKLAKMKTELTIEKASLLPSSDFDEIEL
ARHSNATPQSRYQPLSAKGA

Enzyme Prediction     

EC	-	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	100	371	1.5e-56	0.7239263803680982

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	3.11e-55	75	648	98	479	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.43e-42	96	612	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185693	GH16_laminarinase_like	2.34e-09	507	611	165	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	7.69e-08	191	275	102	156	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.48e-05	192	254	64	103	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UIZ28149.1|GH16	3.73e-249	69	751	33	712
UIZ28152.1|GH16	5.93e-239	69	733	22	681
UIZ28151.1|GH16	9.05e-237	63	733	28	696
UIZ24235.1|GH16_2	2.95e-230	63	744	27	696
UIZ27810.1|GH16	2.11e-222	64	728	64	722

PDB Hits     

EPrPRT00000013575-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P33336|SKN1_YEAST	1.67e-31	75	642	359	739	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P32486|KRE6_YEAST	7.25e-30	75	662	307	707	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87023|KRE6_CANAX	7.43e-29	75	662	332	737	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P87024|SKN1_CANAX	9.75e-29	75	643	336	715	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|O13789|YEOB_SCHPO	5.27e-24	75	642	224	612	Uncharacterized beta-glucan synthesis-associated protein C17G6.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17G6.11c PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000824	0.999145	CS pos: 22-23. Pr: 0.9503

TMHMM  Annotations     

Start	End
718	740